scholarly journals Recipient T-Cell Repertoire Diversity after Double Umbilical Cord Transplantation Correlates with Non-Relapse Mortality and Survival

2014 ◽  
Vol 20 (2) ◽  
pp. S243-S244 ◽  
Author(s):  
Sarah Nikiforow ◽  
Haesook T. Kim ◽  
Sean M. McDonough ◽  
Ryan Emerson ◽  
David Hamm ◽  
...  
Keyword(s):  
T Cell ◽  
Umbilical Cord ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity

scholarly journals Characterization of human cytomegalovirus peptide–specific CD8+ T-cell repertoire diversity following in vitro restimulation by antigen-pulsed dendritic cells

Blood ◽  
2002 ◽  
Vol 99 (1) ◽  
pp. 213-223 ◽  
Author(s):  
Karl Peggs ◽  
Stephanie Verfuerth ◽  
Arnold Pizzey ◽  
Jenni Ainsworth ◽  
Paul Moss ◽  
...  
Keyword(s):  
T Cells ◽  
Dendritic Cells ◽  
T Cell ◽  
Viral Replication ◽  
Cd8 T Cells ◽  
Cd8 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity ◽  
Clonal Composition

Under conditions of impaired T-cell immunity, human cytomegalovirus (HCMV) can reactivate from lifelong latency, resulting in potentially fatal disease. A crucial role for CD8+ T cells has been demonstrated in control of viral replication, and high levels of HCMV-specific cytotoxic T-lymphocytes are seen in immunocompetent HCMV-seropositive individuals despite very low viral loads. Elucidation of the minimum portion of the anti-HCMV T-cell repertoire that is required to suppress viral replication requires further study of clonal composition. The ability of dendritic cells to take up and process exogenous viral antigen by constitutive macropinocytosis was used to study HCMV-specific T-cell memory in the absence of viral replication. The specificity and clonal composition of the CD8+ T-cell responses were evaluated using HLA tetrameric complexes and T-cell receptor β chain (TCRBV) spectratypic analyses. There was a skewed reactivity toward the matrix protein pp65, with up to 40-fold expansion of CD8+ T cells directed toward a single peptide-MHC combination. Individual expansions detected on TCRBV spectratype analysis were HCMV-specific and composed of single or highly restricted numbers of clones. There was preferential TCRBV gene usage (BV6.1/6.2, BV8, and BV13 in HLA-A*0201+ individuals) but lack of conservation of CDR3 length and junctional motifs between donors. While there was a spectrum of TCR repertoire diversity directed toward individual MHC-peptide combinations between donors, a relatively small number of clones appeared to predominate the response in each case. These data provide further insight into the range of anti-HCMV responses and will aid the design and monitoring of adoptive immunotherapy protocols.

scholarly journals Cytomegalovirus Exposure in the Elderly Does Not Reduce CD8 T Cell Repertoire Diversity

2018 ◽  
Vol 202 (2) ◽  
pp. 476-483 ◽  
Author(s):  
Paul Lindau ◽  
Rithun Mukherjee ◽  
Miriam V. Gutschow ◽  
Marissa Vignali ◽  
Edus H. Warren ◽  
...  
Keyword(s):  
T Cell ◽  
The Elderly ◽  
Cd8 T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity

scholarly journals Altered T-cell repertoire diversity in septic shock patients

Critical Care ◽  
2014 ◽  
Vol 18 (Suppl 1) ◽  
pp. P223
Author(s):  
N Takeyama ◽  
A Tomino ◽  
M Hashiba ◽  
A Hirakawa ◽  
T Hattori ◽  
...  
Keyword(s):  
Septic Shock ◽  
T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity

scholarly journals The Umbilical Cord Blood αβ T-Cell Repertoire: Characteristics of a Polyclonal and Naive but Completely Formed Repertoire

Blood ◽  
1998 ◽  
Vol 91 (1) ◽  
pp. 340-346 ◽  
Author(s):  
Laurent Garderet ◽  
Nicolas Dulphy ◽  
Corinne Douay ◽  
Nathalie Chalumeau ◽  
Véronique Schaeffer ◽  
...  
Keyword(s):  
T Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Allogeneic Transplantation ◽  
Cell Receptor ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Promising Alternative ◽  
Β Chain

