scholarly journals Characterizing the Retinal Phenotype in the High-Fat Diet and Western Diet Mouse Models of Prediabetes

Cells ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 464 ◽  
Author(s):  
Bright Asare-Bediako ◽  
Sunil Noothi ◽  
Sergio Li Calzi ◽  
Baskaran Athmanathan ◽  
Cristiano Vieira ◽  
...  
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Fundus Photography ◽  
Vascular Density ◽  
Sensitivity Index ◽  
High Fat ◽  
Permeability Studies ◽  
Induced Changes ◽  
Acellular Capillaries

We sought to delineate the retinal features associated with the high-fat diet (HFD) mouse, a widely used model of obesity. C57BL/6 mice were fed either a high-fat (60% fat; HFD) or low-fat (10% fat; LFD) diet for up to 12 months. The effect of HFD on body weight and insulin resistance were measured. The retina was assessed by electroretinogram (ERG), fundus photography, permeability studies, and trypsin digests for enumeration of acellular capillaries. The HFD cohort experienced hypercholesterolemia when compared to the LFD cohort, but not hyperglycemia. HFD mice developed a higher body weight (60.33 g vs. 30.17g, p < 0.0001) as well as a reduced insulin sensitivity index (9.418 vs. 62.01, p = 0.0002) compared to LFD controls. At 6 months, retinal functional testing demonstrated a reduction in a-wave and b-wave amplitudes. At 12 months, mice on HFD showed evidence of increased retinal nerve infarcts and vascular leakage, reduced vascular density, but no increase in number of acellular capillaries compared to LFD mice. In conclusion, the HFD mouse is a useful model for examining the effect of prediabetes and hypercholesterolemia on the retina. The HFD-induced changes appear to occur slower than those observed in type 2 diabetes (T2D) models but are consistent with other retinopathy models, showing neural damage prior to vascular changes.

scholarly journals Evaluation of voglibose on body weight in rats

2019 ◽  
Vol 8 (6) ◽  
pp. 1159
Author(s):  
Sarita Mulkalwar ◽  
Tanya Gupta ◽  
Vishwanath Kulkarni ◽  
A. V. Tilak ◽  
B. T. Rane ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Type 2 Diabetes ◽  
Type 2 Diabetes Mellitus ◽  
Body Weight ◽  
High Fat Diet ◽  
Normal Diet ◽  
High Fat ◽  
Diet Induced Obesity ◽  
Obesity Drugs

Background: As of 2018, 2.1 billion people nearly 30% of the world’s population are either obese or overweight. Worldwide obesity has nearly tripled since 1975. It is an emerging health problem with major adverse effects on health. It is a risk factor for many chronic diseases but is best known for its role in metabolic syndrome, which can lead to type 2 diabetes mellitus as well as cardiovascular diseases. Anti-obesity drugs are available but have many side effects. Voglibose, an antidiabetic drug, is an alpha glucosidase inhibitor which shows promising results in the reduction of body weight with minimal side effects.Methods: Voglibose (7 mg/kg) was administered to rats fed with normal laboratory chows and high fat diet to see its effect on body weight, body mass index, abdominal and thoracic circumference, and lipid profile at the end of 12 weeks.Results: Administration of voglibose significantly reduced food consumption, feed efficiency and increase in body weight induced by high fat diet in rats. Rats fed on normal diet also showed reductions in the same parameters, suggesting its weight lowering effect. Reductions in the anthropometric measurements, hypolipidemic effects and glucose lowering effects were also observed.Conclusions: Voglibose prevented high fat diet-induced obesity and improvement in metabolic profile, which ultimately has systemic effects on body weight in rats. Further studies are needed to see its potential therapeutic use in obese patients with type 2 diabetes mellitus, and related complications.

scholarly journals Polyphenol-Rich Fraction of Brown AlgaEcklonia cavaCollected from Gijang, Korea, Reduces Obesity and Glucose Levels in High-Fat Diet-Induced Obese Mice

