Construction and characterization of a pseudo-immune human antibody library using yeast surface display

2006 ◽  
Vol 346 (3) ◽  
pp. 896-903 ◽  
Author(s):  
Hae-Won Lee ◽  
Seung-Hyun Lee ◽  
Kyung-Jin Park ◽  
Jeong-Sun Kim ◽  
Myung-Hee Kwon ◽  
...  
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Human Antibody ◽  
Antibody Library ◽  
Yeast Surface

scholarly journals Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality

2021 ◽  
Author(s):  
Jimmy D Gollihar ◽  
Jason S McLellan ◽  
Daniel R Boutz ◽  
Jule Goike ◽  
Andrew Horton ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Surface Display ◽  
Functional Characterization ◽  
Yeast Surface Display ◽  
Spike Protein ◽  
Emerging Pathogens ◽  
Effective Interventions ◽  
Yeast Surface

The ongoing evolution of SARS-CoV-2 into more easily transmissible and infectious variants has sparked concern over the continued effectiveness of existing therapeutic antibodies and vaccines. Hence, together with increased genomic surveillance, methods to rapidly develop and assess effective interventions are critically needed. Here we report the discovery of SARS-CoV-2 neutralizing antibodies isolated from COVID-19 patients using a high-throughput platform. Antibodies were identified from unpaired donor B-cell and serum repertoires using yeast surface display, proteomics, and public light chain screening. Cryo-EM and functional characterization of the antibodies identified N3-1, an antibody that binds avidly (Kd,app = 68 pM) to the receptor binding domain (RBD) of the spike protein and robustly neutralizes the virus in vitro. This antibody likely binds all three RBDs of the trimeric spike protein with a single IgG. Importantly, N3-1 equivalently binds spike proteins from emerging SARS-CoV-2 variants of concern, neutralizes UK variant B.1.1.7, and binds SARS-CoV spike with nanomolar affinity. Taken together, the strategies described herein will prove broadly applicable in interrogating adaptive immunity and developing rapid response biological countermeasures to emerging pathogens.

Construction and characterization of a thermostable whole-cell chitinolytic enzyme using yeast surface display

2014 ◽  
Vol 30 (10) ◽  
pp. 2577-2585 ◽  
Author(s):  
Xiaobo Li ◽  
Xiaobao Jin ◽  
Xuemei Lu ◽  
Fujiang Chu ◽  
Juan Shen ◽  
...  
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Chitinolytic Enzyme ◽  
Whole Cell ◽  
Yeast Surface

Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens

2014 ◽  
Vol 61 (3) ◽  
pp. 230-239 ◽  
Author(s):  
Milica Popovic ◽  
Radivoje Prodanovic ◽  
Raluca Ostafe ◽  
Stefan Schillberg ◽  
Rainer Fischer ◽  
...  
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Food Allergens ◽  
Immunological Characterization ◽  
Yeast Surface

Functional expression, production, and biochemical characterization of a laccase using yeast surface display technology

2016 ◽  
Vol 120 (12) ◽  
pp. 1609-1622 ◽  
Author(s):  
Brandt Bertrand ◽  
María R. Trejo-Hernández ◽  
Daniel Morales-Guzmán ◽  
Luis Caspeta ◽  
Ramón Suárez Rodríguez ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Surface Display ◽  
Functional Expression ◽  
Yeast Surface Display ◽  
Display Technology ◽  
Yeast Surface

Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases

2013 ◽  
Vol 89 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Marija Blazic ◽  
Gordana Kovacevic ◽  
Olivera Prodanovic ◽  
Raluca Ostafe ◽  
Marija Gavrovic-Jankulovic ◽  
...  
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Wild Type ◽  
Purification And Characterization ◽  
Yeast Surface

scholarly journals Displaying Lipase B from Candida antarctica in Pichia pastoris Using the Yeast Surface Display Approach: Prospection of a New Anchor and Characterization of the Whole Cell Biocatalyst

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0141454 ◽  
Author(s):  
Marcelo Victor Holanda Moura ◽  
Giulia Pontes da Silva ◽  
Antônio Carlos de Oliveira Machado ◽  
Fernando Araripe Gonçalves Torres ◽  
Denise Maria Guimarães Freire ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Surface Display ◽  
Candida Antarctica ◽  
Yeast Surface Display ◽  
Lipase B ◽  
Whole Cell ◽  
Whole Cell Biocatalyst ◽  
Yeast Surface

scholarly journals Identification and Characterization of Ixodes scapularis Antigens That Elicit Tick Immunity Using Yeast Surface Display

PLoS ONE ◽  
2011 ◽  
Vol 6 (1) ◽  
pp. e15926 ◽  
Author(s):  
Tim J. Schuijt ◽  
Sukanya Narasimhan ◽  
Sirlei Daffre ◽  
Kathleen DePonte ◽  
Joppe W. R. Hovius ◽  
...  
Keyword(s):  
Ixodes Scapularis ◽  
Surface Display ◽  
Yeast Surface Display ◽  
Yeast Surface ◽  
Identification And Characterization
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