scholarly journals Identification and Characterization of Cell Lines with a Defect in a Post-adsorption Stage of Sendai Virus-mediated Membrane Fusion

2000 ◽  
Vol 275 (23) ◽  
pp. 17549-17555 ◽  
Author(s):  
Akiko Eguchi ◽  
Toru Kondoh ◽  
Hirokazu Kosaka ◽  
Takashi Suzuki ◽  
Hiroshi Momota ◽  
...  
Keyword(s):  
Membrane Fusion ◽  
Cell Lines ◽  
Sendai Virus ◽  
Identification And Characterization ◽  
Adsorption Stage

scholarly journals Identification and characterization of a novel paramyxovirus, porcine parainfluenza virus 1, from deceased pigs

2013 ◽  
Vol 94 (10) ◽  
pp. 2184-2190 ◽  
Author(s):  
Susanna K. P. Lau ◽  
Patrick C. Y. Woo ◽  
Ying Wu ◽  
Annette Y. P. Wong ◽  
Beatrice H. L. Wong ◽  
...  
Keyword(s):  
Rectal Swab ◽  
Sendai Virus ◽  
Parainfluenza Virus ◽  
Mrna Editing ◽  
Viral Loads ◽  
Reverse Transcription Pcr ◽  
Phosphoprotein Gene ◽  
Human Parainfluenza Virus ◽  
Identification And Characterization

We describe the discovery and characterization of a novel paramyxovirus, porcine parainfluenza virus 1 (PPIV-1), from swine. The virus was detected in 12 (3.1 %) of 386 nasopharyngeal and two (0.7 %) of 303 rectal swab samples from 386 deceased pigs by reverse transcription-PCR, with viral loads of up to 106 copies ml−1. Complete genome sequencing and phylogenetic analysis showed that PPIV-1 represented a novel paramyxovirus within the genus Respirovirus, being most closely related to human parainfluenza virus 1 (HPIV-1) and Sendai virus (SeV). In contrast to HPIV-1, PPIV-1 possessed a mRNA editing function in the phosphoprotein gene. Moreover, PPIV-1 was unique among respiroviruses in having two G residues instead of three to five G residues following the A6 run at the editing site. Nevertheless, PPIV-1, HPIV-1 and SeV share common genomic features and may belong to a separate group within the genus Respirovirus. The presence of PPIV-1 in mainly respiratory samples suggests a possible association with respiratory disease, similar to HPIV-1 and SeV.

scholarly journals In Vitro Identification and Characterization of CD133pos Cancer Stem-Like Cells in Anaplastic Thyroid Carcinoma Cell Lines

PLoS ONE ◽  
2008 ◽  
Vol 3 (10) ◽  
pp. e3544 ◽  
Author(s):  
Giovanni Zito ◽  
Pierina Richiusa ◽  
Alessandra Bommarito ◽  
Elvira Carissimi ◽  
Leonardo Russo ◽  
...  
Keyword(s):  
Thyroid Carcinoma ◽  
Cell Lines ◽  
Carcinoma Cell ◽  
Anaplastic Thyroid Carcinoma ◽  
Carcinoma Cell Lines ◽  
Identification And Characterization ◽  
Thyroid Carcinoma Cell

scholarly journals Hi-C detects novel structural variants in HL-60 and HL-60/S4 cell lines

2018 ◽  
Author(s):  
Elsie C. Jacobson ◽  
Ralph S. Grand ◽  
Jo K. Perry ◽  
Mark H. Vickers ◽  
Ada L. Olins ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Lines ◽  
Myeloid Leukemia ◽  
Large Scale ◽  
Rna Seq ◽  
Structural Variants ◽  
The Stability ◽  
Different Sources ◽  
Identification And Characterization

AbstractCancer cell lines often have large structural variants (SVs) that evolve over time. There are many reported differences in large scale SVs between HL-60 and HL-60/S4, two cell lines derived from the same acute myeloid leukemia sample. However, the stability and variability of inter- and intra-chromosomal structural variants between different sources of the same cell line is unknown. Here, we used Hi-C and RNA-seq to identify and compare large SVs in HL-60 and HL-60/S4 cell lines. Comparisons with previously published karyotypes identified novel SVs in both cell lines. Hi-C was used to characterize the known expansion centered on the MYC locus. The MYC expansion was integrated into known locations in HL-60/S4, and a novel location (chr4) in HL-60. The HL-60 cell line has more within-line structural variation than the HL-60/S4 derivative cell line. Collectively we demonstrate the usefulness of Hi-C and with RNA-seq data for the identification and characterization of SVs.

