scholarly journals Far Upstream Element Binding Protein Plays a Crucial Role in Embryonic Development, Hematopoiesis, and Stabilizing Myc Expression Levels

2016 ◽  
Vol 186 (3) ◽  
pp. 701-715 ◽  
Author(s):  
Weixin Zhou ◽  
Yang Jo Chung ◽  
Edgardo R. Parrilla Castellar ◽  
Ying Zheng ◽  
Hye-Jung Chung ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Crucial Role ◽  
Binding Protein ◽  
Expression Levels ◽  
Element Binding Protein ◽  
Upstream Element

scholarly journals Anti-IL-17 Antibody Improves Hepatic Steatosis by Suppressing Interleukin-17-Related Fatty Acid Synthesis and Metabolism

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Weidong Shi ◽  
Qiang Zhu ◽  
Jian Gu ◽  
Xiaoshan Liu ◽  
Ling Lu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Liver Disease ◽  
Hepatic Steatosis ◽  
Fatty Acid Metabolism ◽  
Alcoholic Liver Disease ◽  
Acid Metabolism ◽  
Binding Protein ◽  
Interleukin 17 ◽  
Expression Levels ◽  
Element Binding Protein

To investigate the relationship between interleukin-17 and proteins involved in fatty acid metabolism with respect to alcoholic liver disease, male ICR mice were randomized into five groups: control, alcoholic liver disease (ALD) at 4 weeks, 8 weeks, and 12 weeks, and anti-IL-17 antibody treated ALD. A proteomic approach was adopted to investigate changes in liver proteins between control and ALD groups. The proteomic analysis was performed by two-dimensional difference gel electrophoresis. Spots of interest were subsequently subjected to nanospray ionization tandem mass spectrometry (MS/MS) for protein identification. Additionally, expression levels of selected proteins were confirmed by western blot. Transcriptional levels of some selected proteins were determined by RT-PCR. Expression levels of 95 protein spots changed significantly (ratio >1.5,P<0.05) during the development of ALD. Sterol regulatory element-binding protein-lc (SREBP-1c), carbohydrate response element binding protein (ChREBP), enoyl-coenzyme A hydratase (ECHS1), and peroxisome proliferator-activated receptor alpha (PPAR-α) were identified by MS/MS among the proteins shown to vary the most; increased IL-17 elevated the transcription of SREBP-1c and ChREBP but suppressed ECHS1 and PPAR-α. The interleukin-17 signaling pathway is involved in ALD development; anti-IL-17 antibody improved hepatic steatosis by suppressing interleukin-17-related fatty acid metabolism.

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