scholarly journals Non-muscle myosin IIA transports a Golgi glycosyltransferase to the endoplasmic reticulum by binding to its cytoplasmic tail

2012 ◽  
Vol 44 (7) ◽  
pp. 1153-1165 ◽  
Author(s):  
Armen Petrosyan ◽  
Mohamed F. Ali ◽  
Shailendra Kumar Verma ◽  
Helen Cheng ◽  
Pi-Wan Cheng
Keyword(s):  
Endoplasmic Reticulum ◽  
Cytoplasmic Tail ◽  
Myosin Iia ◽  
Muscle Myosin

scholarly journals Association between  4 integrin cytoplasmic tail and non-muscle myosin IIA regulates cell migration

2011 ◽  
Vol 124 (3) ◽  
pp. 483-492 ◽  
Author(s):  
L. A. R. Rosado ◽  
T. A. Horn ◽  
S. C. McGrath ◽  
R. J. Cotter ◽  
J. T. Yang
Keyword(s):  
Cell Migration ◽  
Cytoplasmic Tail ◽  
Myosin Iia ◽  
Muscle Myosin

scholarly journals Cleft lip with or without cleft palate: implication of the heavy chain of non-muscle myosin IIA

2007 ◽  
Vol 44 (6) ◽  
pp. 387-392 ◽  
Author(s):  
M. Martinelli ◽  
M. Di Stazio ◽  
L. Scapoli ◽  
J. Marchesini ◽  
F. Di Bari ◽  
...  
Keyword(s):  
Cleft Palate ◽  
Heavy Chain ◽  
Cleft Lip ◽  
Myosin Iia ◽  
Muscle Myosin

scholarly journals Author response: Presenilin/γ-secretase-dependent EphA3 processing mediates axon elongation through non-muscle myosin IIA

2019 ◽  
Author(s):  
Míriam Javier-Torrent ◽  
Sergi Marco ◽  
Daniel Rocandio ◽  
Maria Pons-Vizcarra ◽  
Peter W Janes ◽  
...  
Keyword(s):  
Author Response ◽  
Axon Elongation ◽  
Myosin Iia ◽  
Muscle Myosin

scholarly journals The tumor suppressor capability of p53 is dependent on non-muscle myosin IIA function in head and neck cancer

Oncotarget ◽  
2017 ◽  
Vol 8 (14) ◽  
pp. 22991-23007 ◽  
Author(s):  
Sonya D. Coaxum ◽  
Jessica Tiedeken ◽  
Elizabeth Garrett-Mayer ◽  
Jeffrey Myers ◽  
Steven A. Rosenzweig ◽  
...  
Keyword(s):  
Head And Neck Cancer ◽  
Tumor Suppressor ◽  
Head And Neck ◽  
Neck Cancer ◽  
Myosin Iia ◽  
Muscle Myosin

scholarly journals Deletion of both the Tyrosine-Based Endocytosis Signal and the Endoplasmic Reticulum Retrieval Signal in the Cytoplasmic Tail of Spike Protein Attenuates Porcine Epidemic Diarrhea Virus in Pigs

2018 ◽  
Vol 93 (2) ◽  
Author(s):  
Yixuan Hou ◽  
Tea Meulia ◽  
Xiang Gao ◽  
Linda J. Saif ◽  
Qiuhong Wang
Keyword(s):  
Endoplasmic Reticulum ◽  
Vero Cell ◽  
Porcine Epidemic Diarrhea Virus ◽  
Cytoplasmic Tail ◽  
S Protein ◽  
Diarrhea Virus ◽  
Neonatal Piglets ◽  
Porcine Epidemic Diarrhea ◽  
Intracellular Sorting ◽  
S Proteins

ABSTRACTPorcine epidemic diarrhea virus (PEDV) causes high mortality in neonatal piglets. The PEDV spike (S) protein contains two intracellular sorting motifs, YxxΦ (tyrosine-based motif YEVF or YEAF) and KVHVQ at the cytoplasmic tail, yet their functions have not been fully elucidated. Some Vero cell-adapted and/or attenuated PEDV variants contain ablations in these two motifs. We hypothesized that these motifs contribute to viral pathogenicity. By transiently expressing PEDV S proteins with mutations in the motifs, we confirmed that the motif KVHVQ is involved in retention of the S proteins in the endoplasmic reticulum (ER)-Golgi intermediate compartment (ERGIC). In addition, we showed that the YxxΦ motif triggers endocytosis of S proteins. These two motifs synergistically regulate the level of S expressed on the cell surface. To investigate their role in viral pathogenicity, we generated three recombinant PEDVs by introducing deletions or a mutation in the two motifs of the infectious clone of PEDV PC22A strain (icPC22A): (i) icΔ10aa (ΔYxxΦEKVHVQ), (ii) icΔ5aa (ΔKVHVQ), and (iii) icYA (Y1378A, to an inactivated motif, AEVF). Infection of Vero cells with icΔ10aa resulted in larger syncytia and more virions, with reduced numbers of S protein projections on the surface compared with icPC22A. Furthermore, we orally inoculated five groups of 5-day-old gnotobiotic piglets with the three mutants, icPC22A, or a mock treatment. Mutant icΔ10aa caused less severe diarrhea rate and significantly milder intestinal lesions than icPC22A, icΔ5aa, and icYA. These data suggest that the deletion of both motifs can reduce the virulence of PEDV in piglets.IMPORTANCEMany coronaviruses (CoVs) possess conserved motifs YxxΦ and/or KxHxx/KKxx in the cytoplasmic tail of the S protein. The KxHxx/KKxx motif has been identified as the ER retrieval signal, but the function of the YxxΦ motif in the intracellular sorting of CoV S proteins remains controversial. In this study, we showed that the YxxΦ of PEDV S protein is an endocytosis signal. Furthermore, using reverse genetics technology, we evaluated its role in PEDV pathogenicity in neonatal piglets. Our results explain one attenuation mechanism of Vero cell-adapted PEDV variants lacking functional YxxΦ and KVHVQ motifs. Knowledge from this study may aid in the design of efficacious live attenuated vaccines against PEDV, as well as other CoVs bearing the same motif in their S protein.

scholarly journals MYH9: Structure, functions and role of non-muscle myosin IIA in human disease

Gene ◽  
2018 ◽  
Vol 664 ◽  
pp. 152-167 ◽  
Author(s):  
Alessandro Pecci ◽  
Xuefei Ma ◽  
Anna Savoia ◽  
Robert S. Adelstein
Keyword(s):  
Human Disease ◽  
Structure Functions ◽  
Myosin Iia ◽  
Muscle Myosin

Abstract 4205: The role of non-muscle myosin IIA in HER2+ breast cancers

2020 ◽  
Author(s):  
Samar M. Alanazi ◽  
Rosalin Mishra ◽  
Long Yuan ◽  
Hima Patel ◽  
Joan Garrett
Keyword(s):  
Breast Cancers ◽  
Myosin Iia ◽  
Muscle Myosin
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