Plastid DNA polymerases from higher plants, Arabidopsis thaliana

2005 ◽  
Vol 334 (1) ◽  
pp. 43-50 ◽  
Author(s):  
Yoko Mori ◽  
Seisuke Kimura ◽  
Ai Saotome ◽  
Nobuyuki Kasai ◽  
Norihiro Sakaguchi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Higher Plants ◽  
Dna Polymerases ◽  
Plastid Dna

Utilization of a hydrate cellulose film for investigation of Arabidopsis thaliana L. root system growth and development

2020 ◽  
Vol 36 (1) ◽  
pp. 36-43
Author(s):  
I.O. Konovalova ◽  
T.N. Kudelina ◽  
S.O. Smolyanina ◽  
A.I. Lilienberg ◽  
T.N. Bibikova
Keyword(s):  
Arabidopsis Thaliana ◽  
Root System ◽  
Life Support ◽  
Higher Plants ◽  
Plant Root ◽  
Lateral Roots ◽  
Basic Research ◽  
Cellulose Film ◽  
A New Technique ◽  
Basic Research Project

A new technique for Arabidopsis thaliana cultivation has been proposed that combines the use of a phytogel-based nutrient medium and a hydrophilic membrane of hydrate cellulose film, separating the root system of the plant from the medium thickness. Growth rates of both main and lateral roots were faster in the plants cultivated on the surface of hydrate cellulose film than in the plants grown in the phytogel volume. The location of the root system on the surface of the transparent hydrate film simplifies its observation and analysis and facilitates plant transplantation with preservation of the root system configuration. The proposed technique allowed us to first assess the effect of exogenous auxin on the growth of lateral roots at the 5-6 developmental stage. methods to study plant root systems, hydrate cellulose film, A. thaliana, lateral roots, differential root growth rate, auxin The work was financially supported by the Russian Foundation for Basic Research (Project Bel_mol_a 19-54-04015) and the basic topic of the Russian Academy of Sciences - IBMP RAS «Regularities of the Influence of Extreme Environmental Factors on the Processes of Cultivation of Higher Plants and the Development of Japanese Quail Tissues at Different Stages of its Ontogenesis under the Conditions of Regenerative Life Support Systems».

scholarly journals ATP compartmentation in plastids and cytosol ofArabidopsis thalianarevealed by fluorescent protein sensing

2018 ◽  
Vol 115 (45) ◽  
pp. E10778-E10787 ◽  
Author(s):  
Chia Pao Voon ◽  
Xiaoqian Guan ◽  
Yuzhe Sun ◽  
Abira Sahu ◽  
May Ngor Chan ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Fluorescent Protein ◽  
Higher Plants ◽  
Electron Flow ◽  
Cyclic Electron Flow ◽  
Linear Electron ◽  
Protein Sensing ◽  
Atp Production ◽  
Respiratory Inhibitors ◽  
Sensor Protein

Matching ATP:NADPH provision and consumption in the chloroplast is a prerequisite for efficient photosynthesis. In terms of ATP:NADPH ratio, the amount of ATP generated from the linear electron flow does not meet the demand of the Calvin–Benson–Bassham (CBB) cycle. Several different mechanisms to increase ATP availability have evolved, including cyclic electron flow in higher plants and the direct import of mitochondrial-derived ATP in diatoms. By imaging a fluorescent ATP sensor protein expressed in livingArabidopsis thalianaseedlings, we found that MgATP2−concentrations were lower in the stroma of mature chloroplasts than in the cytosol, and exogenous ATP was able to enter chloroplasts isolated from 4- and 5-day-old seedlings, but not chloroplasts isolated from 10- or 20-day-old photosynthetic tissues. This observation is in line with the previous finding that the expression of chloroplast nucleotide transporters (NTTs) inArabidopsismesophyll is limited to very young seedlings. Employing a combination of photosynthetic and respiratory inhibitors with compartment-specific imaging of ATP, we corroborate the dependency of stromal ATP production on mitochondrial dissipation of photosynthetic reductant. Our data suggest that, during illumination, the provision and consumption of ATP:NADPH in chloroplasts can be balanced by exporting excess reductants rather than importing ATP from the cytosol.

