Effectiveness of biosolids in enhancing soil microbial populations and N mineralization in golf course putting greens

2008 ◽  
Vol 40 (2) ◽  
pp. 381-386 ◽  
Author(s):  
G. Tian ◽  
T.C. Granato ◽  
F.D. Dinelli ◽  
A.E. Cox
Keyword(s):  
N Mineralization ◽  
Microbial Populations ◽  
Golf Course ◽  
Putting Greens ◽  
Soil Microbial

scholarly journals Response of Microbial Populations on the Creeping Bentgrass Phyllosphere to Periodic Fungicide Applications

2017 ◽  
Vol 18 (2) ◽  
pp. 44-49
Author(s):  
Joseph R. Doherty ◽  
Megan Botti-Marino ◽  
James P. Kerns ◽  
David F. Ritchie ◽  
Joseph A. Roberts
Keyword(s):  
Creeping Bentgrass ◽  
Weather Conditions ◽  
Deionized Water ◽  
Potato Dextrose Agar ◽  
Microbial Populations ◽  
Golf Course ◽  
Biological Processes ◽  
Putting Greens ◽  
Negative Impacts ◽  
The Impact

Fungicides are frequently applied on golf course putting greens to combat the myriad of diseases that can affect them; however, it is unknown how these fungicides may affect microbial populations. Plant-associated microbial communities are intimately involved with plant development and soil biological processes. This two-year study was designed to evaluate the impact of five selected fungicides on culturable actinomycetes, fungi, general bacteria, and fluorescent pseudomonads residing in the turfgrass phyllosphere. Fungicides were applied at label rates on a 14-day interval. Four samples, consisting of five individual turf plants, were taken five days post-application. Samples were homogenized in 1 ml sterile deionized water and serial diluted to 10−3 and 10−5. Each dilution was then plated onto four different media (actinomycete isolation agar, acidified potato dextrose agar, nutrient agar + 1% sucrose, and King’s B) for enumeration of microbial populations. Fungicides had both positive and negative impacts on the culturable microbes tested, although population shifts across all treatments were observed with the prevailing weather conditions. These results show that while response to repeated fungicide application appears to be organism dependent, microbes are not completely removed from the environment.

Chemical Methods of Moss Control on Golf Course Putting Greens

2005 ◽  
Vol 2 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Brian P. Boesch ◽  
Nathaniel A. Mitkowski
Keyword(s):  
Golf Course ◽  
Chemical Methods ◽  
Putting Greens

Effects of potassium azide on soil microbial populations and soil enzymatic activities

1975 ◽  
Vol 21 (4) ◽  
pp. 565-570 ◽  
Author(s):  
W. D. Kelley ◽  
R. Rodriguez-Kabana
Keyword(s):  
Soil Enzyme ◽  
Soil Microflora ◽  
Microbial Populations ◽  
Bacterial Populations ◽  
Soil Microbial ◽  
Fungal Populations ◽  
Potassium Azide ◽  
Time Changes ◽  
Bacteria And Fungi ◽  
Microbial Numbers

Preplant applications of potassium azide (KN3) to pine nursery beds were evaluated for effect on the soil microflora and on soil enzyme activity where either plastic-sealing or water-sealing techniques were used. Two weeks after incorporation of azide (0–224 kg/ha), soil samplings revealed reduced populations of bacteria and fungi and a corresponding decline in invertase and amylase activities. These effects were proportionate to the amount of azide used and were more pronounced in plastic-sealed plots. Phosphatase activity was little affected. Five weeks after azide application, bacterial populations were higher in treated plots than in controls. Greater numbers of bacteria were recorded from plastic-sealed plots and highest populations coincided with plots receiving the highest rates of azide, regardless of the sealing technique. Fungal populations at this sampling were generally less in treated plots than in the controls, but were higher under plastic seal. At this time, changes in invertase and amylase activities did not correspond to increased microbial numbers. Sixteen weeks after applications of KN3, bacterial populations in treated plots did not differ significantly from controls, but remained higher in plastic-sealed than water-sealed plots. Fungal populations under plastic seal had changed little and remained significantly lower in treated water-sealed plots than in controls. The earlier recorded reduction in invertase and amylase activities was still evident at the final sampling.

scholarly journals Effect of 50 Years of No-Tillage, Stubble Retention, and Nitrogen Fertilization on Soil Respiration, Easily Extractable Glomalin, and Nitrogen Mineralization

Agronomy ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 151
Author(s):  
Pramod Jha ◽  
Kuntal M. Hati ◽  
Ram C. Dalal ◽  
Yash P. Dang ◽  
Peter M. Kopittke ◽  
...  
Keyword(s):  
Soil Respiration ◽  
N Mineralization ◽  
Positive Impact ◽  
N Fertilization ◽  
N Fertilizer ◽  
N Availability ◽  
Soil Microbial ◽  
Stubble Retention ◽  
No Till

