The effect of lycopene on cell growth and oxidative DNA damage of Hep3B human hepatoma cells

BioFactors ◽  
2005 ◽  
Vol 23 (3) ◽  
pp. 129-139 ◽  
Author(s):  
Yun Ok Park ◽  
Eun-Sun Hwang ◽  
Tae Wha Moon
Keyword(s):  
Dna Damage ◽  
Cell Growth ◽  
Oxidative Dna Damage ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells

Inhibitory Effects of Lycopene on the Adhesion, Invasion, and Migration of SK-Hep1 Human Hepatoma Cells

2006 ◽  
Vol 231 (3) ◽  
pp. 322-327 ◽  
Author(s):  
Eun-Sun Hwang ◽  
Hyong Joo Lee
Keyword(s):  
Cell Growth ◽  
Hepatoma Cell ◽  
Hepatoma Cells ◽  
Hepatoma Cell Line ◽  
Dependent Manner ◽  
Human Hepatoma ◽  
Human Hepatoma Cells ◽  
Human Hepatoma Cell ◽  
Invasion And Migration ◽  
And Migration

Lycopene, which is the predominant carotenoid in tomatoes and tomato-based foods, may protect humans against various cancers. Effects of lycopene on the adhesion, invasion, migration, and growth of the SK-Hep1 human hepatoma cell line were investigated. Lycopene inhibited cell growth in dose-dependent manners, with growth inhibition rates of 5% and 40% at 0.1 μM and 50 μM lycopene, respectively, after 24 hrs of incubation. Similarly, after 48 hrs of incubation, lycopene at 5 μM and 10 μM decreased the cell numbers by 30% and 40%, respectively. Lycopene decreased the gelatinolytic activities of both matrix metalloproteinase (MMP)-2 and MMP-9, which were secreted from the SK-Hep1 cells. Incubation of SK-Hep1 cells with 110 μM of lycopene for 60 mins significantly inhibited cell adhesion to the Matrigel-coated substrate in a concentration-dependent manner. To study invasion, SK-Hep1 cells were grown either on Matrigel-coated Transwell membranes or in 24-well plates. The cells were treated sequentially for 24 hrs with lycopene before the start of the invasion assays. Cell growth and death were assessed under the same conditions. The invasion of SK-Hep1 cells treated with lycopene was significantly reduced to 28.3% and 61.9% of the control levels at 5 μM and 10 μM lycopene, respectively (P < 0.05). In the migration assay, lycopene-treated cells showed lower levels of migration than untreated cells. These results demonstrate the antimetastatic properties of lycopene in inhibiting the adhesion, invasion, and migration of SK-Hep1 human hepatoma cells.

NMR-based metabolic profiling of human hepatoma cells in relation to cell growth by culture media analysis

2006 ◽  
Vol 1760 (11) ◽  
pp. 1723-1731 ◽  
Author(s):  
Alberta Tomassini Miccheli ◽  
Alfredo Miccheli ◽  
Roberta Di Clemente ◽  
Mariacristina Valerio ◽  
Pierpaolo Coluccia ◽  
...  
Keyword(s):  
Cell Growth ◽  
Metabolic Profiling ◽  
Culture Media ◽  
Hepatoma Cells ◽  
Media Analysis ◽  
Human Hepatoma ◽  
Human Hepatoma Cells

Sirolimus inhibits growth of human hepatoma cells in contrast to tacrolimus which promotes cell growth

2002 ◽  
Vol 34 (5) ◽  
pp. 1392-1393 ◽  
Author(s):  
G Schumacher ◽  
M Oidtmann ◽  
S Rosewicz ◽  
J.M Langrehr ◽  
S Jonas ◽  
...  
Keyword(s):  
Cell Growth ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells

Protective effect of amifostine on busulfan induced DNA damage in human hepatoma cells

2016 ◽  
Vol 27 (1) ◽  
pp. 52-57 ◽  
Author(s):  
Nasrin Ghassemi-Barghi ◽  
Mahmoud Etebari ◽  
Abbas Jafarian-Dehkordi
Keyword(s):  
Dna Damage ◽  
Protective Effect ◽  
Hepatoma Cells ◽  
Human Hepatoma ◽  
Human Hepatoma Cells
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