scholarly journals Detection and viral load monitoring of BK virus in hemorrhagic cystitis complicating bone marrow transplant patients

2004 ◽  
Vol 10 ◽  
pp. 87-88
Author(s):  
P. Manna ◽  
D. Wall ◽  
M. Grimely ◽  
S. Arnoldi ◽  
A. Vats
Keyword(s):  
Bone Marrow ◽  
Viral Load ◽  
Bone Marrow Transplant ◽  
Hemorrhagic Cystitis ◽  
Marrow Transplant ◽  
Bk Virus ◽  
Transplant Patients ◽  
Viral Load Monitoring ◽  
Load Monitoring

scholarly journals Quantitative Real-Time PCR with Automated Sample Preparation for Diagnosis and Monitoring of Cytomegalovirus Infection in Bone Marrow Transplant Patients

2004 ◽  
Vol 50 (5) ◽  
pp. 846-856 ◽  
Author(s):  
Kyeong Man Hong ◽  
Hazim Najjar ◽  
Mary Hawley ◽  
Richard D Press
Keyword(s):  
Bone Marrow ◽  
Viral Load ◽  
Antiviral Therapy ◽  
Real Time ◽  
Bone Marrow Transplant ◽  
Real Time Pcr ◽  
Marrow Transplant ◽  
Diagnostic Sensitivity ◽  
Pcr Assay ◽  
Transplant Patients

Abstract Background: In bone marrow and stem cell transplant patients, the widespread use of preemptive cytomegalovirus (CMV) antiviral therapy necessitates faster, more precise, and more sensitive quantitative laboratory methods for serial viral load monitoring. Methods: We developed a novel CMV viral load assay using real-time PCR of plasma DNA prepared by an automated robotic workstation. Fluorescent hybridization probes directed at the glycoprotein B (gB) gene (or EcoRI D region) of CMV were used to detect and quantify PCR products. The β-globin gene was amplified in parallel to control for the efficiency of the extraction and PCR steps. Results: The assay was linear (R = 0.999) from a lower detection limit of 125 copies/mL to 5 × 109 copies/mL with a PCR efficiency of 1.975 (gB) or 2.02 (EcoRI D). The viral loads determined by PCRs directed at these two different viral targets were no different (n = 53; R = 0.928). The interassay CV was 3.5%, and the intraassay CV was 1–4%. Compared with a commercially available quantitative competitive PCR assay (Roche MONITOR; R = 0.59), the mean CMV viral load by real-time PCR was 3.1 times higher (mean ratio; P = 0.002). The diagnostic sensitivity and specificity of the real-time assay were 96% and 100%, respectively (n = 147), compared with 74% and 98% for a qualitative PCR assay (Roche AMPLICOR). On a subset of samples, the diagnostic sensitivity of viral culture was no greater than 50% (n = 44). Of 1115 clinical referral samples from 252 patients, 10% of the samples and 18% of the patients had low-level CMV viremia (median, 500 copies/mL). In this predominantly (85%) bone marrow transplant testing cohort, serial CMV viral load results were the predominant clinical trigger for the initiation, monitoring, and cessation of preemptive antiviral therapy. Conclusions: The combination of automated DNA preparation and semiautomated real-time fluorescent PCR detection allows for a sensitive, precise, and accurate high-throughput assay of CMV viral load that can be used as the laboratory trigger for preemptive antiviral therapy.

Total Hip Arthroplasty in Very Young Bone Marrow Transplant Patients

2015 ◽  
Vol 24 (02) ◽  
Author(s):  
Cameron K. Ledford ◽  
Alexander R. Vap ◽  
Michael P. Bolognesi ◽  
Samuel S. Wellman
Keyword(s):  
Bone Marrow ◽  
Total Hip Arthroplasty ◽  
Bone Marrow Transplant ◽  
Hip Arthroplasty ◽  
Marrow Transplant ◽  
Total Hip ◽  
Transplant Patients

Monitoring Cardiotoxicity in Bone Marrow Transplant Patients With Prior Anthracycline (AC) Therapy

2021 ◽  
Vol 30 ◽  
pp. S198-S199
Author(s):  
P. Emerson ◽  
S. Mahendran ◽  
L. Stefani ◽  
T. Deshmukh ◽  
S. Trivedi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplant ◽  
Marrow Transplant ◽  
Transplant Patients

scholarly journals Detection of Human Polyomaviruses in Urine from Bone Marrow Transplant Patients: Comparison of Electron Microscopy with PCR

2004 ◽  
Vol 50 (2) ◽  
pp. 306-312 ◽  
Author(s):  
Stefan S Biel ◽  
Andreas Nitsche ◽  
Andreas Kurth ◽  
Wolfgang Siegert ◽  
Muhsin Özel ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Real Time ◽  
Bone Marrow Transplant ◽  
Real Time Pcr ◽  
Marrow Transplant ◽  
Test System ◽  
Urine Samples ◽  
Clinical Samples ◽  
Transplant Patients

Abstract Background: We studied electron microscopy (EM) as an appropriate test system for the detection of polyomavirus in urine samples from bone marrow transplant patients. Methods: We evaluated direct EM, ultracentrifugation (UC) before EM, and solid-phase immuno-EM (SPIEM). The diagnostic accuracy of EM was studied by comparison with a real-time PCR assay on 531 clinical samples. Results: The detection rate of EM was increased by UC and SPIEM. On 531 clinical urine samples, the diagnostic sensitivity of EM was 47% (70 of 149) with a specificity of 100%. We observed a linear relationship between viral genome concentration and the proportion of urine samples positive by EM, with a 50% probability for a positive EM result for urine samples with a polyomavirus concentration of 106 genome-equivalents (GE)/mL; the probability of a positive EM result was 0% for urine samples with <103 GE/mL and 100% for urine samples containing 109 GE/mL. Conclusions: UC/EM is rapid and highly specific for polyomavirus in urine. Unlike real-time PCR, EM has low sensitivity and cannot quantify the viral load.

scholarly journals Infectious Complications in Bone Marrow Transplant Patients

BMJ ◽  
1971 ◽  
Vol 1 (5739) ◽  
pp. 18-23 ◽  
Author(s):  
C. O. Solberg ◽  
H. J. Meuwissen ◽  
R. N. Needham ◽  
R. A. Good ◽  
J. M. Matsen
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplant ◽  
Marrow Transplant ◽  
Infectious Complications ◽  
Transplant Patients

Efficacy of Amlodipine in Pediatric Bone Marrow Transplant Patients

1998 ◽  
Vol 37 (1) ◽  
pp. 31-35 ◽  
Author(s):  
Sohail Khattak ◽  
John W. Rogan ◽  
E. Fred Saunders ◽  
Jochen G. W. Theis ◽  
Gerald S. Arbus ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplant ◽  
Marrow Transplant ◽  
Transplant Patients
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