In subtropical regions, we have an incomplete understanding of how long-term tillage, stubble, and nitrogen (N) fertilizer management affects soil biological functioning. We examined a subtropical site managed for 50 years using varying tillage (conventional till (CT) and no-till (NT)), stubble management (stubble burning (SB) and stubble retention (SR)), and N fertilization (0 (N0), 30 (N30), and 90 (N90) kg ha−1 y−1) to assess their impact on soil microbial respiration, easily extractable glomalin-related soil protein (EEGRSP), and N mineralization. A significant three-way tillage × stubble × N fertilizer interaction was observed for soil respiration, with NT+SB+N0 treatments generally releasing the highest amounts of CO2 over the incubation period (1135 mg/kg), and NT+SR+N0 treatments releasing the lowest (528 mg/kg). In contrast, a significant stubble × N interaction was observed for both EEGRSP and N mineralization, with the highest concentrations of both EEGRSP (2.66 ± 0.86 g kg−1) and N mineralization (30.7 mg/kg) observed in SR+N90 treatments. Furthermore, N mineralization was also positively correlated with EEGRSP (R2 = 0.76, p < 0.001), indicating that EEGRSP can potentially be used as an index of soil N availability. Overall, this study has shown that SR and N fertilization have a positive impact on soil biological functioning.

scholarly journals Linking Toluene Degradation with Specific Microbial Populations in Soil

1999 ◽  
Vol 65 (12) ◽  
pp. 5403-5408 ◽  
Author(s):  
Jessica R. Hanson ◽  
Jennifer L. Macalady ◽  
David Harris ◽  
Kate M. Scow
Keyword(s):  
Microbial Community ◽  
Microbial Population ◽  
Phospholipid Fatty Acid ◽  
Microbial Populations ◽  
Complex Environments ◽  
Toluene Degradation ◽  
Effective Technique ◽  
Soil Microbial ◽  
Isotope Tracer ◽  
Plfa Analysis

ABSTRACT Phospholipid fatty acid (PLFA) analysis of a soil microbial community was coupled with 13C isotope tracer analysis to measure the community’s response to addition of 35 μg of [13C]toluene ml of soil solution−1. After 119 h of incubation with toluene, 96% of the incorporated13C was detected in only 16 of the total 59 PLFAs (27%) extracted from the soil. Of the total 13C-enriched PLFAs, 85% were identical to the PLFAs contained in a toluene-metabolizing bacterium isolated from the same soil. In contrast, the majority of the soil PLFAs (91%) became labeled when the same soil was incubated with [13C]glucose. Our study showed that coupling13C tracer analysis with PLFA analysis is an effective technique for distinguishing a specific microbial population involved in metabolism of a labeled substrate in complex environments such as soil.

scholarly journals First Report of Subanguina radicicola, the Root-Gall Nematode Infecting Poa annua Putting Greens in Washington State

Plant Disease ◽  
2007 ◽  
Vol 91 (7) ◽  
pp. 905-905 ◽  
Author(s):  
N. A. Mitkowski
Keyword(s):  
United States ◽  
New York ◽  
The United States ◽  
Washington State ◽  
Poa Annua ◽  
Golf Course ◽  
Severe Damage ◽  
Surrounding Water ◽  
Putting Greens ◽  
First Report

In the fall of 2006, a golf course in Snoqualmie, WA renovated five putting greens with commercially produced Poa annua L. sod from British Columbia, Canada. Prior to the renovation, the greens had been planted with Agrostis stolonifera L. cv. Providence, which was removed during the renovation. In February of 2007, chlorotic patches were observed on the newly established P. annua greens. When the roots were examined, extensive galling was observed throughout plant roots. Galls were slender and twisted in appearance and less than one millimeter long. Upon dissection of washed galls, hundreds of eggs were exuded into the surrounding water droplet and both mature male and female nematodes were observed. Further morphometric examination of males, females, and juvenile nematodes demonstrated that they were Subanguina radicicola (Greef 1872) Paramanov 1967 (1). Amplification of nematode 18S, ITS1, and 5.8S regions, using previously published primers (2), resulted in a 100% sequence match with the publicly available sequence for S. radicicola, GenBank Accession No. AF396366. Each P. annua plant had an average of six galls (with a range of 1 to 8), primarily located within the top 2 cm of the soil. All five new P. annua putting greens at the golf course were infested with the nematode. Additionally, P. annua from two A. stolonifera cv. Providence greens that had not been renovated was infected, suggesting that the population occurred onsite and was not imported from the Canadian sod. S. radicicola has been identified as causing severe damage in New Brunswick, Canada on P. annua putting greens and in wild P. annua in the northwestern United States, but to our knowledge, this is the first report of the nematode affecting P. annua on a golf course in the United States. References: (1) E. L. Krall. Wheat and grass nematodes: Anguina, Subanguina, and related genera. Pages 721–760 in: Manual of Agricultural Nematology. Marcel Dekker, New York, 1991. (2) N. A. Mitkowski et al. Plant Dis. 86:840, 2002.

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