scholarly journals 89Zr-PET imaging of DNA double-strand breaks for the early monitoring of response following α- and β-particle radioimmunotherapy in a mouse model of pancreatic ductal adenocarcinoma

Theranostics ◽  
2020 ◽  
Vol 10 (13) ◽  
pp. 5802-5814
Author(s):  
Sophie Poty ◽  
Komal Mandleywala ◽  
Edward O'Neill ◽  
James C. Knight ◽  
Bart Cornelissen ◽  
...  
Keyword(s):  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Pet Imaging ◽  
Double Strand Breaks ◽  
Ductal Adenocarcinoma ◽  
Dna Double Strand Breaks ◽  
Strand Breaks

scholarly journals PET Imaging of DNA Damage Response following 225Ac-radioimmunotherapy in a Pancreatic Ductal Adenocarcinoma Mouse Model

2019 ◽  
Vol 50 (1) ◽  
pp. S4-S5
Author(s):  
Sophie Poty ◽  
James C. Knight ◽  
Patrick Donabedian ◽  
Komal Mandleywala ◽  
Bart Cornelissen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Dna Damage Response ◽  
Pet Imaging ◽  
Ductal Adenocarcinoma ◽  
Damage Response

PET Imaging of DNA Damage Response following 225Ac-radioimmunotherapy in a Pancreatic Ductal Adenocarcinoma Mouse Model

2019 ◽  
Vol 50 (4) ◽  
pp. S70
Author(s):  
Sophie Poty ◽  
James C. Knight ◽  
Patrick Donabedian ◽  
Komal Mandleywala ◽  
Bart Cornelissen ◽  
...  
Keyword(s):  
Dna Damage ◽  
Mouse Model ◽  
Pancreatic Ductal Adenocarcinoma ◽  
Dna Damage Response ◽  
Pet Imaging ◽  
Ductal Adenocarcinoma ◽  
Damage Response

Repair pathway choice for double-strand breaks

2020 ◽  
Vol 64 (5) ◽  
pp. 765-777 ◽  
Author(s):  
Yixi Xu ◽  
Dongyi Xu
Keyword(s):  
Cell Cycle ◽  
Double Strand Breaks ◽  
Homologous Region ◽  
Gene Repair ◽  
Repair Pathway ◽  
Dna Double Strand Breaks ◽  
Environmental Radiation ◽  
Strand Breaks ◽  
Dna End Resection ◽  
End Resection

Abstract Deoxyribonucleic acid (DNA) is at a constant risk of damage from endogenous substances, environmental radiation, and chemical stressors. DNA double-strand breaks (DSBs) pose a significant threat to genomic integrity and cell survival. There are two major pathways for DSB repair: nonhomologous end-joining (NHEJ) and homologous recombination (HR). The extent of DNA end resection, which determines the length of the 3′ single-stranded DNA (ssDNA) overhang, is the primary factor that determines whether repair is carried out via NHEJ or HR. NHEJ, which does not require a 3′ ssDNA tail, occurs throughout the cell cycle. 53BP1 and the cofactors PTIP or RIF1-shieldin protect the broken DNA end, inhibit long-range end resection and thus promote NHEJ. In contrast, HR mainly occurs during the S/G2 phase and requires DNA end processing to create a 3′ tail that can invade a homologous region, ensuring faithful gene repair. BRCA1 and the cofactors CtIP, EXO1, BLM/DNA2, and the MRE11–RAD50–NBS1 (MRN) complex promote DNA end resection and thus HR. DNA resection is influenced by the cell cycle, the chromatin environment, and the complexity of the DNA end break. Herein, we summarize the key factors involved in repair pathway selection for DSBs and discuss recent related publications.

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