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Eun Young Park ◽  
Eung Hwi Kim ◽  
Mi Hwi Kim ◽  
Young Wan Seo ◽  
Jung Im Lee ◽  
...  
Keyword(s):  
Type 2 Diabetes ◽  
Body Weight ◽  
Glucose Tolerance ◽  
Mrna Expression ◽  
Inflammatory Cytokines ◽  
High Fat Diet ◽  
Obese Mice ◽  
High Fat ◽  
Glucose Levels

Ecklonia cava (E. cava)is a brown alga that has beneficial effects in models of type 1 and type 2 diabetes. However, the effects ofE. cavaextracts on diet-induced obesity and type 2 diabetes have not been specifically examined. We investigated the effects ofE. cavaon body weight, fat content, and hyperglycemia in high-fat diet- (HFD) induced obese mice and sought the mechanisms involved. C57BL/6 male mice were fed a HFD (60% fat) diet or normal chow. After 3 weeks, the HFD diet group was given extracts (200 mg/kg) ofE. cavaharvested from Jeju (CA) or Gijang (G-CA), Korea or PBS by oral intubation for 8 weeks. Body weights were measured weekly. Blood glucose and glucose tolerance were measured at 7 weeks, and fat pad content and mRNA expression of adipogenic genes and inflammatory cytokines were measured after 8 weeks of treatment. G-CA was effective in reducing body weight gain, body fat, and hyperglycemia and improving glucose tolerance as compared with PBS-HFD mice. The mRNA expression of adipogenic genes was increased, and mRNA expression of inflammatory cytokines and macrophage marker gene was decreased in G-CA-treated obese mice. We suggest that G-CA reduces obesity and glucose levels by anti-inflammatory actions and improvement of lipid metabolism.

scholarly journals Hypoglycemic Activity through a Novel Combination of Fruiting Body and Mycelia ofCordyceps militarisin High-Fat Diet-Induced Type 2 Diabetes Mellitus Mice

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
Sung-Hsun Yu ◽  
Szu-Yu Tina Chen ◽  
Wei-Shan Li ◽  
Navneet Kumar Dubey ◽  
Wei-Hong Chen ◽  
...  
Keyword(s):  
Diabetes Mellitus ◽  
Body Weight ◽  
Adipose Tissue ◽  
Blood Glucose ◽  
Fruiting Body ◽  
High Fat Diet ◽  
Oral Glucose ◽  
High Fat ◽  
Type 2 Dm

Diabetes mellitus (DM) is currently ranked among leading causes of death worldwide in which type 2 DM is reaching an epidemic proportion. Hypoglycemic medications for type 2 DM have either proven inadequate or posed adverse effects; therefore, the Chinese herbal products are under investigation as an alternative treatment. In this study, a novel combination of fruiting body and mycelia powder of herbalCordyceps militarisnumber 1 (CmNo1) was administered to evaluate their potential hypoglycemic effects in high-fat diet- (HFD-) induced type 2 DM in C57BL/6J mice. Body weight, fasting blood glucose (FBG), oral glucose tolerance test (OGTT), and blood biochemistry indexes were measured. Results indicated that CmNo1 lowered the blood glucose level by increasing insulin sensitivity, while no change in body weight was observed. Increased protein expression of IRS-1, pIRS-1, AKT, pAKT, and GLUT-4 in skeletal muscle and adipose tissue was found indicating restoration of insulin signaling. Additionally, PPAR-γexpression in adipose tissue restored the triglyceride and cholesterol levels. Finally, our results suggest that CmNo1 possesses strong hypoglycemic, anticholesterolemic, and antihypertriglyceridemic actions and is more economical alternate for DM treatment.

scholarly journals Comparative evaluation of the effect of Ocimum sanctum and metformin on serum lipid profile in high fat diet fed diabetic rats

