Development of a high analytical performance-tyrosinase biosensor based on a composite graphite–Teflon electrode modified with gold nanoparticles

2006 ◽  
Vol 22 (5) ◽  
pp. 730-736 ◽  
Author(s):  
V. Carralero ◽  
M.L. Mena ◽  
A. Gonzalez-Cortés ◽  
P. Yáñez-Sedeño ◽  
J.M. Pingarrón
Keyword(s):  
Gold Nanoparticles ◽  
Analytical Performance ◽  
Tyrosinase Biosensor ◽  
High Analytical Performance

Development of a tyrosinase biosensor based on gold nanoparticles-modified glassy carbon electrodes

2005 ◽  
Vol 528 (1) ◽  
pp. 1-8 ◽  
Author(s):  
V. Carralero Sanz ◽  
Ma Luz Mena ◽  
A. González-Cortés ◽  
P. Yáñez-Sedeño ◽  
J.M. Pingarrón
Keyword(s):  
Gold Nanoparticles ◽  
Glassy Carbon ◽  
Carbon Electrodes ◽  
Glassy Carbon Electrodes ◽  
Tyrosinase Biosensor

scholarly journals Evaluation of GENECUBE® HQ SARS-CoV-2 for anterior nasal samples and saliva samples with a new rapid examination protocol

2021 ◽  
Author(s):  
Asami Naito ◽  
Yoshihiko Kiyasu ◽  
Yusaku Akashi ◽  
Akio Sugiyama ◽  
Masashi Michibuchi ◽  
...  
Keyword(s):  
Analytical Performance ◽  
Process Time ◽  
Identification System ◽  
Rt Pcr ◽  
Examination Protocol ◽  
Prospective Comparison ◽  
Total Process ◽  
Extraction Processes ◽  
Polymerase Chain ◽  
High Analytical Performance

AbstractIntroductionGENECUBE® is a rapid molecular identification system, and previous studies demonstrated that GENECUBE® HQ SARS-CoV-2 showed excellent analytical performance for the detection of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) with nasopharyngeal samples. However, other respiratory samples have not been evaluated.MethodsThis prospective comparison between GENECUBE® HQ SARS-CoV-2 and reference real-time reverse transcriptase polymerase chain reaction (RT-PCR) was performed for the detection of SARS-CoV-2 using anterior nasal samples and saliva samples. Additionally, we evaluated a new rapid examination protocol using GENECUBE® HQ SARS-CoV-2 for the detection of SARS-CoV-2 with saliva samples. For the rapid protocol, in the preparation of saliva samples, purification and extraction processes were adjusted, and the total process time was shortened to approximately 35 minutes.ResultsFor 359 anterior nasal samples, the total-, positive-, and negative concordance of the two assays was 99.7% (358/359), 98.1% (51/52), and 100% (307/307), respectively. For saliva samples, the total-, positive-, and negative concordance of the two assays was 99.6% (239/240), 100% (56/56), and 99.5% (183/184), respectively. With the new protocol, total-, positive-, and negative concordance of the two assays was 98.8% (237/240), 100% (56/56), and 98.4% (181/184), respectively. In all discordance cases, SARS-CoV-2 was detected by additional molecular examinations.ConclusionGENECUBE® HQ SARS-CoV-2 provided high analytical performance for the detection of SARS-CoV-2 in anterior nasal samples and saliva samples.

scholarly journals Comparison of Single- and Mixed-Sized Gold Nanoparticles on Lateral Flow Assay for Albumin Detection

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 209
Author(s):  
Sasima Chotithammakul ◽  
Michael B. Cortie ◽  
Dakrong Pissuwan
Keyword(s):  
Chronic Kidney Disease ◽  
Gold Nanoparticles ◽  
Test Line ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Analytical Performance ◽  
Detection Mechanism ◽  
Multiple Factors ◽  
20 Nm ◽  
Target Analyte

The sensitivity and reproducibility of the lateral flow assay can be influenced by multiple factors, such as the size of gold nanoparticles (GNPs) employed. Here, we evaluated the analytical performance of single-sized and mixed-sized GNPs using a simple lateral flow assay (LFA) platform. This platform was used as a model assay to diagnose albumin levels and demonstrate the analytical performance of single-sized and mixed-sized GNPs in LFA tests. Two sizes of GNPs@anti-bovine serum albumin (BSA) conjugate proteins were mixed at different ratios. The unique optical properties of the GNPs induced a distinguishing color-shedding effect on the single- and mixed-sized GNPs@anti-BSA conjugates interacting with the target analyte BSA spotted on the test line. The use of mixed-sized GNPs@anti-BSA conjugates enhanced signal relative to the 20 nm GNPs, and provided superior stability compared with solely employing the large GNPs (50 nm). The proposed platform in this study could provide an efficient BSA detection mechanism that can be utilized as a model biomarker for confronting chronic kidney disease.

Electrochemical sensors of octylphenol based on molecularly imprinted poly(3,4-ethylenedioxythiophene) and poly(3,4-ethylenedioxythiophene–gold nanoparticles)

RSC Advances ◽  
2015 ◽  
Vol 5 (71) ◽  
pp. 57671-57677 ◽  
Author(s):  
Yanhui Pan ◽  
Faqiong Zhao ◽  
Baizhao Zeng
Keyword(s):  
Gold Nanoparticles ◽  
Electrochemical Sensors ◽  
Graphene Nanoribbons ◽  
Analytical Performance ◽  
Molecularly Imprinted ◽  
Octyl Phenol

Two molecularly imprinted electrochemical sensors are fabricated by using EDOT and EDOT–AuNPs as monomers, respectively. The sensors show good analytical performance for OP sensing. Note: graphene nanoribbons (GNRs), 3,4-ethylenedioxythiophene (EDOT), 4-tert-octyl-phenol (OP).

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