Effects of chronic testosterone administration on the degree of preference for a high-fat diet and body weight in gonadal-intact and ovariectomized female rats

2018 ◽  
Vol 349 ◽  
pp. 102-108 ◽  
Author(s):  
Takeshi Iwasa ◽  
Toshiya Matsuzaki ◽  
Kiyohito Yano ◽  
Mayila Yiliyasi ◽  
Akira Kuwahara ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Female Rats ◽  
High Fat ◽  
Testosterone Administration

scholarly journals SUN-239 Effects of High-Fat Diet-Induced Obesity on Pulsatile LH Secretion and Hypothalamic KISS1 Expression in Female Rats

2020 ◽  
Vol 4 (Supplement_1) ◽  
Author(s):  
Shiori Minabe ◽  
Kinuyo Iwata ◽  
Youki Watanabe ◽  
Hitoshi Ozawa
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Pulse Frequency ◽  
Female Rats ◽  
Standard Diet ◽  
High Fat ◽  
Estrous Cycles ◽  
Menstrual Dysfunction ◽  
Estrous Cyclicity ◽  
Lh Secretion

Abstract Female obesity is associated with menstrual dysfunction leading to anovulation and infertility. It has recently been reported obesity-induced infertility is involved in the dysfunction of a kisspeptin neuron, a key player in reproduction via direct stimulation of gonadotropin releasing hormone (GnRH) and subsequent gonadotropin release in mammalian species. Previous studies reported that obesity due to high-fat diet (HFD) for 8 months induced a disruption in estrous cyclicity, caused by a decrease in Kiss1 (coding kisspeptin) expression in the hypothalamic arcuate nucleus (ARC) in female rodents. Here we showed the effects of shorter-term (4 months) HFD on pulsatile LH secretion and hypothalamic Kiss1 expression to show pathogenic mechanism underlying obesity-induced infertility. Female Wistar-Imamichi strain rats (7 weeks old) fed on either a standard diet (10% calories from fat) or a high-fat diet (45% calories from fat) for 4 months. Estrous cyclicity and body weight were monitored regularly. All animals were implanted with a jugular catheter and collected blood samples to analyze pulsatile LH secretion, after a week of the ovariectomy with low-dose replacement estradiol to negate influence of changes in ovarian steroid levels and mimic diestrous levels of plasma estrogen. On the next day of the blood sampling, rats were perfused with 0.05 M PBS followed by 4% paraformaldehyde and their brains were collected for in situ hybridization of Kiss1 and Gnrh1. The HFD-fed rats showed progressive increases in body weight, along with hyperphagia and adipose tissue accumulation, compared with control animals. Fifty-eight percent of the HFD-fed rats exhibited irregular estrous cycles, whereas remaining HFD-fed rats showed regular cycles. Two out of 7 rats showing HFD-induced irregular estrous cycles exhibited profound suppression of the LH pulse frequency and the number of Kiss1-expressing cells in the ARC, whereas remaining HFD-fed rats showed normal LH pulses and ARC Kiss1 expressions. The number of Kiss1-expressing cells in the ARC had close positive correlation with LH pulse frequency (R2=0.6872, P<0.001) in both groups. Additionally, the number of Kiss1- or Gnrh1-expressing cells in the anteroventral periventricular nucleus or the preoptic area, were comparable between groups. Taken together, our finding reveals the possibility that irregular menstruation was also induced by changes in the kisspeptin-GnRH independent pathway during the incipient stage of obese infertility.

The effects of ovariectomy and lifelong high-fat diet consumption on body weight, appetite, and lifespan in female rats

2018 ◽  
Vol 97 ◽  
pp. 25-30 ◽  
Author(s):  
Takeshi Iwasa ◽  
Toshiya Matsuzaki ◽  
Kiyohito Yano ◽  
Minoru Irahara
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Female Rats ◽  
High Fat

scholarly journals Effect of Citrus Limon Essential Oil on Lipid Profile and Obesity in Wistar Rats

2021 ◽  
pp. 1-5
Author(s):  
Souhaila Benchaoui ◽  
Meriem Gueracheha ◽  
Asma Boutebsou
Keyword(s):  
Body Weight ◽  
Essential Oil ◽  
Lipid Profile ◽  
High Fat Diet ◽  
Intraperitoneal Administration ◽  
Female Rats ◽  
Abdominal Circumference ◽  
Citrus Limon ◽  
Standard Diet ◽  
High Fat

