Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India

2007 ◽  
Vol 98 (7) ◽  
pp. 1339-1345 ◽  
Author(s):  
Kishore Das ◽  
Ashis K. Mukherjee
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Contaminated Soil ◽  
Crude Petroleum ◽  
North East India ◽  
North East ◽  
Oil Biodegradation ◽  
Petroleum Oil ◽  
Biodegradation Efficiency

scholarly journals A Bioremediation Study of Raw and Treated Crude Petroleum Oil Polluted Soil with Aspergillus niger and Pseudomonas aeruginosa

2018 ◽  
Vol 19 (2) ◽  
pp. 226-235 ◽  
Author(s):  
Modupe Ojewumi ◽  
Ejemen Anenih ◽  
Olugbenga Taiwo ◽  
Bosede Adekeye ◽  
Olugbenga Awolu ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Aspergillus Niger ◽  
Polluted Soil ◽  
Crude Petroleum ◽  
Petroleum Oil

Structural and physico-chemical characterization of a dirhamnolipid biosurfactant purified from Pseudomonas aeruginosa: application of crude biosurfactant in enhanced oil recovery

RSC Advances ◽  
2016 ◽  
Vol 6 (74) ◽  
pp. 70669-70681 ◽  
Author(s):  
Debananda Gogoi ◽  
Pabitra Bhagowati ◽  
Pronob Gogoi ◽  
Naba K. Bordoloi ◽  
Abu Rafay ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Enhanced Oil Recovery ◽  
Oil Recovery ◽  
Structural Characterization ◽  
Contaminated Soil ◽  
Chemical Characterization ◽  
Biotechnological Application ◽  
Physico Chemical ◽  
Petroleum Oil

The present study describes the structural characterization and biotechnological application of a dirhamnolipid biosurfactant produced byPseudomonas aeruginosastrain NBTU-01 isolated from a petroleum oil-contaminated soil sample.

scholarly journals TREATMENT PETROLEUM OIL CONTAMINATED SOIL BY INOCULATION OF DIFFERENT BACTERIAL STRAINS

2019 ◽  
Vol 2 (1) ◽  
pp. 1
Author(s):  
Pankaj Kumar Jain
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Contaminated Soil ◽  
Bacterial Count ◽  
Soil Samples ◽  
Total Bacterial Count ◽  
Soil Reaction ◽  
Available Phosphorus ◽  
Bacterial Strains ◽  
Degrading Bacteria ◽  
Petroleum Oil

Petroleum oil contains a large number of poly cyclic hydrocarbons (PAH's) that are toxic to living beings. The complete degradation of petroleum oil required a population of microorganisms in the soil. In the present investigation petroleum oil contaminated soil samples were incubated with four bacterial strains (Mycobacterium sp., Pseudomonas aeruginosa, Alcaligenes faecalis and Enterobacter cloacae) to study the bioremediation efficacy. The soil samples were analyzed for soil reaction (pH), soil moisture content, soil organic carbon (SOC), available phosphorus (P), total petroleum hydrocarbon content (TPH), total bacterial count (TBC) and total petroleum degrading bacteria at the interval of 0 days (initial), 2 weeks, 4 weeks, 6 weeks and 8 weeks prior and after treatment by bacteria. Values obtained reveals that there was a clear modulating effect of bacteria on above determinations. Maximum decrements in TPH (86%), soil pH (18.2%) and SOC (40%) were recorded in Pseudomonas aeruginosa inoculated samples.

scholarly journals Microbial studies of biosurfactant producing bacteria from crude oil contaminated soil

2021 ◽  
Vol 25 (9) ◽  
pp. 1729-1735
Author(s):  
K.I. Ogru ◽  
P.G. Olannye
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Crude Oil ◽  
Contaminated Soil ◽  
Heterotrophic Bacteria ◽  
Metal Removal ◽  
Soil Samples ◽  
Optimum Growth ◽  
The Mean ◽  
Hydrocarbon Utilizing Bacteria

