scholarly journals Characterization of HCoV-229E fusion core: Implications for structure basis of coronavirus membrane fusion

2006 ◽  
Vol 345 (3) ◽  
pp. 1108-1115 ◽  
Author(s):  
Cheng Liu ◽  
Youjun Feng ◽  
Feng Gao ◽  
Qiangmin Zhang ◽  
Ming Wang
Keyword(s):  
Membrane Fusion

scholarly journals Characterization of a Structural Intermediate of Flavivirus Membrane Fusion

2007 ◽  
Vol 3 (2) ◽  
pp. e20 ◽  
Author(s):  
Karin Stiasny ◽  
Christian Kössl ◽  
Jean Lepault ◽  
Félix A Rey ◽  
Franz X Heinz
Keyword(s):  
Membrane Fusion

scholarly journals Sequential SNARE disassembly and GATE-16–GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion

2002 ◽  
Vol 157 (7) ◽  
pp. 1161-1173 ◽  
Author(s):  
Joyce M.M. Müller ◽  
James Shorter ◽  
Richard Newman ◽  
Katrin Deinhardt ◽  
Yuval Sagiv ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Atp Hydrolysis ◽  
Golgi Membrane ◽  
Complex Assembly ◽  
Evolutionarily Conserved ◽  
Golgi Fragmentation ◽  
Fusion Analysis

Characterization of mammalian NSF (G274E) and Drosophila NSF (comatose) mutants revealed an evolutionarily conserved NSF activity distinct from ATPase-dependent SNARE disassembly that was essential for Golgi membrane fusion. Analysis of mammalian NSF function during cell-free assembly of Golgi cisternae from mitotic Golgi fragments revealed that NSF disassembles Golgi SNAREs during mitotic Golgi fragmentation. A subsequent ATPase-independent NSF activity restricted to the reassembly phase is essential for membrane fusion. NSF/α-SNAP catalyze the binding of GATE-16 to GOS-28, a Golgi v-SNARE, in a manner that requires ATP but not ATP hydrolysis. GATE-16 is essential for NSF-driven Golgi reassembly and precludes GOS-28 from binding to its cognate t-SNARE, syntaxin-5. We suggest that this occurs at the inception of Golgi reassembly to protect the v-SNARE and regulate SNARE function.

scholarly journals Identification and Characterization of Cell Lines with a Defect in a Post-adsorption Stage of Sendai Virus-mediated Membrane Fusion

2000 ◽  
Vol 275 (23) ◽  
pp. 17549-17555 ◽  
Author(s):  
Akiko Eguchi ◽  
Toru Kondoh ◽  
Hirokazu Kosaka ◽  
Takashi Suzuki ◽  
Hiroshi Momota ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Cell Lines ◽  
Sendai Virus ◽  
Identification And Characterization ◽  
Adsorption Stage

scholarly journals Characterization of the functional requirements of West Nile virus membrane fusion

2009 ◽  
Vol 91 (2) ◽  
pp. 389-393 ◽  
Author(s):  
B. Moesker ◽  
I. A. Rodenhuis-Zybert ◽  
T. Meijerhof ◽  
J. Wilschut ◽  
J. M. Smit
Keyword(s):  
West Nile Virus ◽  
Membrane Fusion ◽  
Functional Requirements ◽  
West Nile

scholarly journals Characterization of hepatitis C virus pseudoparticles by cryo-transmission electron microscopy using functionalized magnetic nanobeads

2010 ◽  
Vol 91 (8) ◽  
pp. 1919-1930 ◽  
Author(s):  
Pierre Bonnafous ◽  
Marie Perrault ◽  
Olivier Le Bihan ◽  
Birke Bartosch ◽  
Dimitri Lavillette ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Membrane Fusion ◽  
Magnetic Beads ◽  
Neutral Ph ◽  
Transmission Electron ◽  
Magnetic Nanobeads

