scholarly journals 42: Insulin-like growth factor I positively regulates thymic function by expansion of thymocyte precursors and thymic epithelial cells

2007 ◽  
Vol 13 (2) ◽  
pp. 18 ◽  
Author(s):  
Y.-W. Chu ◽  
S. Schmitz ◽  
B. Choudhury ◽  
R.E. Gress
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Thymic Epithelial Cells ◽  
Insulin Like Growth Factor ◽  
Thymic Function ◽  
Factor I ◽  
Growth Factor I

Transdifferentiation of NRP-152 rat prostatic basal epithelial cells toward a luminal phenotype: regulation by glucocorticoid, insulin-like growth factor-I and transforming growth factor-beta

1999 ◽  
Vol 112 (2) ◽  
pp. 169-179 ◽  
Author(s):  
D. Danielpour
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Tight Junction ◽  
Cell Line ◽  
Transforming Growth Factor ◽  
Insulin Like Growth Factor ◽  
Tgf Beta ◽  
Factor I ◽  
Growth Factor I ◽  
Basal Epithelial Cells

The role of basal epithelial cells in prostatic function, development and carcinogenesis is unknown. The ability of basal prostatic epithelial cells to acquire a luminal phenotype was explored in vitro using the NRP-152 rat dorsal-lateral prostate epithelial cell line as a model system. NRP-152, which was spontaneously immortalized and clonally derived, is an androgen-responsive and nontumorigenic cell line that has a basal cell phenotype under normal growth conditions. However, when placed in mitogen-deficient media, these cells undergo a dramatic morphological change to a luminal phenotype. Under these growth-restrictive conditions, immunocytochemical analysis shows that NRP-152 cells acquire the luminal markers Z0-1 (a tight-junction associated protein), occludin (integral tight-junction protein), and cytokeratin 18, and lose the basal markers cytokeratins 5 and 14. Total protein and mRNA levels of cytokeratins 8, 18, c-CAM 105 (the calcium-independent cell adhesion molecule) and Z0-1, as detected by western and/or northern blot analyses, respectively, are induced, while cytokeratin 5 and 15 are lost, and occludin is unchanged. Concomitant with this differentiation, expression of transforming growth factor-beta2 (TGF-beta2), TGF-beta3, and TGF-beta receptor type II (TbetaRII) is induced, while those of TGF-beta1 and TbetaRI remain essentially unchanged. Mitogens, such as insulin-like growth factor-I and dexamethasone inhibit luminal differentiation, while exogenous TGF-beta induces such differentiation. These data together with TGF-beta neutralization experiments using pan-specific antibody implicate an important role for autocrine TGF-beta in the induction of the luminal differentiation.

Protein kinases C-γ and -δ are involved in insulin-like growth factor I–induced migration of colonic epithelial cells

1999 ◽  
Vol 116 (1) ◽  
pp. 64-77 ◽  
Author(s):  
Frédéric André ◽  
Véronique Rigot ◽  
Maryse Remacle-Bonnet ◽  
José Luis ◽  
Gilbert Pommier ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Protein Kinases ◽  
Insulin Like Growth Factor ◽  
Colonic Epithelial Cells ◽  
Factor I ◽  
Growth Factor I
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