Comparative proteomic analysis of thiol proteins in the liver after oxidative stress induced by diethylnitrosamine

2013 ◽  
Vol 1834 (12) ◽  
pp. 2528-2538 ◽  
Author(s):  
Diana I. Aparicio-Bautista ◽  
Julio I. Pérez-Carreón ◽  
Nora Gutiérrez-Nájera ◽  
Juan P. Reyes-Grajeda ◽  
Jaime Arellanes-Robledo ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Thiol Proteins

scholarly journals Proteome Profiling of PMJ2-R and Primary Peritoneal Macrophages

2021 ◽  
Vol 22 (12) ◽  
pp. 6323
Author(s):  
Alexander L. Rusanov ◽  
Peter M. Kozhin ◽  
Olga V. Tikhonova ◽  
Victor G. Zgoda ◽  
Dmitry S. Loginov ◽  
...  
Keyword(s):  
Proteomic Analysis ◽  
Living Organism ◽  
Peritoneal Macrophages ◽  
In Vitro Models ◽  
Individual Characteristics ◽  
Essential Proteins ◽  
Comparative Proteomic Analysis ◽  
Immortalized Cell ◽  
The Individual

In vitro models are often used for studying macrophage functions, including the process of phagocytosis. The application of primary macrophages has limitations associated with the individual characteristics of animals, which can lead to insufficient standardization and higher variability of the obtained results. Immortalized cell lines do not have these disadvantages, but their responses to various signals can differ from those of the living organism. In the present study, a comparative proteomic analysis of immortalized PMJ2-R cell line and primary peritoneal macrophages isolated from C57BL/6 mice was performed. A total of 4005 proteins were identified, of which 797 were quantified. Obtained results indicate significant differences in the abundances of many proteins, including essential proteins associated with the process of phagocytosis, such as Elmo1, Gsn, Hspa8, Itgb1, Ncf2, Rac2, Rack1, Sirpa, Sod1, C3, and Msr1. These findings indicate that outcomes of studies utilizing PMJ2-R cells as a model of peritoneal macrophages should be carefully validated. All MS data are deposited in ProteomeXchange with the identifier PXD022133.

scholarly journals Comparative proteomic analysis of mitochondria isolated from Euglena gracilis under aerobic and hypoxic conditions

PLoS ONE ◽  
2019 ◽  
Vol 14 (12) ◽  
pp. e0227226 ◽  
Author(s):  
Shun Tamaki ◽  
Kohei Nishino ◽  
Takahisa Ogawa ◽  
Takanori Maruta ◽  
Yoshihiro Sawa ◽  
...  
Keyword(s):  
Euglena Gracilis ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Hypoxic Conditions

Comparative proteomic analysis of whole saliva from chronic periodontitis patients

2010 ◽  
Vol 73 (7) ◽  
pp. 1334-1341 ◽  
Author(s):  
Lorena Da Rós Gonçalves ◽  
Márcia Regina Soares ◽  
Fábio C.S. Nogueira ◽  
Carlos Garcia ◽  
Danielle Resende Camisasca ◽  
...  
Keyword(s):  
Chronic Periodontitis ◽  
Proteomic Analysis ◽  
Comparative Proteomic Analysis ◽  
Whole Saliva
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