Identification and Characterization of an ALK Inhibitor That Attenuates the Proliferation and Survival of Anaplastic Large Cell Lymphoma-Derived Cell Lines and ALK Dependent Cell Lines.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 2485-2485
Author(s):  
Mangeng Cheng ◽  
Weihua Wan ◽  
Mark Albom ◽  
Linda Weinberg ◽  
Thelma Angeles ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Lines ◽  
Small Molecule ◽  
Large Cell ◽  
Leukemia Cell Line ◽  
Alk Inhibitor ◽  
Ic50 Value ◽  
Alk Positive ◽  
Identification And Characterization

Abstract Anaplastic large cell lymphomas (ALCLs) are a unique subgroup of high grade non-Hodgkin lymphomas. Approximately 60% to 70% of ALCLs contain a t (2:5) (p23; q35) chromosomal rearrangement, generating the nucleophosmin-anaplastic lymphoma kinase (NPM-ALK) fusion protein. NPM-ALK encodes a constitutively activated tyrosine kinase that has been demonstrated to be directly involved in the pathogenesis of ALCLs. Currently there is no optimal therapeutic regimen for ALK positive ALCLs. Even with high dose CHOP (cyclophophamide, doxorubicin, vincristine and prednisone)-based chemotherapy, a substantial number of patients with ALK positive ALCLs have very poor outcome, either failing to enter remission or relapsing within a few months from the start of treatment. This prompted a search for small molecule ALK inhibitors as potential therapeutic agents for these patients. In this report, we describe the identification and characterization of a potent small molecule ALK inhibitor, cmpd-1. Cmpd-1 inhibited the ALK kinase activity in an in vitro enzymatic assay with an IC50 value of 4 nM. In NPM-ALK transfected BaF3 cells and ALCL-derived cell lines, cmpd-1 potently inhibited the tyrosine phosphorylation of NPM-ALK with a cellular IC50 value of 10–30 nM. Treatment with cmpd-1 inhibited IL-3 independent proliferation of BaF3 cells expressing constitutively active NPM-ALK (more than 95% inhibition at 100 nM) and the addition of IL-3 rescued the growth of these cells, while cmpd-1 had little effect on the parental BaF3 cells. Inhibition of NPM-ALK activity by cmpd-1 in ALK positive ALCL-derived cell lines, Karpas-299, Sudhl-1, Sup-M2 and Sr-786, resulted in inhibition of cell proliferation (50–90% inhibition at 100–300 nM) and induction of apoptotic cell death, while having marginal effects on the proliferation and survival of K562, an ALK negative leukemia cell line. These data suggest that the inhibition of cell proliferation and induction of apoptosis by cmpd-1 is an ALK-dependent event. Several putative downstream targets of NPM-ALK, including ERK1/2, Stat3 and Akt, were hypo- or unphosphorylated when the ALK positive cells were treated with cmpd-1. With the potent ALK inhibitory activity, cmpd-1 can be used as a molecular and pharmacological tool to study the biological roles of ALK and to dissect ALK-mediated signaling pathways in ALCLs and possibly other tumor types in which ALK is implicated in their pathogenesis.

scholarly journals A novel bioinformatics pipeline for identification and characterization of fusion transcripts in breast cancer and normal cell lines

2011 ◽  
Vol 39 (15) ◽  
pp. e100-e100 ◽  
Author(s):  
Yan W. Asmann ◽  
Asif Hossain ◽  
Brian M. Necela ◽  
Sumit Middha ◽  
Krishna R. Kalari ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cell Lines ◽  
Normal Cell ◽  
Bioinformatics Pipeline ◽  
Fusion Transcripts ◽  
Identification And Characterization

scholarly journals Identification and Characterization of Gamma‐Synuclein as a PCBP1‐Interacting Protein in Various Cancer Cell Lines

2016 ◽  
Vol 30 (S1) ◽  
Author(s):  
Jane Ko ◽  
Amanda Hunkele ◽  
Hamidah Sultan ◽  
Faith Ikalina ◽  
Alexander Liu ◽  
...  
Keyword(s):  
Cell Lines ◽  
Cancer Cell ◽  
Cancer Cell Lines ◽  
Interacting Protein ◽  
Identification And Characterization
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