Purification and functional characterization of a novel tyrosinase (diphenolase) inhibitory peptides prepared from Solunum tuberosum peels protein via enzymatic hydrolysis

2019 ◽  
Vol 17 ◽  
pp. 331-338 ◽  
Author(s):  
Sanaa T. El-Sayed ◽  
Rehab A. Al- Azzouny ◽  
Ola S. Ali
Keyword(s):  
Enzymatic Hydrolysis ◽  
Functional Characterization ◽  
Inhibitory Peptides

Generation and functional characterization of a BCL10-inhibitory peptide that represses NF-κB activation

2009 ◽  
Vol 422 (3) ◽  
pp. 553-561 ◽  
Author(s):  
Daniela Marasco ◽  
Romania Stilo ◽  
Annamaria Sandomenico ◽  
Simona Maria Monti ◽  
Barbara Tizzano ◽  
...  
Keyword(s):  
Functional Characterization ◽  
Nuclear Factor Κb ◽  
Lymphoid Cells ◽  
Amino Acid Residues ◽  
Inhibitory Peptide ◽  
Inhibitory Peptides ◽  
Cell Assays ◽  
Self Association ◽  
Competition Assays

The molecular complex containing BCL10 and CARMA [CARD (caspase recruitment domain)-containing MAGUK (membrane-associated guanylate kinase)] proteins has recently been identified as a key component in the signal transduction pathways that regulate activation of the transcription factor NF-κB (nuclear factor κB) in lymphoid and non-lymphoid cells. Assembly of complexes containing BCL10 and CARMA proteins relies on homophilic interactions established between the CARDs of these proteins. In order to identify BCL10-inhibitory peptides, we have established a method of assaying peptides derived from the CARD of BCL10 in binding competition assays of CARD–CARD self-association. By this procedure, a short peptide corresponding to amino acid residues 91–98 of BCL10 has been selected as an effective inhibitor of protein self-association. When tested in cell assays for its capacity to block NF-κB activation, this peptide represses activation of NF-κB mediated by BCL10, CARMA3 and PMA/ionomycin stimulation. Collectively, these results indicate that residues 91–98 of BCL10 are involved in BCL10 self-association and also participate in the interaction with external partners. We also show that blocking of the CARD of BCL10 may potentially be used for the treatment of pathological conditions associated with inappropriate NF-κB activation.

scholarly journals Hydrolysis Process Optimization and Functional Characterization of Yak Skin Gelatin Hydrolysates

2019 ◽  
Vol 2019 ◽  
pp. 1-11
Author(s):  
Hui Yang ◽  
Yanting Xue ◽  
Jiaheng Liu ◽  
Shunyi Song ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Functional Characterization ◽  
Meat Production ◽  
Degree Of Hydrolysis ◽  
Foaming Properties ◽  
The Tibetan Plateau ◽  
Hydrolysis Process ◽  
Potential Applications ◽  
Central Composite

Yak (Bos grunniens) is an animal mainly living on the Tibetan Plateau. Yak skin is a valuable resource that is wasted in the meat production process. This study aimed to prepare yak skin gelatin hydrolysates (YSGH) from yak skin through enzymatic hydrolysis and investigate functional characterization of YSGH. We showed that trypsin was more effective than neutrase, papain, and pepsin in increasing the degree of hydrolysis (DH) of YSGH. The conditions of enzymatic hydrolysis were optimized using central composite design (CCD) and response surface method (RSM), and the highest DH value of 31.96% was obtained. We then analyzed the amino acid compositions and molecular weight distribution of peptides in YSGH. The obtained YSGH exhibited certain antioxidant activity and excellent ACE-inhibitory activity (IC50 = 0.991 mg/mL). In addition, the solubility (98.79%), emulsification, and foaming properties of YSGH developed here were also evaluated. With these physicochemical and biological functions, YSGH had potential applications in food, pharmaceuticals, and cosmetics as an ingredient.

Purification and Characterization of Nitric Oxide Inhibitory Peptides fromActinopyga lecanoraThrough Enzymatic Hydrolysis

2016 ◽  
Vol 30 (4) ◽  
pp. 263-277 ◽  
Author(s):  
Raheleh Ghanbari ◽  
Afshin Ebrahimpour ◽  
Mohammad Zarei ◽  
Amin Ismail ◽  
Azizah Abdul-Hamid ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Enzymatic Hydrolysis ◽  
Purification And Characterization ◽  
Inhibitory Peptides

Functional characterization of human brown adipose tissue metabolism

2020 ◽  
Vol 477 (7) ◽  
pp. 1261-1286 ◽  
Author(s):  
Marie Anne Richard ◽  
Hannah Pallubinsky ◽  
Denis P. Blondin
Keyword(s):  
Adipose Tissue ◽  
Brown Adipose Tissue ◽  
Functional Characterization ◽  
Human Infants ◽  
Positron Emission ◽  
Brown Adipose ◽  
Treatment Of Obesity ◽  
And Function

Brown adipose tissue (BAT) has long been described according to its histological features as a multilocular, lipid-containing tissue, light brown in color, that is also responsive to the cold and found especially in hibernating mammals and human infants. Its presence in both hibernators and human infants, combined with its function as a heat-generating organ, raised many questions about its role in humans. Early characterizations of the tissue in humans focused on its progressive atrophy with age and its apparent importance for cold-exposed workers. However, the use of positron emission tomography (PET) with the glucose tracer [18F]fluorodeoxyglucose ([18F]FDG) made it possible to begin characterizing the possible function of BAT in adult humans, and whether it could play a role in the prevention or treatment of obesity and type 2 diabetes (T2D). This review focuses on the in vivo functional characterization of human BAT, the methodological approaches applied to examine these features and addresses critical gaps that remain in moving the field forward. Specifically, we describe the anatomical and biomolecular features of human BAT, the modalities and applications of non-invasive tools such as PET and magnetic resonance imaging coupled with spectroscopy (MRI/MRS) to study BAT morphology and function in vivo, and finally describe the functional characteristics of human BAT that have only been possible through the development and application of such tools.

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