Optimization of production conditions for xylanase production by newly isolated strain Aspergillus niger through solid state fermentation of oil palm empty fruit bunches

2017 ◽  
Vol 11 ◽  
pp. 239-247 ◽  
Author(s):  
A. Kamoldeen Ajijolakewu ◽  
Cheu Peng Leh ◽  
Wan Nadiah Wan Abdullah ◽  
Chee keong Lee
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Oil Palm ◽  
Xylanase Production ◽  
Empty Fruit Bunches ◽  
Production Conditions

scholarly journals PENGARUH AERASI PADA FERMENTASI PADAT TANDAN KOSONG KELAPA SAWIT OLEH Aspergillus niger TERHADAP PRODUKSI GULA SEDERHANA

2016 ◽  
Vol 5 (3) ◽  
pp. 12-16
Author(s):  
Himmatun Wisda ◽  
Wahyudi Budi Sediawan ◽  
Sarto
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Solid State Fermentation ◽  
Oil Palm ◽  
Reaction Kinetic ◽  
Cellulosic Biomass ◽  
Empty Fruit Bunch ◽  
Simple Sugar

The effects of aeration on production of simple sugars by Aspergillus niger were investigated using oil palm empty fruit bunch (OPEFB) by solid state fermentation using Aspergillus niger. Simple sugars can further be  fermented to produce bioethanol. OPEFB is renewable cellulosic biomass and largely available in Indonesia. Before being fermented, OPEFB needs to be pretreated. It was done by NaOH 1% solution at 150 ºC for 2 hours. The concentrations simple sugars produced by hydrolysis were analyzed by Dinitrosalicylic acid (DNS). It turned out that the highest concentration of sugars was to be 9,102 g/L, and it was obtained on the 3rd day of fermentation. Some parts of the sugars produced were consumed by Aspergillus niger itself for growth and maintenance. The experiment showed that aeration increased the simple sugars concentration. Modelling of reaction kinetic assumed to hydrolisis process and simple sugar consumed. Simply modelling can describe in quantitative fermentation of OPEFB. Moreover, get parameters value at equation of reaction kinetic.

scholarly journals Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design

2011 ◽  
Vol 10 (46) ◽  
pp. 9402-9409 ◽  
Author(s):  
Zauri Abdul Wahid Manisya ◽  
Salleh Madihah ◽  
Yusof Faridah ◽  
Ismail Abdul Karim Mohammed ◽  
Zahangir Alam Md
Keyword(s):  
Solid State ◽  
Trichoderma Reesei ◽  
Solid State Fermentation ◽  
Oil Palm ◽  
Factors Affecting ◽  
Empty Fruit Bunches ◽  
Burman Design ◽  
C 30 ◽  
Plackett Burman Design

scholarly journals Optimisation of solid-state fermentation of Aspergillus niger JL-15 for xylanase production and xylooligosaccharides preparation

2011 ◽  
Vol 29 (No. 5) ◽  
pp. 557-567 ◽  
Author(s):  
X.-J. Dai ◽  
M.-Q. Liu ◽  
H.-X. Jin ◽  
M.-Y. Jing
Keyword(s):  
Solid State ◽  
Aspergillus Niger ◽  
Moisture Content ◽  
Solid State Fermentation ◽  
Xylanase Activity ◽  
Orange Peel ◽  
Basic Medium ◽  
Relative Molecular Mass ◽  
Xylanase Production ◽  
Fermentation Time

The production of xylanase (XylA) by Aspergillus niger JL-15 in solid-state fermentation (SSF) on orange peel was optimised by the response surface methodology (RSM). The results revealed that four factors had significant effects on the XylA production (P &lt; 0.05), that is the concentrations of the added glycerin and ammonium sulfate, the moisture content, and fermentation time. Exploying orange peel as the solid substrate, maximum xylanase activity (917.7&nbsp;U/g dry fermentation product) was obtained at 4.2% glycerin, 3.1% (NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>, 61% moisture content, and 73.4-h fermentation, this activity being close to the predicted one and 3.2 times higher than that of the basic medium (218.5&nbsp;U/g). Optimum temperature and pH for XylA activity were 55&deg;C and pH 5.0, respectively. SDS-PAGE analysis showed that the relative molecular mass of XylA was about 30.0 kDa. XylA exhibited K<sub>m</sub> and V<sub>max </sub>values of 9.24 mg/ml and 54.05&nbsp;&mu;mol/min/ml, respectively. XylA liberated mainly xylotriose from birchwood xylan and wheat bran, respectively. XylA was an endo-acting xylanase with transglycosylation activity, with the ability to hydrolyse, xylobiose, xylotriose, xylotetraose, xylopentaose, and xylohexaose.

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