A label-free electrochemical immunoassay for carcinoembryonic antigen (CEA) based on gold nanoparticles (AuNPs) and nonconductive polymer film

2007 ◽  
Vol 22 (6) ◽  
pp. 1061-1067 ◽  
Author(s):  
Hao Tang ◽  
Jinhua Chen ◽  
Lihua Nie ◽  
Yafei Kuang ◽  
Shouzhuo Yao
Keyword(s):  
Gold Nanoparticles ◽  
Carcinoembryonic Antigen ◽  
Polymer Film ◽  
Electrochemical Immunoassay ◽  
Label Free

Electrochemical immunoassay of carcinoembryonic antigen based on TiO2–graphene / thionine / gold nanoparticles composite

2012 ◽  
Vol 90 (7) ◽  
pp. 608-615 ◽  
Author(s):  
Ke-Jing Huang ◽  
Zhi-Wei Wu ◽  
Ying-Ying Wu ◽  
Yan-Ming Liu
Keyword(s):  
Gold Nanoparticles ◽  
Carcinoembryonic Antigen ◽  
Linear Response ◽  
Multilayer Film ◽  
Electrostatic Force ◽  
Carbon Electrode ◽  
Electrochemical Immunoassay ◽  
Amino Groups ◽  
Amperometric Immunosensor ◽  
Highly Sensitive

A novel multilayer film based on gold nanoparticles (AuNPs), thionine (Thi), and TiO2–graphene (TiO2–Gr) was exploited to develop a highly sensitive amperometric immunosensor for detecting carcinoembryonic antigen (CEA). Firstly, Nafion–TiO2–Gr homogeneous composite was dropped on the surface of a glassy carbon electrode (GCE). Then Thi was chemisorbed by the TiO2–Gr–Nafion composite. Furthermore, the negative ly charged AuNPs were chemisorbed onto Thi film through the electrostatic force with the amino groups of Thi. Cyclic voltammetry (CV) was employed to characterize the assembly process and the performance of the immunosensor. Because of the synergistic effect of the AuNPs, Thi, and the unique properties of TiO2–Gr, the obtained immunosensor exhibited a wide linear response to CEA in two ranges from 0.1 to 10.0 ng mL−1 and from 10.0 to 120.0 ng mL−1 with a relatively low detection limit of 0.01 ng mL−1 (S/N = 3), as well as good stability and repeatability.

scholarly journals Ultrasensitive and label free electrochemical immunosensor for detection of ROR1 as an oncofetal biomarker using gold nanoparticles assisted LDH/rGO nanocomposite

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rozita Abolhasan ◽  
Balal Khalilzadeh ◽  
Hadi Yousefi ◽  
Sahar Samemaleki ◽  
Forough Chakari-Khiavi ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Flexible Structures ◽  
Modified Electrodes ◽  
Electrochemical Immunosensor ◽  
Lymphocytic Leukemia ◽  
Orphan Receptor ◽  
Detection Sensitivity ◽  
Label Free ◽  
Serum Samples ◽  
Limit Of Quantification

AbstractIn the present article, we developed a highly sensitive label-free electrochemical immunosensor based on NiFe-layered double hydroxides (LDH)/reduced graphene oxide (rGO)/gold nanoparticles modified glassy carbon electrode for the determination of receptor tyrosine kinase-like orphan receptor (ROR)-1. In this electrochemical immunoassay platform, NiFe-LDH/rGO was used due to great electron mobility, high specific surface area and flexible structures, while Au nanoparticles were prepared and coated on the modified electrodes to improve the detection sensitivity and ROR1 antibody immobilizing (ROR1Ab). The modification procedure was approved by using cyclic voltammetry and differential pulse voltammetry based on the response of peak current to the step by step modifications. Under optimum conditions, the experimental results showed that the immunosensor revealed a sensitive response to ROR1 in the range of 0.01–1 pg mL−1, and with a lower limit of quantification of 10 attogram/mL (10 ag mL−1). Furthermore, the designed immunosensor was applied for the analysis of ROR1 in several serum samples of chronic lymphocytic leukemia suffering patients with acceptable results, and it also exhibited good selectivity, reproducibility and stability.

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