Molecular evolution of plastoms of different higher plants - plastid-DNA-polymorphism and cross-hybridization

1986 ◽  
Vol 6 (1) ◽  
pp. 41-42
Author(s):  
F. Baldauf ◽  
W. Tröbner ◽  
K. Steiner ◽  
K. Fritzsche ◽  
M. Metzlaff
Keyword(s):  
Molecular Evolution ◽  
Dna Polymorphism ◽  
Higher Plants ◽  
Plastid Dna ◽  
Cross Hybridization

scholarly journals Genetic fine-structure of the GA-1 locus in the higher plant Arabidopsis thaliana (L.) Heynh

1983 ◽  
Vol 41 (1) ◽  
pp. 57-68 ◽  
Author(s):  
M. Koornneef ◽  
J. Van Eden ◽  
C. J. Hanhart ◽  
A. M. M. De Jongh
Keyword(s):  
Arabidopsis Thaliana ◽  
Fine Structure ◽  
Higher Plants ◽  
Wild Type ◽  
Higher Plant ◽  
Selfed Progeny ◽  
Intragenic Deletion ◽  
Genetic Length ◽  
Genetic Fine Structure ◽  
Half Diallel

SUMMARYNon-germinating gibberellin (GA) responsive mutants are a powerful tool to study genetic fine structure in higher plants. Nine alleles (EMS-and fast neutron-induced) of the ga-1 locus of Arabidopsis thaliana were tested in a complete half-diallel. No wild type ‘recombinants’ were found in the selfed progeny of 9 homoallelic combinations (in total 3 × 105 plants); in the progenies from the 36 selfed hetero allelics the wild type frequency ranged from zero to 6·6 × 10−4. These frequencies allowed the construction of an internally consistent map for five different sites representing eight alleles. The ninth allele covered three sites and thus behaved like an intragenic deletion. The estimate of the total genetic length of the ga-1 locus was 0·07 cM. The order of the sites was also clearly reflected by the association with proximal outside markers. On the assumption that wild type gametes predominantly arise from reciprocal events, it was shown that a cross-over within the ga-1 locus leads to positive interference in the adjacent region.The results are discussed with respect to the mutagen used, the frequencies found in other plant and Drosophila genes, and the possible occurrence of gene conversion.

scholarly journals Evolutionary History and Activity of RNase H1-Like Proteins in Arabidopsis thaliana

2020 ◽  
Vol 61 (6) ◽  
pp. 1107-1119
Author(s):  
Jan Kuciński ◽  
Sebastian Chamera ◽  
Aleksandra Kmera ◽  
M Jordan Rowley ◽  
Sho Fujii ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Embryonic Development ◽  
Evolutionary History ◽  
Common Ancestor ◽  
Normal Growth ◽  
Plastid Dna ◽  
Growth Conditions ◽  
Replicative Stress ◽  
The Common ◽  
Rna:Dna Hybrids

Abstract RNase H1 is an endonuclease specific toward the RNA strand of RNA:DNA hybrids. Members of this protein family are present in most living organisms and are essential for removing RNA that base pairs with DNA. It prevents detrimental effects of RNA:DNA hybrids and is involved in several biological processes. Arabidopsis thaliana has been previously shown to contain three genes encoding RNase H1 proteins that localize to three distinct cellular compartments. We show that these genes originate from two gene duplication events. One occurred in the common ancestor of dicots and produced nuclear and organellar RNase H1 paralogs. Second duplication occurred in the common ancestor of Brassicaceae and produced mitochondrial- and plastid-localized proteins. These proteins have the canonical RNase H1 activity, which requires at least four ribonucleotides for endonucleolytic digestion. Analysis of mutants in the RNase H1 genes revealed that the nuclear RNH1A and mitochondrial RNH1B are dispensable for development under normal growth conditions. However, the presence of at least one organellar RNase H1 (RNH1B or RNH1C) is required for embryonic development. The plastid-localized RNH1C affects plastid DNA copy number and sensitivity to replicative stress. Our results present the evolutionary history of RNH1 proteins in A. thaliana, demonstrate their canonical RNase H1 activity and indicate their role in early embryonic development.

