NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis

2006 ◽  
Vol 1760 (11) ◽  
pp. 1723-1731 ◽  
Author(s):  
Alberta Tomassini Miccheli ◽  
Alfredo Miccheli ◽  
Roberta Di Clemente ◽  
Mariacristina Valerio ◽  
Pierpaolo Coluccia ◽  
...  
Keyword(s):  
Cell Growth ◽  
Metabolic Profiling ◽  
Culture Media ◽  
Hepatoma Cells ◽  
Media Analysis ◽  
Human Hepatoma ◽  
Human Hepatoma Cells

Inhibitory Effects of Lycopene on the Adhesion, Invasion, and Migration of SK-Hep1 Human Hepatoma Cells

2006 ◽  
Vol 231 (3) ◽  
pp. 322-327 ◽  
Author(s):  
Eun-Sun Hwang ◽  
Hyong Joo Lee
Keyword(s):  
Cell Growth ◽  
Hepatoma Cell ◽  
Hepatoma Cells ◽  
Hepatoma Cell Line ◽  
Dependent Manner ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Human Hepatoma Cell ◽  
Invasion And Migration ◽  
And Migration

Lycopene, which is the predominant carotenoid in tomatoes and tomato-based foods, may protect humans against various cancers. Effects of lycopene on the adhesion, invasion, migration, and growth of the SK-Hep1 human hepatoma cell line were investigated. Lycopene inhibited cell growth in dose-dependent manners, with growth inhibition rates of 5% and 40% at 0.1 μM and 50 μM lycopene, respectively, after 24 hrs of incubation. Similarly, after 48 hrs of incubation, lycopene at 5 μM and 10 μM decreased the cell numbers by 30% and 40%, respectively. Lycopene decreased the gelatinolytic activities of both matrix metalloproteinase (MMP)-2 and MMP-9, which were secreted from the SK-Hep1 cells. Incubation of SK-Hep1 cells with 110 μM of lycopene for 60 mins significantly inhibited cell adhesion to the Matrigel-coated substrate in a concentration-dependent manner. To study invasion, SK-Hep1 cells were grown either on Matrigel-coated Transwell membranes or in 24-well plates. The cells were treated sequentially for 24 hrs with lycopene before the start of the invasion assays. Cell growth and death were assessed under the same conditions. The invasion of SK-Hep1 cells treated with lycopene was significantly reduced to 28.3% and 61.9% of the control levels at 5 μM and 10 μM lycopene, respectively (P < 0.05). In the migration assay, lycopene-treated cells showed lower levels of migration than untreated cells. These results demonstrate the antimetastatic properties of lycopene in inhibiting the adhesion, invasion, and migration of SK-Hep1 human hepatoma cells.

Sirolimus inhibits growth of human hepatoma cells in contrast to tacrolimus which promotes cell growth

2002 ◽  
Vol 34 (5) ◽  
pp. 1392-1393 ◽  
Author(s):  
G Schumacher ◽  
M Oidtmann ◽  
S Rosewicz ◽  
J.M Langrehr ◽  
S Jonas ◽  
...  
Keyword(s):  
Cell Growth ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells

scholarly journals TGF-β1-induced cell growth arrest and partial differentiation is related to the suppression of Id1 in human hepatoma cells

2006 ◽  
Author(s):  
Bazarragchaa Damdinsuren ◽  
Hiroaki Nagano ◽  
Motoi Kondo ◽  
Javzandulam Natsag ◽  
Hiroyuki Hanada ◽  
...  
Keyword(s):  
Cell Growth ◽  
Growth Arrest ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Cell Growth Arrest ◽  
Partial Differentiation ◽  
Tgf Β1

scholarly journals Induction of differentiation and metabolic reprogramming in human hepatoma cells by adult human serum

2017 ◽  
Author(s):  
Rineke Steenbergen ◽  
Martin Oti ◽  
Rob ter Horst ◽  
Wilson Tat ◽  
Chris Neufeldt ◽  
...  
Keyword(s):  
Human Serum ◽  
Metabolic Profile ◽  
Cultured Cells ◽  
Culture Media ◽  
Hepatoma Cells ◽  
Metabolic Reprogramming ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Organ Specific

AbstractTissue culture medium routinely contains fetal bovine serum (FBS). Here we show that culturing human hepatoma cells in their native, adult serum (human serum, HS) results in the restoration of key morphological and metabolic features of normal liver cells. When moved to HS, these cells show differential transcription of 22-32% of the genes, stop proliferating, and assume a hepatocyte-like morphology. Metabolic analysis shows that the Warburg-like metabolic profile, typical for FBS-cultured cells, is replaced by a diverse metabolic profile consistent within vivohepatocytes. We demonstrate the formation of large lipid and glycogen stores, increased glycogenesis, increased β-oxidation, increased ketogenesis, and decreased glycolysis. Finally, organ-specific functions are restored, including xenobiotics degradation and secretion of bile, very low density lipoprotein, and albumin. Thus, organ-specific functions are not necessarily lost in cell cultures, but might be merely suppressed in FBS. Together, we showed that cells that are representative of normal physiology can be produced from cancer cells simply by replacing FBS by HS in culture media. The effect of serum is often overseen in cell culture and we provide a detailed study in the changes that occur, provide insight in some of the serum components that may play a role in the establishment of the different phenotypes, and discuss how these finding might be beneficial to a variety of research fields.

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