2019 ◽  
Vol 8 (3) ◽  
pp. 589
Author(s):  
Shailendra Mishra ◽  
Quazi Shahir Ahmed ◽  
Kauser Sayedda
Keyword(s):  
Body Weight ◽  
High Fat Diet ◽  
Serum Lipid ◽  
Ethanolic Extract ◽  
Diabetic Rats ◽  
Ocimum Sanctum ◽  
Control Group ◽  
Lipid Levels ◽  
High Fat

Background: Dyslipidaemia is an important risk factor for development of macrovascular complications in type 2 diabetes mellitus. Ocimum sanctum (OS) and metformin have shown to have antihyperlipidaemic effects. The present study was undertaken  to evaluate the effects of  OS and Metformin on body weight & plasma lipid  levels of high fat diet fed diabetic ratsMethods: Total of 30 male wistar  rats (100-150gm) were obtained. Animals were fed with a high fat diet throughout the study (6 weeks). Diabetes was induced by using single intra-peritoneal injection of Streptozotocin 50mg/kg at the end of 4 weeks.  Diabetic rats were divided into groups of 6 each and treated as follows: Group 1- Diabetic control, was given vehicle orally. Group 2- O.S. ethanolic extract 100mg/kg body weight orally for 14 days. Group 3- O.S. ethanolic extract  200mg/kg body weight orally for 14 days. Group 4-  Metformin 100mg/day for 14 daysResults: At the end of 4 weeks, body weight of rats were significantly increased (p <0.05). Maximum weight gain was seen in control group whereas weight gain was least in O.S. 200mg/kg group (p >0.05). Decrease in body weight was seen in metformin group. Abdominal circumference of rats also showed similar pattern (p >0.05).  OS 200 caused significant reduction in serum LDL levels (p <0.05) and significant rise of serum HDL levels (p <0.05) as compared to control group. Metformin also favourably affected the lipid profile and its effects were not significantly different from effects of OS 200 (p> 0.05).Conclusions: Present study revealed that Ocimum Sanctum caused significant reduction in serum lipid levels in high fat diet fed diabetic rats. Metformin  also exhibited antihyperlipidaemic activity. So, it is concluded that OS or metformin alone or in combination  could be a novel adjunct to diet and life style modification for the management of dyslipidaemia in type 2 diabetes.  Further studies are required to confirm the antidyslipidaemic activities of individual phytoconstituents of Ocimum sanctum.

scholarly journals Effect of endurance exercise on oxidative stress marker malondialdehyde in type 2 diabetic mice.

2020 ◽  
Vol 27 (07) ◽  
pp. 1493-1498
Author(s):  
Sana Akram ◽  
Maimona Tabssum ◽  
Maryam Rao ◽  
Hamid Javaid Qureshi
Keyword(s):  
Oxidative Stress ◽  
Body Weight ◽  
Endurance Exercise ◽  
High Fat Diet ◽  
Exercise Program ◽  
Oxidative Stress Marker ◽  
Diabetic Mice ◽  
High Fat ◽  
Type 2 Diabetic

Diabetes is associated with oxidative stress and has a significant role in the pathophysiology of the disease and its complications. Exercise training is a powerful therapeutic approach in diabetes and has protective effects against the progress of its complications. Objectives: The aim of this study was to investigate the effect of an endurance exercise program on the oxidative stress marker malondialdehyde (MDA) in high fat diet-low dose streptozotocin induced type 2 diabetic mouse model. Study Design: Randomized Control trial. Setting: Department of Physiology, Akhtar Saeed Medical and Dental College, Lahore. Period: August 2017 to August 2018. Material & Methods: 60 male albino mice were fed a high fat diet containing 60% kCal as fat for 4 weeks. This was followed by intra peritoneal injection of 40mg/kg body weight streptozotocin, given on three consecutive days. Mice with fasting blood glucose more than 250mg/dl after a week were considered diabetic. Half the mice underwent an exercise program which comprised of a 20 minute swimming session per day, with a 6% body weight load attached to the tail of mice, 3 days a week, for 4 weeks. The level of MDA was estimated in both groups using TBARs method. Results: Mean malondialdehyde level was significantly (p˂0.05) reduced in diabetic mice that underwent endurance exercise training. Conclusion: This study highlights the important role of endurance exercise in reducing oxidative stress in diabetes.