Obesity causes many pathologies; no therapeutic axis has provided an effective solution to this problem. The use of herbal medicine with healthy food and physical activity is recommended, and essential oils are the best known of these natural substances. The objective of this study is to evaluate the effect of Citrus limon essential oil on body weight and lipid profile. The plant material is the lemon; the extraction method is hydrodistillation. The experiment lasted 20 days and involved 15 female rats (2 to 3 months old) weighing between 160g and 230g. The rats were divided into 3 groups: the first receiving a standard diet, the second receiving a high-fat diet, and the third receiving a high-fat diet and treated with essential oil. The extraction yield is 1.69%. The physicochemical characteristics comply with AFNOR. This study shows that a high-lipid diet induces obesity characterized by hyperlipidemia. Intraperitoneal administration of the essential oil caused a decrease in body weight, abdominal circumference, plasma total cholesterol, and triglycerides and an increase in HDL-cholesterol. Considering this study, we found that lemon essential oil has beneficial effects on metabolic alterations. Its inclusion in the diet may help improve the metabolic profile and reduce the incidence of obesity and its long-term complications.

scholarly journals Sex Differences in Gut Microbiome in High-Fat-Diet Fed Rats

2020 ◽  
Author(s):  
Ying Shi ◽  
Fangzhi Yue ◽  
Lin Xing ◽  
Shanyu Wu ◽  
Lin Wei ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Body Weight ◽  
Sex Differences ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Metabolic Diseases ◽  
Normal Diet ◽  
Female Rats ◽  
Male Rats ◽  
High Fat

Abstract Background: Sex differences in obesity and related metabolic diseases are well recognized, however, the mechanism has not been elucidated. Gut microbiota and its metabolites may play a vital role in the development of obesity and metabolic diseases. The aim of the present study was to investigate sex differences in gut microbiota and its metabolites in a high-fat-diet (HFD) obesity rats and identify microbiota genera potentially contributing to such differences in obesity and non-alcoholic fatty liver disease (NAFLD) susceptibility.Methods: Sprague–Dawley rats were divided into four groups (eight animals per group): (1) male rats on a normal diet (MND), (2) male rats on HFD (MHFD), (3) female rats on a normal diet (FND), and (4) female rats on HFD (FHFD). Body weight, liver pathology, gut microbiota and short/medium chain fatty acids in colon contents were compared between different sexes.Results: HFD induced more body weight gain and fat storage in female rats, however, lower hepatic steatosis in FHFD than in MHFD rats was observed. When considering gut microbiota composition, FHFD rats had lower microbiome diversity than MHFD. A significant increase of Firmicutes phylum, along with Bilophila and Blautia genus was detected in MHFD rats, as compared with FHFD, which showed increased relative abundance of Murimonas. Moreover, propionic and lauric acid levels were higher in FHFD than those in MHFD rats. Conclusions: HFD induced sex-related alterations in gut microbiome and fatty acids. Furthermore, the genus Bilophila, Blautia and Murimonas might contribute to sex differences observed in obesity and NAFLD susceptibility.

scholarly journals Effect of Ajwa date seed powder on visceral fat depots and inflammatory response in high fat fed Sprague Dawley female rats.

2021 ◽  
Vol 10 (4) ◽  
pp. 200-205
Author(s):  
Farhana Yasmin Bhatti ◽  
Fariha Ahmad Khan ◽  
Abdul Mudabbir Rehan ◽  
Zoobia Irum ◽  
Sadia Chiragh
Keyword(s):  
Body Weight ◽  
Visceral Fat ◽  
High Fat Diet ◽  
Female Rats ◽  
P Value ◽  
Sprague Dawley ◽  
High Fat ◽  
Fat Depots ◽  
Date Seed ◽  
Perinephric Fat

