Functional expression, production, and biochemical characterization of a laccase using yeast surface display technology

2016 ◽  
Vol 120 (12) ◽  
pp. 1609-1622 ◽  
Author(s):  
Brandt Bertrand ◽  
María R. Trejo-Hernández ◽  
Daniel Morales-Guzmán ◽  
Luis Caspeta ◽  
Ramón Suárez Rodríguez ◽  
...  
Keyword(s):  
Biochemical Characterization ◽  
Surface Display ◽  
Functional Expression ◽  
Yeast Surface Display ◽  
Display Technology ◽  
Yeast Surface

scholarly journals Synthetic repertoires derived from convalescent COVID-19 patients enable discovery of SARS-CoV-2 neutralizing antibodies and a novel quaternary binding modality

2021 ◽  
Author(s):  
Jimmy D Gollihar ◽  
Jason S McLellan ◽  
Daniel R Boutz ◽  
Jule Goike ◽  
Andrew Horton ◽  
...  
Keyword(s):  
Neutralizing Antibodies ◽  
Surface Display ◽  
Functional Characterization ◽  
Yeast Surface Display ◽  
Spike Protein ◽  
Emerging Pathogens ◽  
Effective Interventions ◽  
Yeast Surface

The ongoing evolution of SARS-CoV-2 into more easily transmissible and infectious variants has sparked concern over the continued effectiveness of existing therapeutic antibodies and vaccines. Hence, together with increased genomic surveillance, methods to rapidly develop and assess effective interventions are critically needed. Here we report the discovery of SARS-CoV-2 neutralizing antibodies isolated from COVID-19 patients using a high-throughput platform. Antibodies were identified from unpaired donor B-cell and serum repertoires using yeast surface display, proteomics, and public light chain screening. Cryo-EM and functional characterization of the antibodies identified N3-1, an antibody that binds avidly (Kd,app = 68 pM) to the receptor binding domain (RBD) of the spike protein and robustly neutralizes the virus in vitro. This antibody likely binds all three RBDs of the trimeric spike protein with a single IgG. Importantly, N3-1 equivalently binds spike proteins from emerging SARS-CoV-2 variants of concern, neutralizes UK variant B.1.1.7, and binds SARS-CoV spike with nanomolar affinity. Taken together, the strategies described herein will prove broadly applicable in interrogating adaptive immunity and developing rapid response biological countermeasures to emerging pathogens.

Construction and characterization of a thermostable whole-cell chitinolytic enzyme using yeast surface display

2014 ◽  
Vol 30 (10) ◽  
pp. 2577-2585 ◽  
Author(s):  
Xiaobo Li ◽  
Xiaobao Jin ◽  
Xuemei Lu ◽  
Fujiang Chu ◽  
Juan Shen ◽  
...  
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Chitinolytic Enzyme ◽  
Whole Cell ◽  
Yeast Surface

Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens

2014 ◽  
Vol 61 (3) ◽  
pp. 230-239 ◽  
Author(s):  
Milica Popovic ◽  
Radivoje Prodanovic ◽  
Raluca Ostafe ◽  
Stefan Schillberg ◽  
Rainer Fischer ◽  
...  
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Food Allergens ◽  
Immunological Characterization ◽  
Yeast Surface

scholarly journals A Decade of Yeast Surface Display Technology: Where Are We Now?

2008 ◽  
Vol 11 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Eric Shusta ◽  
Lauren Pepper ◽  
Yong Cho ◽  
Eric Boder
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Display Technology ◽  
Yeast Surface

Yeast surface display for the expression, purification and characterization of wild-type and B11 mutant glucose oxidases

2013 ◽  
Vol 89 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Marija Blazic ◽  
Gordana Kovacevic ◽  
Olivera Prodanovic ◽  
Raluca Ostafe ◽  
Marija Gavrovic-Jankulovic ◽  
...  
Keyword(s):  
Surface Display ◽  
Yeast Surface Display ◽  
Wild Type ◽  
Purification And Characterization ◽  
Yeast Surface

scholarly journals Converting a broad matrix metalloproteinase family inhibitor into a specific inhibitor of MMP-9 and MMP-14

2017 ◽  
Author(s):  
Jason Shirian ◽  
Valeria Arkadash ◽  
Itay Cohen ◽  
Tamila Sapir ◽  
Evette S. Radisky ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Surface Display ◽  
Specific Inhibitor ◽  
Computational Design ◽  
Yeast Surface Display ◽  
Physiological Conditions ◽  
Mmp Inhibitor ◽  
Single Target ◽  
Display Technology ◽  
Yeast Surface

AbstractMMP-14 and MMP-9 are two well established cancer targets for which no specific clinically relevant inhibitor is available. Using a powerful combination of computational design and yeast surface display technology, we engineered such an inhibitor starting from a non-specific MMP inhibitor, N-TIMP2. The engineered purified N-TIMP2 variants showed enhanced specificity towards MMP-14 and MMP-9 relative to a panel of off-target MMPs. MMP-specific N-TIMP2 sequence signatures were obtained that could be understood from the structural perspective of MMP/N-TIMP2 interactions. Our MMP-9 inhibitor exhibited 1000-fold preference for MMP-9 vs. MMP-14, which is likely to translate into significant differences under physiological conditions. Our results provide new insights regarding evolution of promiscuous proteins and optimization strategies for design of inhibitors with single-target specificities.

scholarly journals Molecular evolution of peptides by yeast surface display technology

MedChemComm ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 1569-1580 ◽  
Author(s):  
Sara Linciano ◽  
Stefano Pluda ◽  
Arianna Bacchin ◽  
Alessandro Angelini
Keyword(s):  
Molecular Evolution ◽  
Detailed Analysis ◽  
Surface Display ◽  
Yeast Surface Display ◽  
Display Technology ◽  
Yeast Surface

This review provides a detailed analysis of the diverse genetically encoded peptides that have been evolved by using yeast surface display technology.

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