scholarly journals High-fat diet negatively impacts both metabolic and behavioral health in outbred heterogeneous stock rats

2020 ◽  
Vol 52 (9) ◽  
pp. 379-390
Author(s):  
Aaron W. Deal ◽  
Osborne Seshie ◽  
Anne Lenzo ◽  
Nicholas Cooper ◽  
Noelle Ozimek ◽  
...  
Keyword(s):  
Body Weight ◽  
Glucose Tolerance ◽  
Behavioral Health ◽  
High Fat Diet ◽  
Forced Swim Test ◽  
Fat Pad ◽  
High Fat ◽  
Negative Effects ◽  
Heterogeneous Stock ◽  
Induced Changes

Obesity is influenced by genetics and diet and has wide ranging comorbidities, including anxiety and depressive disorders. Outbred heterogeneous stock (HS) rats are used for fine-genetic mapping of complex traits and may be useful for understanding gene by diet interactions. In this study, HS rats were fed diets containing 60% kcal from fat (high-fat diet, HFD) or 10% kcal from fat (low-fat diet, LFD) and tested for metabolic ( study 1) and behavioral ( study 2) outcomes. In study 1, we measured glucose tolerance, fasting glucose and insulin, fat pad weights and despair-like behavior in the forced swim test (FST). In study 2, we assessed anxiety-like (elevated plus maze, EPM; open field test, OFT) and despair-like/coping (splash test, SpT; and FST) behaviors. Body weight and food intake were measured weekly in both studies. We found negative effects of HFD on metabolic outcomes, including increased body weight and fat pad weights, decreased glucose tolerance, and increased fasting insulin. We also found negative effects of HFD on despair-like/coping and anxiety-like behaviors. These include increased immobility in the FST, decreased open arm time in the EPM, and increased movement and rest episodes and decreased rearing in the OFT. The diet-induced changes in EPM and OFT were independent of overall locomotion. Additionally, diet-induced changes in OFT behaviors were independent of adiposity, while adiposity was a confounding factor for EPM and FST behavior. This work establishes the HS as a model to study gene by diet interactions affecting metabolic and behavioral health.

scholarly journals Gut microbiota impairs insulin clearance during obesity

2020 ◽  
Author(s):  
Kevin P. Foley ◽  
Soumaya Zlitni ◽  
Brittany M. Duggan ◽  
Nicole G. Barra ◽  
Fernando F. Anhê ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
High Fat Diet ◽  
Insulin Clearance ◽  
Small Community ◽  
High Fat ◽  
Short Term ◽  
Germ Free ◽  
Impaired Insulin ◽  
Induced Changes

AbstractHyperinsulinemia can be a cause and consequence of obesity and insulin resistance. Increased insulin secretion and reduced insulin clearance can contribute to hyperinsulinemia. The triggers for changes in insulin clearance during obesity are ill-defined. We found that oral antibiotics mitigated impaired insulin clearance in mice fed a high fat diet (HFD) for 12 weeks or longer. Short-term HFD feeding and aging did not alter insulin clearance in mice. Germ-free mice colonized with microbes from HFD-fed mice had impaired insulin clearance, but not C-peptide clearance, and only after mice were colonized for 6 weeks and then HFD-fed. Five bacterial taxa predicted >90% of the variance in insulin clearance. Our data indicate that gut microbes are an independent and transmissible factor that regulates obesity-induced changes in insulin clearance. A small cluster of microbes may be a target for mitigating defects in insulin clearance and the progression of obesity and Type 2 Diabetes. We propose that a small community in the gut microbiota can impair insulin clearance and increase insulin load and the risk of complications from hyperinsulinemia.

