Electric impedance sensing in cell-substrates for rapid and selective multipotential differentiation capacity monitoring of human mesenchymal stem cells

2012 ◽  
Vol 34 (1) ◽  
pp. 63-69 ◽  
Author(s):  
Stephan Reitinger ◽  
Jürgen Wissenwasser ◽  
Werner Kapferer ◽  
Rudolf Heer ◽  
Günter Lepperdinger
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Electric Impedance ◽  
Impedance Sensing ◽  
Differentiation Capacity ◽  
Multipotential Differentiation

scholarly journals Differentiation Capacity of Human Mesenchymal Stem Cells into Keratocyte Lineage

2019 ◽  
Vol 60 (8) ◽  
pp. 3013 ◽  
Author(s):  
Aurelie Dos Santos ◽  
Alis Balayan ◽  
Martha L. Funderburgh ◽  
John Ngo ◽  
James L. Funderburgh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Mesenchymal Stem Cells ◽  
Differentiation Capacity

scholarly journals Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan

Aging Cell ◽  
2007 ◽  
Vol 6 (6) ◽  
pp. 745-757 ◽  
Author(s):  
Christine Fehrer ◽  
Regina Brunauer ◽  
Gerhard Laschober ◽  
Hermann Unterluggauer ◽  
Stephan Reitinger ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Oxygen Tension ◽  
Human Mesenchymal Stem Cells ◽  
Differentiation Capacity

scholarly journals Interactions of Human Endothelial and Multipotent Mesenchymal Stem Cells in Cocultures

2010 ◽  
Vol 4 (1) ◽  
pp. 190-198 ◽  
Author(s):  
Christina Ern ◽  
Vera Krump-Konvalinkova ◽  
Denitsa Docheva ◽  
Stefanie Schindler ◽  
Oliver Rossmann ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Culture Media ◽  
Human Mesenchymal Stem Cells ◽  
Time Lapse ◽  
Cell Interactions ◽  
Differentiation Capacity ◽  
Multipotent Mesenchymal Stem Cells ◽  
Time Lapse Imaging

Current strategies for tissue engineering of bone rely on the implantation of scaffolds, colonized with human mesenchymal stem cells (hMSC), into a recipient. A major limitation is the lack of blood vessels. One approach to enhance the scaffold vascularisation is to supply the scaffolds with endothelial cells (EC). The main goal of this study was to establish a coculture system of hMSC and EC for the purposes of bone tissue engineering. Therefore, the cell behaviour, proliferation and differentiation capacity in various cell culture media as well as cell interactions in the cocultures were evaluated. The differentiation capacity of hMSC along osteogenic, chondrogenic, and adipogenic lineage was impaired in EC medium while in a mixed EC and hMSC media, hMSC maintained osteogenic differentiation. In order to identify and trace EC in the cocultures, EC were transduced with eGFP. Using time-lapse imaging, we observed that hMSC and EC actively migrated towards cells of their own type and formed separate clusters in long term cocultures. The scarcity of hMSC and EC contacts in the cocultures suggest the influence of growth factor-mediated cell interactions and points to the necessity of further optimization of the coculture conditions.

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