scholarly journals Human Lung Cancer–Derived Immunosuppressive Plasmacytoid Dendritic Cells Release IL-1α in an AIM2 Inflammasome-Dependent Manner

2015 ◽  
Vol 185 (11) ◽  
pp. 3115-3124 ◽  
Author(s):  
Rosalinda Sorrentino ◽  
Michela Terlizzi ◽  
Vincenzo G. Di Crescenzo ◽  
Ada Popolo ◽  
Michela Pecoraro ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dendritic Cells ◽  
Human Lung ◽  
Plasmacytoid Dendritic Cells ◽  
Human Lung Cancer ◽  
Dependent Manner

Plasmacytoid Dendritic Cells from Human Lung Cancer Draining Lymph Nodes Induce Tc1 Responses

2007 ◽  
Vol 36 (3) ◽  
pp. 360-367 ◽  
Author(s):  
Alexander Faith ◽  
Emma Peek ◽  
Joanne McDonald ◽  
Zoe Urry ◽  
David F. Richards ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dendritic Cells ◽  
Lymph Nodes ◽  
Human Lung ◽  
Plasmacytoid Dendritic Cells ◽  
Human Lung Cancer ◽  
Draining Lymph Nodes

scholarly journals Cytotoxic Effects of Flubendazole in Human Lung Cancer Cell Line A549

2020 ◽  
Vol 11 (4) ◽  
Author(s):  
Elham Hoveizi ◽  
Fatemeh Fakharzadeh Jahromi
Keyword(s):  
Lung Cancer ◽  
Cell Viability ◽  
Human Lung ◽  
A549 Cells ◽  
Beclin 1 ◽  
Human Lung Cancer ◽  
Pcr Analysis ◽  
Dependent Manner ◽  
Concentration Dependent Manner ◽  
Acridine Orange Staining

Background: The development of effective anticancer drugs is a significant health issue. Previous studies showed that members of the benzimidazole family have anticancer effects on several cancers Objectives: The present study investigated the cytotoxic effect of flubendazole on A549 human lung cancer cells. Methods: The A549 cells were treated with flubendazole at 1, 2, 5, and 10 µM concentrations for three days. Cell viability was measured by the MTT assay and Acridine orange staining. Also, the expressions of P62 and Beclin -1 were analyzed by qRT-PCR analysis. Results: Cell viability of A549 cells, in a concentration-dependent manner, showed significant differences between the treatment and control groups, and the IC50 value was determined to be 2 µM. Also, flubendazole reduced the expression of P62 and increased the expression of Beclin 1 in treated cells. Conclusions: Flubendazole induces cell death in A549 cells in a dose and time-dependent manner and can offer new factors in lung cancer therapeutic strategies.

scholarly journals Morphological Changes in H1299 Human Lung Cancer Cells Following Millimeter-Wave Irradiation

2020 ◽  
Author(s):  
Konstantin Komoshvili ◽  
Tzippi Beker ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
Ayan Barbora ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Side Effects ◽  
Cancer Cells ◽  
Human Lung ◽  
Morphological Changes ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Physical Parameters ◽  
Dependent Manner ◽  
Human Lung Cancer Cells

Efficiently targeted cancer therapy without causing detrimental side effects is necessary for alleviating patient care and improving survival rates. This paper presents observations of morphological changes in H1299 human lung cancer cells following MMW irradiation (75 – 105 GHz) at a non-thermal power density of 0.2 mW/cm2, investigated over 14 days of subsequent physiological incubation following exposure. Microscopic analyses of physical parameters measured indicate MMW irradiation induces significant morphological changes characteristic of apoptosis and senescence. The Immediate short-term stress responses translate into long-term effects, retained over the duration of the experiment(s); reminiscent of the phenomenon of Accelerated Cellular Senescence (ACS) achieving terminal tumorigenic cell growth. Further, results were observed to be treatment-specific in energy (dose) dependent manner and were achieved without the use of chemotherapeutic agents, ionizing radiation or thermal ablation employed in conventional methods; thereby overcome associated side effects. Adaptation of the experimental parameters of this study in clinical oncology concomitant with current developmental trends of non-invasive medical endoscopy alleviates MMW therapy as an effective treatment procedure for human non-small cell lung cancer (NSLC)

scholarly journals A bombesin receptor subtype-3 peptide increases nuclear oncogene expression in a MEK-1 dependent manner in human lung cancer cells

2001 ◽  
Vol 412 (1) ◽  
pp. 13-20 ◽  
Author(s):  
H.Christian Weber ◽  
James Walters ◽  
Julius Leyton ◽  
Marchessini Casibang ◽  
Sally Purdom ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Human Lung ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Receptor Subtype ◽  
Dependent Manner ◽  
Human Lung Cancer Cells ◽  
Bombesin Receptor ◽  
Subtype 3

scholarly journals Morphological Changes in H1299 Human Lung Cancer Cells Following Millimeter-Wave Irradiation

2020 ◽  
Author(s):  
Konstantin Komoshvili ◽  
Tzippi Becker ◽  
Jacob Levitan ◽  
Asher Yahalom ◽  
Ayan Barbora ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Side Effects ◽  
Cancer Cells ◽  
Human Lung ◽  
Morphological Changes ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Physical Parameters ◽  
Dependent Manner ◽  
Human Lung Cancer Cells