scholarly journals Suppression of High-Fat Diet–Induced Obesity by Platycodon Grandiflorus in Mice Is Linked to Changes in the Gut Microbiota

2020 ◽  
Vol 150 (9) ◽  
pp. 2364-2374
Author(s):  
Weixin Ke ◽  
Germán Bonilla-Rosso ◽  
Philipp Engel ◽  
Pan Wang ◽  
Fang Chen ◽  
...  
Keyword(s):  
Body Weight ◽  
Gut Microbiota ◽  
High Fat Diet ◽  
Control Diet ◽  
Intestinal Barrier ◽  
Amplicon Sequencing ◽  
Disease Suppression ◽  
Intestinal Barrier Function ◽  
High Fat ◽  
Induced Changes

ABSTRACT Background The root of Platycodon grandiflorus (PG) has a long-standing tradition in the Asian diet and herbal medicine, because of its anti-inflammatory and antiobesity effects. Changes in the gut microbiota can have dietary effects on host health, which suggests a relation between the 2. Objectives The aim of our study was to investigate the relation between PG-mediated suppression of obesity and the composition and functioning of the gut microbiota. Methods Six-week-old male C57BL/6J mice were fed either a control diet (CON, 10% kcal from fat), a high-fat diet (HFD, 60% kcal from fat), or a PG-supplemented HFD for 18 wk. PG was administered by oral gavage at 2 g · kg body weight−1 · d−1. Body weight and food intake were monitored. Lipid metabolism, inflammation, and intestinal barrier function were determined. Amplicon sequencing of the bacterial 16S ribosomal RNA gene was used to explore gut microbiota structure, and nontargeted metabolomics analysis was performed to investigate metabolite concentrations in fecal samples. Results We found that PG significantly ameliorated HFD-induced inflammation, recovered intestinal barrier integrity (reduced permeability by 39% , P = 0.008), reduced fat accumulation by 26% (P = 0.009), and changed the expression of key genes involved in the development of white adipose tissue (P &lt; 0.05) in HFD-fed mice to similar levels in CON mice. Moreover, PG attenuated HFD-induced changes in the gut microbiota; it especially increased Allobaculum (7.3-fold, P = 0.002) relative to HFD, whereas CON was 15.2-fold of HFD (P = 0.002). These changes by PG were associated with an increase in the production of SCFAs (butyrate and propionate, P &lt; 0.001) and other carbohydrate-related metabolites known to have a major role in disease suppression. Conclusions Our study demonstrated that PG beneficially changed the gut microbiota and the gut metabolome in HFD-fed mice, and suggests that the antiobesity effects of PG may be mediated via changes in gut microbiota composition and metabolic activity.

Abstract P198: Increased Urinary Angiotensinogen Reflects Intrarenal Renin-angiotensin System Activation in High Fat Diet Induced-type 2diabetic Mice

Hypertension ◽  
2016 ◽  
Vol 68 (suppl_1) ◽  
Author(s):  
Virginia Reverte ◽  
Diego Castro Musial ◽  
Michael R Gallaty ◽  
Carla B Rosales ◽  
Alberto J Parra-Vitela ◽  
...  
Keyword(s):  
Body Weight ◽  
Renal Injury ◽  
High Fat Diet ◽  
Renin Angiotensin System ◽  
High Fat ◽  
Angiotensin System ◽  
Renin Angiotensin ◽  
Type 2 Dm ◽  
Intrarenal Ras