Cell entry and membrane fusion of the hepatitis C virus (HCV) depend on its envelope glycoproteins E1 and E2. HCV pseudotyped particles (HCVpps) are relevant and popular models to study the early steps of the HCV life cycle. However, no structural characterization of HCVpp has been available so far. Using cryo-transmission electron microscopy (cryo-TEM), providing structural information at nanometric resolution, the molecular details of HCVpps and their fusion with liposomes were studied. Cryo-TEM revealed HCVpps as regular 100 nm spherical structures containing the dense retroviral nucleocapsid surrounded by a lipid bilayer. E1–E2 glycoproteins were not readily visible on the membrane surface. Pseudoparticles bearing the E1–E2 glycoproteins of Semliki forest virus looked similar, whereas avian influenza A virus (fowl plague virus) haemagglutinin/neuraminidase-pseudotyped particles exhibited surface spikes. To further characterize HCVpp structurally, a novel method was designed based on magnetic beads covered with anti-HCV antibodies to enrich the samples with particles containing E1–E2. This strategy efficiently sorted HCVpps, which were then directly observed by cryo-TEM in the presence or absence of liposomes at low or neutral pH. After acidification, HCVpps looked the same as at neutral pH and closely contacted the liposomes. These are the first visualizations of early HCV membrane fusion events at the nanometer scale. Furthermore, fluorimetry analysis revealed a relative resistance of HCVpps regarding their fusion capacity when exposed to low pH. This study therefore brings several new molecular details to HCVpp characterization and this efficient strategy of virion immunosorting with magnetic nanobeads is direct, efficient and adaptable to extensive characterization of any virus at a nanometric resolution.

scholarly journals Structural and Metal-Binding Characterization of the C-terminal Metallochaperone Domain of the Membrane Fusion Protein SilB from Cupriavidus Metallidurans CH34

2010 ◽  
Vol 98 (3) ◽  
pp. 247a-248a
Author(s):  
Beate Bersch ◽  
Kheiro-Mouna Derfoufi ◽  
Fabien De Angelis ◽  
Elisabeth Ngonlong Ekendé ◽  
Max Mergeay ◽  
...  
Keyword(s):  
Fusion Protein ◽  
Membrane Fusion ◽  
Metal Binding ◽  
Cupriavidus Metallidurans Ch34 ◽  
Membrane Fusion Protein ◽  
Cupriavidus Metallidurans

scholarly journals Delivery of the Radionuclide 131I Using Cationic Fusogenic Liposomes as Nanocarriers

2021 ◽  
Vol 22 (1) ◽  
pp. 457
Author(s):  
Rejhana Kolašinac ◽  
Dirk Bier ◽  
Laura Schmitt ◽  
Andriy Yabluchanskiy ◽  
Bernd Neumaier ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Cellular Uptake ◽  
Cancer Cell Line ◽  
Drug Carriers ◽  
Cargo Delivery ◽  
Human Blood Cells ◽  
Fusion Ability ◽  
Potential Use

Liposomes are highly biocompatible and versatile drug carriers with an increasing number of applications in the field of nuclear medicine and diagnostics. So far, only negatively charged liposomes with intercalated radiometals, e.g., 64Cu, 99mTc, have been reported. However, the process of cellular uptake of liposomes by endocytosis is rather slow. Cellular uptake can be accelerated by recently developed cationic liposomes, which exhibit extraordinarily high membrane fusion ability. The aim of the present study was the development of the formulation and the characterization of such cationic fusogenic liposomes with intercalated radioactive [131I]I− for potential use in therapeutic applications. The epithelial human breast cancer cell line MDA-MB-231 was used as a model for invasive cancer cells and cellular uptake of [131I]I− was monitored in vitro. Delivery efficiencies of cationic and neutral liposomes were compared with uptake of free iodide. The best cargo delivery efficiency (~10%) was achieved using cationic fusogenic liposomes due to their special delivery pathway of membrane fusion. Additionally, human blood cells were also incubated with cationic control liposomes and free [131I]I−. In these cases, iodide delivery efficiencies remained below 3%.

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