Oxidized low density lipoprotein-induced senescence of retinal pigment epithelial cells is followed by outer blood–retinal barrier dysfunction

2012 ◽  
Vol 44 (5) ◽  
pp. 808-814 ◽  
Author(s):  
Jin Hyoung Kim ◽  
Sung-Joon Lee ◽  
Kyu-Won Kim ◽  
Young Suk Yu ◽  
Jeong Hun Kim
Keyword(s):  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Retinal Pigment Epithelial Cells ◽  
Oxidized Low Density Lipoprotein ◽  
Barrier Dysfunction ◽  
Blood Retinal Barrier ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

scholarly journals Eucalyptol Blocks Diabetes-Associated Disruption of Tight Junction and Blood Retinal Barrier in Retinal Pigment Epithelial Cells and Diabetic Eyes

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 413-413
Author(s):  
Dongyeon Kim ◽  
Min-kyung Kang ◽  
Young-Hee Kang
Keyword(s):  
Epithelial Cells ◽  
Tight Junction ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Tight Junction Proteins ◽  
Blood Retinal Barrier ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells ◽  
Junction Proteins

Abstract Objectives Diabetes-associated retinal impairment has been implicated in diabetic retinopathy. Chronic hyperglycemia leads to disruption of tight junction and breakdown of blood retinal barrier. Eucalyptol is a natural organic essential oil and a monoterpenoid present in eucalyptus oil with anti-inflammatory, anti-diabetic and antioxidant properties. Methods Primary human retinal pigment epithelial cells (HRPEC) were cultured in media containing 33 mM glucose for 4 days in the presence of 1–20 μM eucalyptol. The in vivo animal study employed db/db mice orally administrated with 10 mg/kg eucalyptol. Cell lysates and mouse eye tissue extracts were prepared for Western blotting, in which antibodies of ZO-1, occludin, matrix metalloproteinase (MMP)-2 and MMP-9 were used. Results Eucalyptol enhanced epithelial induction of the tight junction proteins of ZO-1 and occludin reduced by glucose loading. Consistently, oral administration of eucalyptol to db/db mice augmented the eye tissue levels of these tight junction proteins. In addition, the induction of MMP-2 and MMP-9 involved in the degradation of extracellular matrix, was elevated by exposure of glucose to HRPEC, which was encumbered by eucalyptol in a dose-dependent manner. Conclusions These results demonstrated that eucalyptol maintained transepithelial cells integrity and blood retinal barrier in diabetic eyes. Therefore, eucalyptol may be a potent retinoprotective agent combating diabetes-associated retinal malfunction. Funding Sources This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (2017R1A6A3A04011473).

scholarly journals Eucalyptol Inhibits Amyloid-β-Induced Barrier Dysfunction in Glucose-Exposed Retinal Pigment Epithelial Cells and Diabetic Eyes

Antioxidants ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1000
Author(s):  
Dong Yeon Kim ◽  
Min-Kyung Kang ◽  
Eun-Jung Lee ◽  
Yun-Ho Kim ◽  
Hyeongjoo Oh ◽  
...  
Keyword(s):  
Tight Junction ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Amyloid Β ◽  
Diabetic Mouse ◽  
Retinal Pigment Epithelial Cells ◽  
Barrier Dysfunction ◽  
Blood Retinal Barrier ◽  
Rpe Cells ◽  
Pigment Epithelial

Hyperglycemia elicits tight junction disruption and blood-retinal barrier breakdown, resulting in diabetes-associated vison loss. Eucalyptol is a natural compound found in eucalyptus oil with diverse bioactivities. This study evaluated that eucalyptol ameliorated tight junctions and retinal barrier function in glucose/amyloid-β (Aβ)-exposed human retinal pigment epithelial (RPE) cells and in db/db mouse eyes. RPE cells were cultured in media containing 33 mM glucose or 5 μM Aβ for 4 days in the presence of 1–20 μM eucalyptol. The in vivo animal study employed db/db mice orally administrated with 10 mg/kg eucalyptol. Nontoxic eucalyptol inhibited the Aβ induction in glucose-loaded RPE cells and diabetic mouse eyes. Eucalyptol reversed the induction of tight junction-associated proteins of ZO-1, occludin-1 and matrix metalloproteinases in glucose- or Aβ-exposed RPE cells and in diabetic eyes, accompanying inhibition of RPE detachment from Bruch’s membrane. Adding eucalyptol to glucose- or Aβ-loaded RPE cells, and diabetic mouse eyes reciprocally reversed induction/activation of apoptosis-related bcl-2, bax, cytochrome C/Apaf-1 and caspases. Eucalyptol attenuated the generation of reactive oxygen species and the induction of receptor for advanced glycation end products in Aβ-exposed RPE cells and diabetic eyes. Eucalyptol may ameliorate RPE barrier dysfunction in diabetic eyes through counteracting Aβ-mediated oxidative stress-induced RPE cell apoptosis.

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