scholarly journals How good is a PCR efficiency estimate: Recommendations for precise and robust qPCR efficiency assessments

2015 ◽  
Vol 3 ◽  
pp. 9-16 ◽  
Author(s):  
David Svec ◽  
Ales Tichopad ◽  
Vendula Novosadova ◽  
Michael W. Pfaffl ◽  
Mikael Kubista
Keyword(s):  
Pcr Efficiency ◽  
Efficiency Estimate

Enhancement of PCR efficiency using mono- and disaccharides

2020 ◽  
Vol 606 ◽  
pp. 113858
Author(s):  
Assol R. Sakhabutdinova ◽  
Alexey V. Chemeris ◽  
Ravil R. Garafutdinov
Keyword(s):  
Pcr Efficiency

scholarly journals Data on individual PCR efficiency values as quality control for circulating miRNAs

Data in Brief ◽  
2015 ◽  
Vol 5 ◽  
pp. 321-326 ◽  
Author(s):  
Anna Brunet-Vega ◽  
Carles Pericay ◽  
María Elisa Quílez ◽  
María José Ramírez-Lázaro ◽  
Xavier Calvet ◽  
...  
Keyword(s):  
Quality Control ◽  
Circulating Mirnas ◽  
Pcr Efficiency

scholarly journals Enhancing multiplex PCR efficiency using Hot Start dNTPs

BioTechniques ◽  
2009 ◽  
Vol 47 (5) ◽  
pp. 972-973 ◽  
Author(s):  
Tony Le ◽  
Elena Hidalgo Ashrafi ◽  
Natasha Paul
Keyword(s):  
Multiplex Pcr ◽  
Pcr Efficiency ◽  
Hot Start

A BSA coated NOA81 PCR chip for gene amplification

2016 ◽  
Vol 8 (12) ◽  
pp. 2584-2591 ◽  
Author(s):  
Kuiwei Qin ◽  
Xuefei Lv ◽  
Qiaorui Xing ◽  
Rui Li ◽  
Yulin Deng
Keyword(s):  
Gene Amplification ◽  
Pcr Efficiency ◽  
Coating Method

We fabricated a NOA81 PCR chip (NP-chip) and first proposed a simple BSA coating method to successfully overcome the low PCR efficiency induced by the inhibition of the NP-chip which seriously impacted its application in the area of gene amplification.

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