Effect of hierarchical porous scaffold on osteoimmunomodulation and bone formation

2020 ◽  
Vol 20 ◽  
pp. 100779
Author(s):  
Yang Liu ◽  
Lingyan Cao ◽  
Shuang Zhang ◽  
Luli Ji ◽  
Jing Wang ◽  
...  
Keyword(s):  
Bone Formation ◽  
Porous Scaffold ◽  
Hierarchical Porous

scholarly journals Hydroxyapatite Block Produced by Sponge Replica Method: Mechanical, Clinical and Histologic Observations

Materials ◽  
2019 ◽  
Vol 12 (19) ◽  
pp. 3079 ◽  
Author(s):  
Antonio Scarano ◽  
Felice Lorusso ◽  
Pablo Santos de Oliveira ◽  
Sanosh Kunjalukkal Padmanabhan ◽  
Antonio Licciulli
Keyword(s):  
Bone Formation ◽  
Porous Scaffold ◽  
Bone Defects ◽  
Replica Method ◽  
Human Model ◽  
Porous Scaffolds ◽  
New Bone Formation ◽  
Maxillary Bone ◽  
Computer Tomography Scan ◽  
Posterior Maxilla

Purpose: The grafting procedure for the anthropic ridges of jaws represents a surgical technique for increasing the bone volume to permit the placement of dental implants for oral rehabilitations. The aim of this study was to evaluate a hydroxyapatite (HA) porous scaffold produced via a sponge replica method for the treatment of maxillary bone defects in a human model. Methods: A total of thirteen patients were treated for sinus lifting in the posterior maxilla for a total of 16 defects treated with cylindrical HA Block. The experimental sites were evaluated by a 3D Cone Beam Computer Tomography scan (CBCT), and the histological analysis was performed after 3 months of healing. Results: After the 3 months healing period, the histological outcome of the investigation showed a high level of biological osteoconduction of the HA. Microscopical evidence of new bone formation was also observed in the central portion of the graft block. The samples were composed of different tissues: 39 ± 1% new bone, 42 ± 3% marrow space, 17 ± 3% residual HA Block and 4.02 ± 2% osteoid tissue were present. The new bone formation in the block was 8 ± 3%. Conclusions: The study findings support that HA porous scaffolds produced by sponge replica were effective for the treatment of maxillary bone defects in humans.

scholarly journals Multiple channels with interconnected pores in a bioceramic scaffold promote bone tissue formation

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Xuesong Wang ◽  
Ziyan Nie ◽  
Jia Chang ◽  
Michael L. Lu ◽  
Yunqing Kang
Keyword(s):  
Bone Formation ◽  
Bone Tissue ◽  
Bone Defect ◽  
Cell Attachment ◽  
Porous Scaffold ◽  
Bone Tissue Regeneration ◽  
Type I ◽  
Multiple Channels ◽  
Filamentous Actin

AbstractInsufficient nutrition exchange and limited transportation of blood supply in a porous only scaffold often hinder bone formation, even though the porous scaffold is loaded with cells or growth factors. To overcome these issues, we developed a cell- and growth factor-free approach to induce bone formation in a critical-size bone defect by using an interconnected porous beta-tricalcium phosphate (β-TCP) scaffold with multiple channels. In vitro cell experimental results showed that multiple channels significantly promoted cell attachment and proliferation of human bone marrow mesenchymal stem cells, stimulated their alkaline phosphatase activity, and up-regulated the osteogenic gene expression. Multiple channels also considerably stimulated the expression of various mechanosensing markers of the cells, such as focal adhesion kinase, filamentous actin, and Yes-associated protein-1 at both static and dynamic culturing conditions. The in vivo bone defect implantation results demonstrated more bone formation inside multiple-channeled scaffolds compared to non-channeled scaffolds. Multiple channels prominently accelerated collagen type I, bone sialoprotein and osteocalcin protein expression. Fluorochrome images and angiogenic marker CD31 staining exhibited more mineral deposition and longer vasculature structures in multiple-channeled scaffolds, compared to non-channeled scaffolds. All the findings suggested that the creation of interconnected multiple channels in the porous β-TCP scaffold is a very promising approach to promote bone tissue regeneration.

Dietary Calcium Supplementation Suppresses Bone Formation in Magnesium-Deficient Rats

2006 ◽  
Vol 76 (3) ◽  
pp. 111-116 ◽  
Author(s):  
Hiroshi Matsuzaki ◽  
Misao Miwa
Keyword(s):  
Bone Formation ◽  
Calcium Supplementation ◽  
Control Diet ◽  
Formation Rate ◽  
Dietary Calcium ◽  
Mineral Apposition Rate ◽  
Control Group ◽  
Deficient Diet ◽  
Bone Formation Rate ◽  
Male Wistar Rats

The purpose of this study was to clarify the effects of dietary calcium (Ca) supplementation on bone metabolism of magnesium (Mg)-deficient rats. Male Wistar rats were randomized by weight into three groups, and fed a control diet (control group), a Mg-deficient diet (Mg- group) or a Mg-deficient diet having twice the control Ca concentrations (Mg-2Ca group) for 14 days. Trabecular bone volume was significantly lower in the Mg - and Mg-2Ca groups than in the control group. Trabecular number was also significantly lower in the Mg - and Mg-2Ca groups than in the control group. Mineralizing bone surface, mineral apposition rate (MAR), and surface referent bone formation rate (BFR/BS) were significantly lower in the Mg - and Mg-2Ca groups than in the control group. Furthermore, MAR and BFR/BS were significantly lower in the Mg-2Ca group than in the Mg - group. These results suggest that dietary Ca supplementation suppresses bone formation in Mg-deficient rats.

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