Effect of resistin on granulosa and theca cell function in cattle

2011 ◽  
Vol 124 (1-2) ◽  
pp. 19-27 ◽  
Author(s):  
Leon J. Spicer ◽  
Nicole B. Schreiber ◽  
Dana V. Lagaly ◽  
Pauline Y. Aad ◽  
Laura B. Douthit ◽  
...  
Keyword(s):  
Cell Function ◽  
Theca Cell

Effects of beta-OH Butyrate on Bovine Granulosa and Theca Cell Function in Vitro

2006 ◽  
Vol 41 (1) ◽  
pp. 39-40 ◽  
Author(s):  
T Vanholder ◽  
JLMR Leroy ◽  
A Soom ◽  
M Coryn ◽  
A Kruif ◽  
...  
Keyword(s):  
Cell Function ◽  
Theca Cell

scholarly journals Effects of angiogenin on granulosa and theca cell function in cattle

animal ◽  
2017 ◽  
Vol 11 (5) ◽  
pp. 811-819 ◽  
Author(s):  
J.L. Dentis ◽  
N.B. Schreiber ◽  
A.M. Burress ◽  
L.J. Spicer
Keyword(s):  
Cell Function ◽  
Theca Cell

Effect of melatonin on bovine theca cells in vitro

2018 ◽  
Vol 30 (4) ◽  
pp. 643 ◽  
Author(s):  
T. Feng ◽  
L. F. Schutz ◽  
B. C. Morrell ◽  
M. C. Perego ◽  
L. J. Spicer
Keyword(s):  
Cell Proliferation ◽  
Cytochrome P450 ◽  
Ovarian Function ◽  
Cell Function ◽  
Regulatory Protein ◽  
Theca Cell ◽  
Theca Cells ◽  
Dependent Inhibition ◽  
Steroidogenic Acute Regulatory

Melatonin affects granulosa cell function in several species but its function in theca cells is less clear, particularly in monotocous animals. Thus, the objectives of this study were to determine the effects of melatonin on theca cell steroidogenesis, gene expression and cell proliferation in a monotocous species, namely cattle. Ovaries were collected from a local bovine abattoir, from which theca cells were isolated from large (8–22 mm) follicles and treated with various hormones in serum-free medium for 24 h or 48 h. Melatonin caused a dose-dependent inhibition (P < 0.05) of LH+insulin-like growth factor 1 (IGF1)-induced androstenedione and progesterone production. Also, melatonin inhibited (P < 0.05) LH+IGF1-induced expression of steroidogenic acute regulatory protein (StAR) mRNA (via real-time polymerase chain reaction) in theca cells, but it had no effect (P > 0.10) on cytochrome P450 11A1 (CYP11A1) and cytochrome P450 17A1 (CYP17A1) mRNA abundance. In LH+IGF1-treated theca cells, melatonin decreased caspase 3 (CASP3) mRNA to levels similar to those observed in LH-treated theca cells. In contrast, melatonin increased (P < 0.05) the number of bovine theca cells in both LH- and LH+IGF1-treated cultures. In conclusion, melatonin may act as an endocrine regulator of ovarian function in cattle by stimulating theca cell proliferation and inhibiting differentiation via inhibition of hormone-induced steroidogenesis.

scholarly journals Assessment of theca cell function prior to controlled ovarian stimulation: the predictive value of serum basal/stimulated steroid levels

2009 ◽  
Vol 25 (1) ◽  
pp. 228-234 ◽  
Author(s):  
J.-N. Hugues ◽  
L. Theron-Gerard ◽  
C. Coussieu ◽  
M. Pasquier ◽  
D. Dewailly ◽  
...  
Keyword(s):  
Ovarian Stimulation ◽  
Predictive Value ◽  
Cell Function ◽  
Theca Cell ◽  
Controlled Ovarian Stimulation

BMP-2 and -6 modulate porcine theca cell function alone and co-cultured with granulosa cells

2005 ◽  
Vol 29 (4) ◽  
pp. 593-604 ◽  
Author(s):  
Victoria Brankin ◽  
Ruth L. Quinn ◽  
Robert Webb ◽  
Morag G. Hunter
Keyword(s):  
Granulosa Cells ◽  
Cell Function ◽  
Theca Cell

Freeze-substitution of rye grass and ragweed pollen grains

1990 ◽  
Vol 48 (3) ◽  
pp. 686-687
Author(s):  
Liza B. Martinez ◽  
Susan M. Wick
Keyword(s):  
Cell Function ◽  
Pollen Grains ◽  
Freeze Substitution ◽  
Cell Types ◽  
Rapid Freezing ◽  
Rye Grass ◽  
Acrylic Resins ◽  
Allergenic Proteins ◽  
Cold Embedding ◽  
Structural Preservation

Rapid freezing and freeze-substitution have been employed as alternatives to chemical fixation because of the improved structural preservation obtained in various cell types. This has been attributed to biomolecular immobilization derived from the extremely rapid arrest of cell function. These methods allow the elimination of conventionally used fixatives, which may have denaturing or “masking” effects on proteins. Thus, this makes them ideal techniques for immunocytochemistry, in which preservation of both ultrastructure and antigenicity are important. These procedures are also compatible with cold embedding acrylic resins which are known to increase sensitivity in immunolabelling.This study reveals how rapid freezing and freeze-substitution may prove to be useful in the study of the mobile allergenic proteins of rye grass and ragweed. Most studies have relied on the use of osmium tetroxide to achieve the necessary ultrastructural detail in pollen whereas those that omitted it have had to contend with poor overall preservation.

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