Obesity is a risk factor for diabetes mellitus (DM) and patients with DM are often hypertensive and exhibit inappropriate activation of the intrarenal renin-angiotensin system (RAS), which increases the risk for diabetic nephropathy and progression to chronic kidney disease. Enhanced intrarenal AGT levels contribute to increased intrarenal Angiotensin II levels. To examine the role of an activated RAS in DM, we measured urinary AGT along with albuminuria and other metabolic parameters in high fat diet induced-type 2 DM mice. Immediately after weaning, male C57BL/6J mice (N=18) were fed either a normal fat diet (18 % Kcal from fat; CT mice) or high fat diet (42.0 % Kcal from fat; HF mice) for 24 wk. Body weight (BW), food intake, and systolic blood pressure (SBP) by telemetry, were measured weekly. Glucose tolerance test (GTT) and homeostasis model assessment index of insulin resistance (HOMA-IR) were further assayed. At wks 0, 12, and 24 on dietary regimen, measurements of albuminuria and uAGT by ELISA were used as markers of renal injury and intrarenal RAS activation, respectively. Body weight increased by 140% in HF mice compared to 66 % in CT mice (from 18±0.5 to 44±1.4 g and 17±0.6 to 28±0.6 g, respectively) with no differences in daily energy intake (15.02±1.3 kcal/day and 15.4±0.8 kcal/day). Plasma glucose, area under curve (AUC) of GTT, and HOMA-IR were significantly increased in HF mice (121± 6mg/dL; 32,865±6,222; 5±1) compared to CT mice (220± 27mg/dL; 55,549±4,611; 54±9). In HF mice, SBP was augmented by wk 10 compared to CT mice (126±5 vs. 113±1 mmHg; P<0.05). Interestingly, no differences in albuminuria were found between HF and CT mice either at wk 12(50±13 vs. 40±6 ug/day) or at wk 24 (57±7 vs. 43±3 ug/day). However, uAGT excretion was increased by wk 24 in HF mice but not in CT mice ( wk 0 : 4±1.5 vs. 4±1.5ng/day; wk 12 : 4.6±2.3 vs. 2.3±0.9 ng/day; wk 24 : 11.8±2.9 vs. 3.9±0.3 ng/day; P<0.05). During HF diet induced-type 2 DM, the elevation of uAGT reflects the increase of intrarenal RAS which may contribute to the development of renal injury.

scholarly journals Medium-Chain Triglycerides Lower Blood Lipids and Body Weight in Streptozotocin-Induced Type 2 Diabetes Rats

Nutrients ◽  
2018 ◽  
Vol 10 (8) ◽  
pp. 963 ◽  
Author(s):  
Ming-Hua Sung ◽  
Fang-Hsuean Liao ◽  
Yi-Wen Chien
Keyword(s):  
Type 2 Diabetes ◽  
Body Weight ◽  
Lipid Metabolism ◽  
Soybean Oil ◽  
High Fat Diet ◽  
Density Lipoprotein ◽  
High Fat ◽  
Medium Chain Triglycerides ◽  
Low Fat

Medium-chain triglycerides (MCTs) are distinguished from other triglycerides in that each fat molecule consists of 6 to 12 carbons in length. MCTs and long-chain triglycerides (LCTs) are absorbed and utilized in different ways. The aim of this study was to assess the effects of replacing soybean oil with MCT oil, in a low- or high-fat diet, on lipid metabolism in rats with streptozotocin-induced type 2 diabetes mellitus (T2DM). There were, thirty-two T2DM Sprague-Dawley rats divided into low-fat-soybean oil (LS), low-fat-MCT oil (LM), high-fat-soybean oil (HS), and high-fat-MCT oil (HM) groups. After 8 weeks, blood sugar, serum lipids, liver lipids, and enzyme activities related to lipid metabolism were measured. Under a high-fat diet condition, replacement of soybean oil with MCT oil lowered serum low-density lipoprotein cholesterol (LDL-C), non-esterified fatty acids, and liver total cholesterol; whilst it increased serum high-density lipoprotein cholesterol (HDL-C) and the HDL-C/LDL-C ratio. A low-fat diet with MCT oil resulted in lower body weight and reproductive white adipose tissues compared to the HS groups, and higher hepatic acyl-CoA oxidase activities (the key enzyme in the peroxisomal beta-oxidation) compared to the LS group in T2DM rats. In conclusion, MCTs showed more protective effects on cardiovascular health in T2DM rats fed a high-fat diet, by improving serum lipid profiles and reducing hepatic total cholesterol.

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