Soils contaminated with crude oil collected from three sites in Delta State were analyzed using serial dilution and pour plate methods. The physicochemical characteristics of the soil were determined using standard methods. The mean heterotrophic bacteria count and the mean hydrocarbon utilizing bacteria count were also determined. Blood haemolysis, foaming activity, oil spreading techniques and emulsification assay was used to screen the microorganisms for biosurfactant production. The biosurfactant producing bacteria were selected and their effect on metal removal and growth kinetics was also determined. The pH of the contaminated soil samples ranged 4.82 to 5.62. The mineral elements such as potassium (k+) and sodium (Na+) ranged from 0.20 to 0.80 Meq/100g and 0.07 to 0.81 Meq/100g respectively. The heavy metal content such as zinc (Zn2+) and lead (Pb2+) ranged from 10.13 to 19.24 mg/kg and 19.24 to 49.63 mg/kg respectively. Organic carbon and THC ranged from 5.44 to 6.87 % and 2720.00 to 3110.00 mg/kg respectively. The mean heterotrophic bacteria and hydrocarbon utilizing bacteria ranges from 1.4 ± 0.4 x 106 to 1.6 ± 0.7 x 106 and 1.0 ± 0.2 x 106 to 1.2 ± 0.4 × 106 respectively in the soil samples. Bacteria isolated and identified from the three sites include Corynebacterium spp., Bacillus subtilis., Pseudomonas aeruginosa, Micrococcus varians, Streptococcus spp., Klebsiella spp. and Enterobacter spp. with Bacillus subtilis and Pseudomonas aeruginosa having the highest prevalence and among these isolates only Corynebacterium spp., Bacillus subtilis., Pseudomonas aeruginosa and Micrococcus varians showed positive result for the production of biosurfactant. Bacillus subtilis had the highest metal removal capacity of 29.44% of 100 ppm of lead. Corynebacterium spp. and Bacillus subtilis had an optimum growth at pH 8 and 7 respectively while Pseudomonas aeruginosa and Micrococcus varians has an optimum growth at pH.

Mikrobizide und viruzide Aufbereitung von Dialysegeräten

2018 ◽  
Vol 22 (02) ◽  
pp. 82-89
Author(s):  
Friedrich von Rheinbaben ◽  
Oliver Riebe ◽  
Johanna Köhnlein ◽  
Sebastian Werner
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Phase 3 ◽  
Aspergillus Brasiliensis

ZusammenfassungZentrales Bauteil des Genius® 90 Therapie Systems ist der sogenannte Genius-Tank, dem die frische Dialyseflüssigkeit entnommen und in den die verbrauchte Lösung nach der Dialyse zurückgeführt wird. Daher kommt der sicheren Aufbereitung des Systems eine besondere Bedeutung zu. Hierfür wird ein Aufbereitungsverfahren unter Verwendung von UV-Licht in Kombination mit einem chemischen Desinfektionsmittel angewendet. Ziel der hier beschriebenen Untersuchung war es, die Wirkungsbreite und Wirkungstiefe dieses Aufbereitungsverfahrens unter praxisnahen Phase-3-Bedingungen zu ermitteln. Dazu wurde das Gerät mit Mikroorganismen und Viren künstlich kontaminiert und die Wirkung der einzelnen Verfahrensschritte ermittelt. Im Gegensatz zu der üblichen Vorgehensweise praxisnaher Untersuchungen machen Aufbereitungsverfahren medizinischer Geräte unter Phase-3-Kriterien meist eine neuartige Arbeitsweise erforderlich – im Falle der hier vorgestellten Untersuchung sogar die Konstruktion eines speziellen Geräts zur Platzierung von Keimträgen im Genius-Tank. Im Ergebnis konnte gezeigt werden, dass bereits UV-Licht allein sowie in Kombination mit einem chemischen Desinfektionsmittel unter praxisnahen Bedingungen eine sichere Wirksamkeit gegen Bakterien (Pseudomonas aeruginosa) und bakterielle Sporen (Bacillus subtilis), Schimmelpilze (Aspergillus brasiliensis) und Viren (Murines Parvovirus) besitzt.

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