scholarly journals Nuclear Dynamics in Arabidopsis thaliana

2000 ◽  
Vol 11 (8) ◽  
pp. 2733-2741 ◽  
Author(s):  
Eva Chytilova ◽  
Jiri Macas ◽  
Elwira Sliwinska ◽  
Susanne M. Rafelski ◽  
Georgina M. Lambert ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Laser Scanning ◽  
Fluorescent Protein ◽  
Higher Plants ◽  
Chimeric Protein ◽  
Time Lapse ◽  
Confocal Laser ◽  
Long Distance ◽  
General Applicability ◽  
Living Plant

The nucleus is a definitive feature of eukaryotic cells, comprising twin bilamellar membranes, the inner and outer nuclear membranes, which separate the nucleoplasmic and cytoplasmic compartments. Nuclear pores, complex macromolecular assemblies that connect the two membranes, mediate communication between these compartments. To explore the morphology, topology, and dynamics of nuclei within living plant cells, we have developed a novel method of confocal laser scanning fluorescence microscopy under time-lapse conditions. This is used for the examination of the transgenic expression in Arabidopsis thaliana of a chimeric protein, comprising the GFP (Green-Fluorescent Protein of Aequorea victoria) translationally fused to an effective nuclear localization signal (NLS) and to β-glucuronidase (GUS) from E. coli. This large protein is targeted to the nucleus and accumulates exclusively within the nucleoplasm.  This article provides online access to movies that illustrate the remarkable and unusual properties displayed by the nuclei, including polymorphic shape changes and rapid, long-distance, intracellular movement. Movement is mediated by actin but not by tubulin; it therefore appears distinct from mechanisms of nuclear positioning and migration that have been reported for eukaryotes. The GFP-based assay is simple and of general applicability. It will be interesting to establish whether the novel type of dynamic behavior reported here, for higher plants, is observed in other eukaryotic organisms.

scholarly journals Arabidopsis thaliana organelles mimic the T7 phage DNA replisome with specific interactions between Twinkle protein and DNA polymerases Pol1A and Pol1B

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Stewart A. Morley ◽  
Antolín Peralta-Castro ◽  
Luis G. Brieba ◽  
Justin Miller ◽  
Kai Li Ong ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Dna Polymerases ◽  
Specific Interactions ◽  
Phage Dna ◽  
T7 Phage

Aspterric Acid and 6-Hydroxymellein, Inhibitors of Pollen Development in Arabidopsis thaliana, Produced by Aspergillus terreus

2002 ◽  
Vol 57 (5-6) ◽  
pp. 459-464 ◽  
Author(s):  
Atsumi Shimada ◽  
Miyako Kusano ◽  
Sumiyo Takeuchi ◽  
Shozo Fujioka ◽  
Tomohisa Inokuchi ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Microscopic Examination ◽  
Pollen Development ◽  
Aspergillus Terreus ◽  
Higher Plants ◽  
Molecular Investigation ◽  
Microspore Stage ◽  
Stage 1

Aspterric acid (1) and 6-hydroxymellein (2), inhibitors of pollen development in Arabidopsis thaliana, have been isolated fromthe fungus Aspergillus terreus. 1 and 2 inhibited the pollen development at concentrations of 38 and 52 μᴍ, respectively. The microscopic examination of pollen development suggested that the inhibition by the treatment with 1 caused at meiosis and the inhibition by the treatment with 2 caused at microspore stage. 1 and 2 could be useful agents for the molecular investigation of anther and pollen development in higher plants.

Sign in / Sign up

Export Citation Format

Share Document