Palmitic acid-induced activation of human T-lymphocytes and aortic endothelial cells with production of insulin receptors, reactive oxygen species, cytokines, and lipid peroxidation

2006 ◽  
Vol 346 (3) ◽  
pp. 721-726 ◽  
Author(s):  
Frankie B. Stentz ◽  
Abbas E. Kitabchi
Keyword(s):  
Reactive Oxygen Species ◽  
Lipid Peroxidation ◽  
Endothelial Cells ◽  
T Lymphocytes ◽  
Palmitic Acid ◽  
Reactive Oxygen ◽  
Insulin Receptors ◽  
Oxygen Species ◽  
Aortic Endothelial Cells ◽  
Human T Lymphocytes

Hyperglycemia and reactive oxygen species mediate apoptosis in aortic endothelial cells through Janus kinase 2

2005 ◽  
Vol 43 (5) ◽  
pp. 320-326 ◽  
Author(s):  
Amany Tawfik ◽  
Liming Jin ◽  
Amy K.L. Banes-Berceli ◽  
Ruth B. Caldwell ◽  
Safia Ogbi ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Reactive Oxygen ◽  
Janus Kinase ◽  
Oxygen Species ◽  
Janus Kinase 2 ◽  
Aortic Endothelial Cells ◽  
Aortic Endothelial

Oxidized phospholipids mediate occludin expression and phosphorylation in vascular endothelial cells

2006 ◽  
Vol 290 (2) ◽  
pp. H674-H683 ◽  
Author(s):  
Lucas DeMaio ◽  
Mahsa Rouhanizadeh ◽  
Srinivasa Reddy ◽  
Alex Sevanian ◽  
Juliana Hwang ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Reactive Oxygen ◽  
Diffusive Flux ◽  
Oxygen Species ◽  
Aortic Endothelial Cells ◽  
Zonula Occludens ◽  
Bovine Aortic Endothelial Cells ◽  
Zonula Occludens 1 ◽  
Aortic Endothelial

Oxidized l-α-1-palmitoyl-2-arachidonoyl- sn-glycero-3-phosphorylcholine (OxPAPC), a component of minimally modified LDL, induces production of proinflammatory cytokines and development of atherosclerotic lesions. We tested the hypothesis that OxPAPC alters expression, phosphorylation, and localization of tight junction (TJ) proteins, particularly occludin, a transmembrane TJ protein. OxPAPC reduced total occludin protein and increased occludin phosphorylation dose dependently (10–50 μg/ml) and time dependently in bovine aortic endothelial cells. OxPAPC decreased occludin mRNA and reduced the immunoreactivity of zonula occludens-1 at the cell-cell contacts. Furthermore, OxPAPC increased the diffusive flux of 10-kDa dextran in a dose-dependent manner. O2−· production by bovine aortic endothelial cells increased nearly twofold after exposure to OxPAPC. Also, enzymatic generation of O2−· by xanthine oxidase-lumazine and H2O2 by glucose oxidase-glucose increased occludin phosphorylation, implicating reactive oxygen species as modulators of the OxPAPC effects on occludin phosphorylation. Superoxide dismutase and/or catalase blocked the effects of OxPAPC on occludin protein content and phosphorylation, occludin mRNA, zonula occludens-1 immunoreactivity, and diffusive flux of 10-kDa dextran. These findings suggest that changes in TJ proteins are potential mechanisms by which OxPAPC compromises the barrier properties of the vascular endothelium. OxPAPC-induced disruption of TJs, which likely facilitates transmigration of LDL and inflammatory cells into the subendothelial layers, may be mediated by reactive oxygen species.

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