Interaction between microglia and retinal pigment epithelial cells determines the integrity of outer blood-retinal barrier in diabetic retinopathy

Glia ◽  
2018 ◽  
Vol 67 (2) ◽  
pp. 321-331 ◽  
Author(s):  
Dong Hyun Jo ◽  
Jang-Hyuk Yun ◽  
Chang Sik Cho ◽  
Jin Hyoung Kim ◽  
Jeong Hun Kim ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Blood Retinal Barrier ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells

scholarly journals Eucalyptol Blocks Diabetes-Associated Disruption of Tight Junction and Blood Retinal Barrier in Retinal Pigment Epithelial Cells and Diabetic Eyes

2020 ◽  
Vol 4 (Supplement_2) ◽  
pp. 413-413
Author(s):  
Dongyeon Kim ◽  
Min-kyung Kang ◽  
Young-Hee Kang
Keyword(s):  
Epithelial Cells ◽  
Tight Junction ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Tight Junction Proteins ◽  
Blood Retinal Barrier ◽  
Pigment Epithelial ◽  
Pigment Epithelial Cells ◽  
Junction Proteins

Abstract Objectives Diabetes-associated retinal impairment has been implicated in diabetic retinopathy. Chronic hyperglycemia leads to disruption of tight junction and breakdown of blood retinal barrier. Eucalyptol is a natural organic essential oil and a monoterpenoid present in eucalyptus oil with anti-inflammatory, anti-diabetic and antioxidant properties. Methods Primary human retinal pigment epithelial cells (HRPEC) were cultured in media containing 33 mM glucose for 4 days in the presence of 1–20 μM eucalyptol. The in vivo animal study employed db/db mice orally administrated with 10 mg/kg eucalyptol. Cell lysates and mouse eye tissue extracts were prepared for Western blotting, in which antibodies of ZO-1, occludin, matrix metalloproteinase (MMP)-2 and MMP-9 were used. Results Eucalyptol enhanced epithelial induction of the tight junction proteins of ZO-1 and occludin reduced by glucose loading. Consistently, oral administration of eucalyptol to db/db mice augmented the eye tissue levels of these tight junction proteins. In addition, the induction of MMP-2 and MMP-9 involved in the degradation of extracellular matrix, was elevated by exposure of glucose to HRPEC, which was encumbered by eucalyptol in a dose-dependent manner. Conclusions These results demonstrated that eucalyptol maintained transepithelial cells integrity and blood retinal barrier in diabetic eyes. Therefore, eucalyptol may be a potent retinoprotective agent combating diabetes-associated retinal malfunction. Funding Sources This work was supported by the National Research Foundation of Korea (NRF) grants funded by the Korea government (2017R1A6A3A04011473).

scholarly journals GPR109A as an Anti-Inflammatory Receptor in Retinal Pigment Epithelial Cells and Its Relevance to Diabetic Retinopathy

2012 ◽  
Vol 53 (4) ◽  
pp. 2208 ◽  
Author(s):  
Deeksha Gambhir ◽  
Sudha Ananth ◽  
Rajalakshmi Veeranan-Karmegam ◽  
Selvakumar Elangovan ◽  
Shanterian Hester ◽  
...  
Keyword(s):  
Diabetic Retinopathy ◽  
Epithelial Cells ◽  
Retinal Pigment Epithelial ◽  
Retinal Pigment ◽  
Retinal Pigment Epithelial Cells ◽  
Pigment Epithelial ◽  
Anti Inflammatory ◽  
Pigment Epithelial Cells
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