Comprehensive studies on optimization of cellulase and xylanase production by a local indigenous fungus strain via solid state fermentation using oil palm frond as substrate

2019 ◽  
Vol 35 (3) ◽  
pp. e2781 ◽  
Author(s):  
Wan Yi Tai ◽  
Joo Shun Tan ◽  
Vuanghao Lim ◽  
Chee Keong Lee
Author(s):  
N. A. Nata ◽  
F. Mohd Said ◽  
S. Md Shaarani@Md Nawi ◽  
N. Harun

Lovastatin is a potent drug for lowering the blood cholesterol. It is a competitive inhibitor of 3-hyroxy3-methyl glutaryl coenzyme A (HMG-CoA) reductase, which is a key enzyme in the cholesterol production pathway. Lovastatin increases the good cholesterol or high-density lipoproteins to prevent the formation of plaque inside the blood vessels. This study aims to develop a process model of lovastatin production, produced by Monascus purpureus under solid-state fermentation using oil palm frond. SuperPro Design V9.5 software was used to develop and simulate the process model. Three parameters which are initial moisture content, composition of peptone and potassium, were varied to investigate their effects on lovastatin production. The optimum condition simulated using the process model at pH 7 with 60% initial moisture content, 0.0075 kg/hr of potassium, and 0.0075 kg/hr of peptone was able to produce 0.0288 kg/kg of lovastatin. The simulated results show good agreement with experimental work, with low percentage error of 5.77%, and provide a good approximation on the production of lovastatin under various process operating conditions.


2021 ◽  
Vol 1025 ◽  
pp. 150-156
Author(s):  
Nur Fathin Shamirah Daud ◽  
Farhan Mohd Said ◽  
Nur Hidayah Mat Yasin ◽  
Mior Ahmad Khusairi Mohd Zahari

Extensive study available on Monascus in solid-state fermentation (SSF), however, optimization study of Monascus sp. cultivated in stirred drum bioreactor is insufficiently reported. In this study, the Box–Behnken design (BBD) was employed for the analysis of the simultaneous effect of initial moisture content, aeration rate and peptone concentration to the red pigments production of Monascus purpureus FTC 5357 by using oil palm frond (OPF) in 5 L stirred drum bioreactor. A three-parameters, three-level BBD was used for the optimization. Based on the ANOVA analysis performed, initial moisture content, aeration rate and peptone concentration contributed significantly to the red pigments production. The optimal fermentation conditions resulted were initial moisture content; 70 % w/w, aeration rate; 1.30 vvm and peptone concentration; 4.40 % w/w. Under these conditions, the red pigments production were obtained to be 18.59 Au/g.d. The red pigments produced through SSF using OPF as a substrate by Monascus purpureus FTC 5357 has a great potential to be utilized as a source of pigment for food in future.


3 Biotech ◽  
2018 ◽  
Vol 8 (5) ◽  
Author(s):  
Mohamed Roslan Mohamad Ikubar ◽  
Musaalbakri Abdul Manan ◽  
Madihah Md. Salleh ◽  
Adibah Yahya

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