Maintenance Treatment With Inhaled Ampicillin in Patients With Cystic Fibrosis and Lung Infection Due to Methicillin-Sensitive Staphylococcus aureus

2012 ◽  
Vol 48 (10) ◽  
pp. 384 ◽  
Author(s):  
Luis Máiz ◽  
Rosa Del Campo ◽  
María Castro ◽  
Dayra Gutiérrez ◽  
Rosa Girón ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Maintenance Treatment ◽  
Lung Infection

scholarly journals Cedecea davisae’s Role in a Polymicrobial Lung Infection in a Cystic Fibrosis Patient

2012 ◽  
Vol 2012 ◽  
pp. 1-4 ◽  
Author(s):  
Thayer G. Ismaael ◽  
Eleana M. Zamora ◽  
Faisal A. Khasawneh
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Burkholderia Cepacia ◽  
Airway Disease ◽  
Lung Infection ◽  
Emerging Pathogen ◽  
The Family ◽  
Healthcare Associated ◽  
Airway Colonization

Chronic airway colonization and infection are the hallmark of cystic fibrosis (CF).Staphylococcus aureus, Pseudomonas aeruginosa, andBurkholderia cepaciaare well-documented bacterial culprits in this chronic suppurative airway disease. Advanced molecular diagnostics have uncovered a possible role of a larger group of microorganisms in CF.Cedeceais a member of the family Enterobacteriaceae and is an emerging pathogen. We present a case of a polymicrobial healthcare-associated pneumonia in a CF patient caused byCedecea davisae, among other bacteria.

Unorthodox long-term aerosolized ampicillin use for methicillin-susceptible Staphylococcus aureus lung infection in a cystic fibrosis patient

2009 ◽  
Vol 44 (5) ◽  
pp. 512-515 ◽  
Author(s):  
Luis Máiz ◽  
Adelaida Lamas ◽  
Ana Fernández-Olmos ◽  
Lucrecia Suárez ◽  
Rafael Cantón
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Cystic Fibrosis Patient ◽  
Lung Infection

Panton-Valentine Leukocidin-Positive Methicillin-Resistant Staphylococcus aureus Lung Infection in Patients With Cystic Fibrosis

CHEST Journal ◽  
2007 ◽  
Vol 131 (6) ◽  
pp. 1718-1725 ◽  
Author(s):  
Arnon Elizur ◽  
Rachel C. Orscheln ◽  
Thomas W. Ferkol ◽  
Jeffrey J. Atkinson ◽  
W. Michael Dunne ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Lung Infection ◽  
Methicillin Resistant ◽  
Panton Valentine Leukocidin ◽  
Valentine Leukocidin

scholarly journals The Staphylococcus aureus Transcriptome during Cystic Fibrosis Lung Infection

mBio ◽  
2019 ◽  
Vol 10 (6) ◽  
Author(s):  
Carolyn B. Ibberson ◽  
Marvin Whiteley
Keyword(s):  
Gene Expression ◽  
Machine Learning ◽  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Lung Infection ◽  
Human Infection ◽  
Content Type ◽  
Bacterial Physiology ◽  
Laboratory Models

ABSTRACT Laboratory models have been invaluable for the field of microbiology for over 100 years and have provided key insights into core aspects of bacterial physiology such as regulation and metabolism. However, it is important to identify the extent to which these models recapitulate bacterial physiology within a human infection environment. Here, we performed transcriptomics (RNA-seq), focusing on the physiology of the prominent pathogen Staphylococcus aureus in situ in human cystic fibrosis (CF) infection. Through principal-component and hierarchal clustering analyses, we found remarkable conservation in S. aureus gene expression in the CF lung despite differences in the patient clinic, clinical status, age, and therapeutic regimen. We used a machine learning approach to identify an S. aureus transcriptomic signature of 32 genes that can reliably distinguish between S. aureus transcriptomes in the CF lung and in vitro. The majority of these genes were involved in virulence and metabolism and were used to improve a common CF infection model. Collectively, these results advance our knowledge of S. aureus physiology during human CF lung infection and demonstrate how in vitro models can be improved to better capture bacterial physiology in infection. IMPORTANCE Although bacteria have been studied in infection for over 100 years, the majority of these studies have utilized laboratory and animal models that often have unknown relevance to the human infections they are meant to represent. A primary challenge has been to assess bacterial physiology in the human host. To address this challenge, we performed transcriptomics of S. aureus during human cystic fibrosis (CF) lung infection. Using a machine learning framework, we defined a “human CF lung transcriptome signature” that primarily included genes involved in metabolism and virulence. In addition, we were able to apply our findings to improve an in vitro model of CF infection. Understanding bacterial gene expression within human infection is a critical step toward the development of improved laboratory models and new therapeutics.

Staphylococcus aureus capsular types and antibody response to lung infection in patients with cystic fibrosis.

1988 ◽  
Vol 26 (12) ◽  
pp. 2505-2509 ◽  
Author(s):  
A Albus ◽  
J M Fournier ◽  
C Wolz ◽  
A Boutonnier ◽  
M Ranke ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Antibody Response ◽  
Lung Infection

scholarly journals Chronic Staphylococcus aureus lung infection correlates with proteogenomic and metabolic adaptations leading to an increased intracellular persistence

2018 ◽  
Author(s):  
Xin Tan ◽  
Mathieu Coureuil ◽  
Elodie Ramond ◽  
Daniel Euphrasie ◽  
Marion Dupuis ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Staphylococcus Aureus ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Lung Infection ◽  
Genetic Modifications ◽  
Protein Expression Profiles ◽  
First Time ◽  
Chronic Lung Infection

AbstractBackgroundChronic lung infection of cystic fibrosis (CF) patients by Staphylococcus aureus is a well-established epidemiological fact. Indeed, S. aureus is the most commonly identified pathogen in the lungs of CF patients. Strikingly the molecular mechanisms underlying S. aureus persistency are not understood.MethodsWe selected pairs of sequential S. aureus isolates from 3 patients with CF and from one patient with non-CF chronic lung disease. We used a combination of genomic, proteomic and metabolomic approaches with functional assays for in-depth characterization of S. aureus long-term persistence.ResultsFor the first time, we show that late S. aureus isolates from CF patients have an increased ability for intracellular survival in CFBE-F508del cells compared to ancestral early isolates. Importantly, the increased ability to persist intracellularly was confirmed for S. aureus isolates within the own patient F508del epithelial cells. An increased ability to form biofilm was also demonstrated.Furthermore, we identified the underlying genetic modifications inducing altered protein expression profiles and notable metabolic changes. These modifications affect several metabolic pathways and virulence regulators that could constitute therapeutic targets.ConclusionsOur results strongly suggest that the intracellular environment might constitute an important niche of persistence and relapse necessitating adapted antibiotic treatments.SummaryS. aureus persists for years in the lungs of patients with cystic fibrosis despite antibiotic therapies. We demonstrate that S. aureus adaptation leads to increased intracellular persistence suggesting a key role for intracellular niche during S. aureus chronic lung infection.

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