Persistence, survival, vertical dispersion, and horizontal spread of the biocontrol agent, Penicillium oxalicum strain 212, in different soil types

2013 ◽  
Vol 67 ◽  
pp. 27-36 ◽  
Author(s):  
Gema Vázquez ◽  
Paloma Melgarejo ◽  
Antonieta De Cal ◽  
Inmaculada Larena
Keyword(s):  
Biocontrol Agent ◽  
Penicillium Oxalicum ◽  
Soil Types ◽  
Vertical Dispersion ◽  
Horizontal Spread

scholarly journals Production, Survival, and Evaluation of Solid-Substrate Inocula of Penicillium oxalicum, a Biocontrol Agent Against Fusarium Wilt of Tomato

2002 ◽  
Vol 92 (8) ◽  
pp. 863-869 ◽  
Author(s):  
I. Larena ◽  
P. Melgarejo ◽  
A. De Cal
Keyword(s):  
Fusarium Wilt ◽  
Biocontrol Agent ◽  
Solid Substrate ◽  
Penicillium Oxalicum ◽  
Conidial Suspension ◽  
Conidial Production ◽  
Plastic Bags ◽  
General Decline ◽  
Leguminous Seeds ◽  
Solid Fermentation

Production of conidia of Penicillium oxalicum (ATCC number pending), a biocontrol agent of Fusarium oxysporum f. sp. lycopersici, was tested in liquid and solid fermentation. P. oxalicum produced 250-fold more conidia in solid than in liquid fermentation at 30 days after inoculation of substrate. Solid fermentation was carried out in plastic bags (600 cm3) especially designed for solid fermentation (VALMIC) containing 50 g of peat/vermiculite (PV) (1:1, wt/wt) with 40% moisture, sealed, sterilized, and then inoculated with 1 ml of a conidial suspension of P. oxalicum (105 conidia g-1 dry substrate), sealed again, and incubated in darkness at 20 to 25°C for 30 days. Addition of amendments to PV in a proportion of 0.5 (wt/wt) significantly increased conidial production of P. oxalicum. The best production was obtained on PV plus meal of cereal grains (barley) or leguminous seeds (lentil) (100-fold higher). Conidial production obtained after 5 days of inoculation was similar to that obtained at 30 days. However, viability of conidia produced in PV plus lentil meal was 35% higher than that of conidia produced in PV plus barley meal. Changes in proportions (1:1:0.5, wt/wt/wt; 1:1:1, wt/wt/wt; 1:0.5:0.5, wt/wt/wt; 1:1:0.5, vol/vol/vol) of components of the substrate (peat/vermiculite/lentil meal) did not enhance production or viability of conidia. Optimal initial moisture in the substrate was 30 to 40%. At lower moistures, significant reductions of production of conidia were observed, particularly at 10%. There was a general decline in the number of conidia in bags with time of storage at -80, -20, 4, and 25°C, or at room temperature (range from 30 to 15°C), with the highest decline occurring from 60 to 180 days. Conidial viability also was reduced with time, except for conidia stored at -20°C. Fresh conidia produced in solid fermentation system or those conidia stored at -20°C for 180 days reduced Fusarium wilt of tomato by 49 and 61%, respectively.

Ecophysiological requirements on growth and survival of the biocontrol agent Penicillium oxalicum 212 in different sterile soils

2014 ◽  
Vol 78 ◽  
pp. 18-27 ◽  
Author(s):  
Inmaculada Larena ◽  
Gema Vázquez ◽  
Antonieta De Cal ◽  
Paloma Melgarejo ◽  
Naresh Magan
Keyword(s):  
Biocontrol Agent ◽  
Penicillium Oxalicum ◽  
Growth And Survival

Abrasive wear of polymer/steel gear drives

2008 ◽  
Vol 4 (1) ◽  
pp. 1-26
Author(s):  
Gábor Kalácska
Keyword(s):  
Wear Resistance ◽  
Abrasive Wear ◽  
Material Selection ◽  
Solid Particles ◽  
Soil Types ◽  
General View ◽  
Modern Engineering ◽  
Clean Environment ◽  
Engineering Polymers ◽  
Plastic Gears

Research was performed on the friction, wear and efficiency of plastic gears made of modern engineering polymers and their composites both in a clean environment (adhesive sliding surfaces) and in an environment contaminated with solid particles and dust (abrasive), with no lubrication at all. The purpose is to give a general view about the results of abrasive wear tests including seven soil types as abrasive media. At the first stage of the research silicious sand was applied between the meshing gears and the wear of plastic and steel gears was evaluated and analyzed from the point of different material properties (elongation at break, hardness, yield stress, modulus of elasticity) and its combinations. The different correlations between the experienced wear and material features are also introduced. At the second stage of the project the abrasive sand was replaced with different physical soil types. The abrasive wear of gears is plotted in the function of soil types. The results highlight on the considerable role of physical soil types on abrasive wear resistance and the conclusions contain the detailed wear resistance. The results offer a new tribology database for the operation and maintenance of agricultural machines with the opportunity of a better material selection according to the dominant soil type. This can finally result longer lifetime and higher reliability of wearing plastic/steel parts.

Effects of heavy metals on soil invertase enzyme activity in different soil types

2010 ◽  
Vol 18 (2) ◽  
pp. 175-180
Author(s):  
Rajesh K. Verma ◽  
Dharam Veer Yadav ◽  
Chandra Pal Singh ◽  
Archna Suman ◽  
Asha Gaur
Keyword(s):  
Heavy Metals ◽  
Enzyme Activity ◽  
Soil Types

Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

2020 ◽  
Vol 13 (2) ◽  
pp. 54-65 ◽  
Author(s):  
M.E.A. Bendaha ◽  
H.A. Belaouni
Keyword(s):  
Root Length ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Enterobacter Ludwigii ◽  
Tomato Growth

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.

What habitat does spiny snout mite occupy in Tasmania

2015 ◽  
Vol 68 ◽  
pp. 446-446
Author(s):  
D.J. Wilson ◽  
P.J. Gerard
Keyword(s):  
New Zealand ◽  
Sand Dune ◽  
Biocontrol Agent ◽  
Predatory Mites ◽  
Mite Species ◽  
Predatory Mite ◽  
Habitat Specificity ◽  
Nontarget Organisms ◽  
Flea Control ◽  
Potential Biocontrol Agent

Spiny snout mite (Neomolgus capillatus) is a potential biocontrol agent for clover flea (Sminthurus viridis) a white clover pest on dairy farms in warmer and wetter parts of New Zealand In the 1990s this mite was introduced from Brittany France into Tasmania for clover flea control Results during the release programme were highly promising and subsequent anecdotal farmer reports indicate widespread decreases in damage As N capillatus is a predatory mite and already known to attack nontarget organisms habitat specificity will determine whether it could be introduced into New Zealand without risk to native insects To assess this pastures on nine of the original Tasmanian release farms and adjacent nontarget habitats ranging from bush wetlands eucalypt stands to sand dune country were sampled in April 2014 Litter samples were collected heat extracted and mite species identified Neomolgus capillatus was found at effective densities in pastures that had good clover cover Where present it displaced Bdellodes spp mites that are ineffective against clover flea No N capillatus were found in the nontarget habitats all of which lacked clover and contained other predatory mites including Bdellodes spp Therefore the preference by N capillatus for lush pastures makes it an excellent prospect for introduction as a biocontrol agent into clover flea prone regions of New Zealand

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