scholarly journals Hes1 Controls Exocrine Cell Plasticity and Restricts Development of Pancreatic Ductal Adenocarcinoma in a Mouse Model

2016 ◽  
Vol 186 (11) ◽  
pp. 2934-2944 ◽  
Author(s):  
Ana Hidalgo-Sastre ◽  
Roxanne L. Brodylo ◽  
Clara Lubeseder-Martellato ◽  
Bence Sipos ◽  
Katja Steiger ◽  
...  
Keyword(s):  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma ◽  
Cell Plasticity ◽  
Exocrine Cell

408 Requirement for Ikkβ in a Mouse Model of Pancreatic Ductal Adenocarcinoma

2010 ◽  
Vol 138 (5) ◽  
pp. S-61
Author(s):  
Shin Maeda ◽  
Yohko Hikiba ◽  
Kei Sakamoto ◽  
Hayato Nakagawa ◽  
Yoshihiro Hirata ◽  
...  
Keyword(s):  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma

Abstract 1565: β-hydroxy-β-methylbutyrate enhances anti-PD1 immunotherapy in a mouse model of pancreatic ductal adenocarcinoma

2021 ◽  
Author(s):  
Suhas K. Etigunta ◽  
Michael F. Coleman ◽  
Alex J. Pfeil ◽  
Laura M. Lashinger ◽  
Zhengrong Cui ◽  
...  
Keyword(s):  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma

Abstract 2384: A novel mouse model for the study of c-MYC during initiation and progression of pancreatic ductal adenocarcinoma

2011 ◽  
Author(s):  
Wan-Chi Lin ◽  
Qian Zhang ◽  
Rene Opavsky ◽  
Dominick J. DiMaio ◽  
Dean W. Felsher ◽  
...  
Keyword(s):  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma

scholarly journals Acinar transformed ductal cells exhibit differential mucin expression in a tamoxifen-induced pancreatic ductal adenocarcinoma mouse model

Biology Open ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. bio052878
Author(s):  
Kavita Mallya ◽  
Dhanya Haridas ◽  
Parthasarathy Seshacharyulu ◽  
Ramesh Pothuraju ◽  
Wade M. Junker ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Intraepithelial Neoplasia ◽  
Ductal Adenocarcinoma ◽  
Chemotherapy Response ◽  
Pancreatic Intraepithelial Neoplasia ◽  
Ductal Cells ◽  
Mucin Expression ◽  
Acinar To Ductal Metaplasia

ABSTRACTPancreatic cancer (PC) is acquired postnatally; to mimic this scenario, we developed an inducible KrasG12D; Ptf1a-CreER™ (iKC) mouse model, in which Kras is activated postnatally at week 16 upon tamoxifen (TAM) administration. Upon TAM treatment, iKC mice develop pancreatic intraepithelial neoplasia (PanIN) lesions and PC with metastasis at the fourth and fortieth weeks, respectively, and exhibited acinar-to-ductal metaplasia (ADM) and transdifferentiation. Kras activation upregulated the transcription factors Ncoa3, p-cJun and FoxM1, which in turn upregulated expression of transmembrane mucins (Muc1, Muc4 and Muc16) and secretory mucin (Muc5Ac). Interestingly, knockdown of KrasG12D in multiple PC cell lines resulted in downregulation of MUC1, MUC4, MUC5AC and MUC16. In addition, iKC mice exhibited ADM and transdifferentiation. Our results show that the iKC mouse more closely mimics human PC development and can be used to investigate pancreatic ductal adenocarcinoma (PDAC) biomarkers, early onset of PDAC, and ADM. The iKC model can also be used for preclinical strategies such as targeting mucin axis alone or in combination with neo-adjuvant, immunotherapeutic approaches and to monitor chemotherapy response.

scholarly journals MicroRNA-21 Is Induced Early in Pancreatic Ductal Adenocarcinoma Precursor Lesions

2010 ◽  
Vol 56 (4) ◽  
pp. 603-612 ◽  
Author(s):  
Maël Chalret du Rieu ◽  
Jérôme Torrisani ◽  
Janick Selves ◽  
Talal Al Saati ◽  
Anny Souque ◽  
...  
Keyword(s):  
Early Detection ◽  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Early Stage ◽  
Survival Rates ◽  
Ductal Adenocarcinoma ◽  
Early Event ◽  
Precursor Lesions ◽  
Tissue Samples ◽  
Ductal Cells

Abstract Background: Pancreatic ductal adenocarcinoma (PDAC) has the poorest overall prognosis among gastrointestinal cancers; however, curative resection in early-stage PDAC greatly improves survival rates, indicating the importance of early detection. Because abnormal microRNA production is commonly detected in cancer, we investigated noninvasive precursor pancreatic intraepithelial neoplasia (PanIN) lesions for microRNA production as a potential early biomarker of PDAC. Methods: Pathologists identified and classified ductal lesions. We extracted total RNA from laser-capture microdissected PanIN tissue samples from a conditional KRAS(G12D) mouse model (n = 29) or of human origin (n = 38) (KRAS is v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog). MicroRNA production was quantified by quantitative real-time PCR. Internal controls included 5S and U6 RNAs. Results: Production of microRNAs miR-21, miR-205, and miR-200 paralleled PanIN progression in the KRAS(G12D) mouse model, compared with microRNA production in samples of nonpathologic ducts. miR-21 demonstrated the highest relative concentrations in the precursor lesions. Interestingly, miR-205 and miR-21 up-regulation preceded phenotypic changes in the ducts. The production of microRNAs miR-21, miR-221, miR-222, and let-7a increased with human PanIN grade, with peak production occurring in hyperplastic PanIN-2/3 lesions. In situ hybridization analysis indicated miR-21 production to be concentrated in pathologic ductal cells. miR-21 production was regulated by KRAS(G12D) and epidermal growth factor receptor in PDAC-derived cell lines. Conclusions: Aberrant microRNA production is an early event in the development of PanIN. Our findings indicate that miR-21 warrants further investigation as a marker for early detection of PDAC.

A mouse model for pain and neuroplastic changes associated with pancreatic ductal adenocarcinoma

Pain ◽  
2017 ◽  
Vol 158 (8) ◽  
pp. 1609-1621 ◽  
Author(s):  
Deepitha Selvaraj ◽  
Michael Hirth ◽  
Jagadeesh Gandla ◽  
Rohini Kuner
Keyword(s):  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Ductal Adenocarcinoma

scholarly journals Tumor-reducing effect of the clinically used drug clofazimine in a SCID mouse model of pancreatic ductal adenocarcinoma

Oncotarget ◽  
2016 ◽  
Vol 8 (24) ◽  
pp. 38276-38293 ◽  
Author(s):  
Angela Zaccagnino ◽  
Antonella Managò ◽  
Luigi Leanza ◽  
Artur Gontarewitz ◽  
Bernhard Linder ◽  
...  
Keyword(s):  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Scid Mouse ◽  
Ductal Adenocarcinoma ◽  
Scid Mouse Model
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