Abstract Umbilical cord blood (CB) constitutes a promising alternative to bone marrow for allogeneic transplantation and is increasingly used because of the reduced severity of graft-versus-host disease after CB transplantation. We have compared the T-cell receptor β chain (TCRB) diversity of CB lymphocytes with that of adult lymphocytes by analyzing the complementarity determining region 3 (CDR3) size heterogeneity. In marked contrast to adult samples, we observed bell-shaped profiles in all of the 22 functional β-chain variable (BV) subfamilies that reflect the lack of prior antigenic stimulation in CB samples. However, the mean CDR3 size and BV usage were comparable between CB and adult samples. BJ2 (65%) segments were used preferentially to BJ1 (35%), especially BJ2S7, BJ2S5, BJ2S3, and BJ2S1, in both CB and in adult lymphocytes. We therefore conclude that although naive as reflected by the heterogeneity of the CDR3 size, the TCRBV repertoire appears fully constituted at birth. The ability to expand TCRB subfamilies was confirmed by stimulation with staphylococcal superantigens toxic shock syndrome toxin-1 and staphylococcal enterotoxin A. This study provides the basis for future analysis of the T-cell repertoire reconstitution following umbilical CB transplantation.

scholarly journals Corrigendum: Germline-Encoded TCR-MHC Contacts Promote TCR V Gene Bias in Umbilical Cord Blood T Cell Repertoire

2020 ◽  
Vol 11 ◽  
Author(s):  
Kai Gao ◽  
Lingyan Chen ◽  
Yuanwei Zhang ◽  
Yi Zhao ◽  
Ziyun Wan ◽  
...  
Keyword(s):  
T Cell ◽  
Cord Blood ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
V Gene

T-cell repertoire diversity and clonal expansions in normal and clinical samples

1995 ◽  
Vol 16 (4) ◽  
pp. 176-181 ◽  
Author(s):  
Christophe Pannetier ◽  
Jos Even ◽  
Philippe Kourilsky
Keyword(s):  
T Cell ◽  
Clinical Samples ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity

scholarly journals Dacarbazine Treatment before Peptide Vaccination Enlarges T-Cell Repertoire Diversity of Melan-A–Specific, Tumor-Reactive CTL in Melanoma Patients

2010 ◽  
Vol 70 (18) ◽  
pp. 7084-7092 ◽  
Author(s):  
Belinda Palermo ◽  
Duilia Del Bello ◽  
Alessandra Sottini ◽  
Federico Serana ◽  
Claudia Ghidini ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Repertoire ◽  
Peptide Vaccination ◽  
Cell Repertoire ◽  
Specific Tumor ◽  
Repertoire Diversity ◽  
Melanoma Patients

scholarly journals Impact of T Cell Repertoire Diversity on Mortality Following Cord Blood Transplantation

2020 ◽  
Vol 10 ◽  
Author(s):  
F. Milano ◽  
R. O. Emerson ◽  
R. Salit ◽  
K. A. Guthrie ◽  
L. A. Thur ◽  
...  
Keyword(s):  
T Cell ◽  
Cord Blood ◽  
Cord Blood Transplantation ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Blood Transplantation ◽  
Repertoire Diversity

scholarly journals T Cell Repertoire Diversity Is Decreased in Type 1 Diabetes Patients

2016 ◽  
Vol 14 (6) ◽  
pp. 338-348 ◽  
Author(s):  
Yin Tong ◽  
Zhoufang Li ◽  
Hua Zhang ◽  
Ligang Xia ◽  
Meng Zhang ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity ◽  
Diabetes Patients

Prognostic effects of peripheral and tumor-infiltrating T-cell repertoire diversity in ovarian cancer.

2016 ◽  
Vol 34 (15_suppl) ◽  
pp. 5546-5546
Author(s):  
Kunle Odunsi ◽  
Takemasa Tsuji ◽  
Kevin H. Eng ◽  
James Brian Szender ◽  
Junko Matsuzaki ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
T Cell ◽  
T Cell Repertoire ◽  
Cell Repertoire ◽  
Repertoire Diversity
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