ABSTRACT Background: Central obesity a worldwide metabolic and cosmetic problem poses significant health risk. Ajwa date seed has antioxidant property and its high fiber content may prevent fat absorption and reduce fat deposition. Objective: To evaluate the effect of Ajwa date seed powder on visceral fat depots and inflammatory response in high fat fed Sprague Dawley female rats. Methodology: Thirty-six healthy rats of four weeks age were divided into three groups and followed for 12 weeks. Group A (normal control) received regular diet. Group B (HFD control) received high fat diet, while group C (HFD+Ajwa group) received high fat diet along with 2% Ajwa date seed powder. Body weight was measured weekly. Blood sample was drawn for the estimation of serum IL-6 and leptin levels by ELISA method at 12th week. After which rats were euthanized; perinephric fat was removed and weighed in grams. Results: HFD+Ajwa group gained less body weight as compared to HFD control group (p value = 0.012). There was a remarkable reduction in perinephric fat weight (p value ≤ 0.001) and level of IL-6 in HFD+Ajwa group (p value ≤ 0.001) as compared to HFD control. There was no significant difference in serum leptin level of the rats of all groups (p value = 0.567). Conclusion: Ajwa date seed can prevent visceral adiposity and gain in body weight. Moreover, it has anti-inflammatory effect; but no significant effect on satiety hormone. Key Words: Obesity, Visceral fat, P. dactylifera L (Ajwa date) seed, IL-6, Leptin.

High fat diet-induced estrus cycle disruption: effects of Ficus asperifolia

2015 ◽  
Vol 12 (3) ◽  
Author(s):  
Esther Ngadjui ◽  
Pepin Alango Nkeng-Efouet ◽  
Telesphore Benoit Nguelefack ◽  
Albert Kamanyi ◽  
Pierre Watcho
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Body Weight Gain ◽  
Tween 80 ◽  
The Body ◽  
Female Rats ◽  
Vaginal Smear ◽  
High Fat ◽  
Methanolic Extracts ◽  
Obese Rats

Abstract:: Obesity was reached by feeding female rats with a HFD for 10 weeks. Vaginal smear was observed daily for 3 weeks after animals were obese. Then, 70 animals with abnormal estrus cyclicity were selected and partitioned into two sets of 35 animals. Each set was further divided into seven groups of five rats. These obese rats with disrupted estrus cyclicity were orally administered the aqueous and methanolic extracts (100 and 500 mg/kg), distilled water (10 mL/kg), 5% Tween 80 (10 mL/kg) or lutenyl (0.8 µg/kg) once a day for 1 week (set I) or 4 weeks (set II). Estrus cyclicity, body weight gain, hematocrit, lipid profile, ovarian, uterine and hepatic growth indices were determined at the end of each treatment.: HFD increased the body weight of the animals by 27% and disrupted the estrus cyclicity by 98.44%. Aqueous extract (100 mg/kg) of: Our data support the anecdotal claims of

scholarly journals Characterizing the Retinal Phenotype in the High-Fat Diet and Western Diet Mouse Models of Prediabetes

Cells ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 464 ◽  
Author(s):  
Bright Asare-Bediako ◽  
Sunil Noothi ◽  
Sergio Li Calzi ◽  
Baskaran Athmanathan ◽  
Cristiano Vieira ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Fundus Photography ◽  
Vascular Density ◽  
Sensitivity Index ◽  
High Fat ◽  
Permeability Studies ◽  
Induced Changes ◽  
Acellular Capillaries

We sought to delineate the retinal features associated with the high-fat diet (HFD) mouse, a widely used model of obesity. C57BL/6 mice were fed either a high-fat (60% fat; HFD) or low-fat (10% fat; LFD) diet for up to 12 months. The effect of HFD on body weight and insulin resistance were measured. The retina was assessed by electroretinogram (ERG), fundus photography, permeability studies, and trypsin digests for enumeration of acellular capillaries. The HFD cohort experienced hypercholesterolemia when compared to the LFD cohort, but not hyperglycemia. HFD mice developed a higher body weight (60.33 g vs. 30.17g, p < 0.0001) as well as a reduced insulin sensitivity index (9.418 vs. 62.01, p = 0.0002) compared to LFD controls. At 6 months, retinal functional testing demonstrated a reduction in a-wave and b-wave amplitudes. At 12 months, mice on HFD showed evidence of increased retinal nerve infarcts and vascular leakage, reduced vascular density, but no increase in number of acellular capillaries compared to LFD mice. In conclusion, the HFD mouse is a useful model for examining the effect of prediabetes and hypercholesterolemia on the retina. The HFD-induced changes appear to occur slower than those observed in type 2 diabetes (T2D) models but are consistent with other retinopathy models, showing neural damage prior to vascular changes.

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