Efficiently targeted cancer therapy without causing detrimental side effects is necessary for alleviating patient care and improving survival rates. This paper presents observations of morphological changes in H1299 human lung cancer cells following W-band MMW irradiation (75 – 105 GHz) at a non-thermal power density of 0.2 mW/cm2, investigated over 14 days of subsequent physiological incubation following exposure. Microscopic analyses of physical parameters measured indicate MMW irradiation induces significant morphological changes characteristic of apoptosis and senescence. The Immediate short-term responses translate into long-term effects, retained over the duration of the experiment(s); reminiscent of the phenomenon of Accelerated Cellular Senescence (ACS) achieving terminal tumorigenic cell growth. Further, results were observed to be treatment-specific in energy (dose) dependent manner and were achieved without the use of chemotherapeutic agents, ionizing radiation or thermal ablation employed in conventional methods; thereby overcoming associated side effects. Adaptation of the experimental parameters of this study for clinical oncology concomitant with current developmental trends of non-invasive medical endoscopy alleviates MMW therapy as an effective treatment procedure for human non-small cell lung cancer (NSCLC).

scholarly journals Furanocoumarin A: A Novel Anticancer Agent on Human Lung Cancer A549 Cells from Fructus liquidambaris

2020 ◽  
Vol 19 (17) ◽  
pp. 2091-2096 ◽  
Author(s):  
Hui Fang ◽  
Hongmei Ji
Keyword(s):  
Lung Cancer ◽  
Ethyl Acetate ◽  
Column Chromatography ◽  
Anticancer Agent ◽  
Human Lung ◽  
Water Solution ◽  
A549 Cells ◽  
Human Lung Cancer ◽  
Dependent Manner ◽  
Alcohol Water

Background and Purpose: The fruit of Fructus liquidambaris, which is recently being used for cancer treatment, has a history to be used as a traditional medicine in China for thousands of years. Material and Methods: Ten kg of dried F. liquidambaris was obtained with 70% alcohol-water solution under reflux for three times. The condensed extract was obtained from petroleum ether, ethyl acetate and N-butyl alcohol, respectively. Ethyl acetate extract was subjected to silica gel column, Sephadex LH-20, ODS column chromatography and RP-HPLC column chromatography to yield a new compound (1). The structure was identified through intensive analysis of NMR and MS spectra. The antitumor mechanism of the furanocoumarin A on human lung cancer A549 cells was confirmed by detecting the apoptosis-related proteins. Result: Furanocoumarin A (1), a novel furanocoumarin constituent was isolated and identified from F. Liquidambaris. The IC50 value of furanocoumarin A on A549 cell lines was 65.28±5.36µM obtained by the method of MTT. The compound could induce the apoptosis of A549 cells by inducing 21.5% early apoptosis and 32.4% late apoptosis at the concentration of 60µmol/L. Western blot analysis indicated that protein expressions of p53, caspase 3 and Bax increased in a dose-dependent manner between the concentrations from 40 to 80µM. The protein expression of Bcl-2 decreased the concentration of 60 and 80μM. The ratio of Bcl-2 to Bax was inversely proportional to the dose concentration. Conclusion: Furanocoumarin A could be a novel anticancer agent from herbal medicine.

scholarly journals Sinomenine Inhibits Migration and Invasion of Human Lung Cancer Cell through Downregulating Expression of miR-21 and MMPs

2020 ◽  
Vol 21 (9) ◽  
pp. 3080 ◽  
Author(s):  
Kun-Hung Shen ◽  
Jui-Hsiang Hung ◽  
Yi-Ching Liao ◽  
Shu-Ting Tsai ◽  
Ming-Jiuan Wu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Matrix Metalloproteinase ◽  
Cell Invasion ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Matrix Metalloproteinase 2 ◽  
Migration And Invasion ◽  
Dependent Manner ◽  
Mesenchymal Transition ◽  
E Cadherin

Sinomenine is an alkaloid derived from Sinomenium acutum. Recent studies have found that sinomenine can inhibit various cancers by inhibiting the proliferation, migration and invasion of tumors and inducing apoptosis. This study aims to investigate the effect and mechanism of sinomenine on inhibiting the migration and invasion of human lung adenocarcinoma cells in vitro. The results demonstrate that viabilities of A549 and H1299 cells were inhibited by sinomenine in a dose-dependent manner. When treated with sub-toxic doses of sinomenine, cell migration and invasion are markedly suppressed. Sinomenine decreases the mRNA level of matrix metalloproteinase-2 (MMP-2), MMP-9, and the extracellular inducer of matrix metalloproteinase (EMMPRIN/CD147), but elevates the expression of reversion-inducing cysteine-rich proteins with kazal motifs (RECK) and the tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2. In addition, sinomenine significantly increases the expression of the epithelial marker E-cadherin but concomitantly decreases the expression of the mesenchymal marker vimentin, suggesting that it suppresses epithelial–mesenchymal transition (EMT). Moreover, sinomenine downregulates oncogenic microRNA-21 (miR-21), which has been known to target RECK. The downregulation of miR-21 decreases cell invasion, while the upregulation of miR-21 increases cell invasion. Furthermore, the downregulation of miR-21 stimulates the expression of RECK, TIMP-1/-2, and E-cadherin, but reduces the expression of MMP-2/-9, EMMPRIN/CD147, and vimentin. Taken together, the results reveal that the inhibition of A549 cell invasion by sinomenine may, at least in part, be through the downregulating expression of MMPs and miR-21. These findings demonstrate an attractive therapeutic potential for sinomenine in lung cancer anti-metastatic therapy.

scholarly journals Antitumor Activity of Lentivirus-mediated Interleukin -12 Gene Modified Dendritic Cells in Human Lung Cancer in Vitro

2014 ◽  
Vol 15 (2) ◽  
pp. 611-616 ◽  
Author(s):  
Hassan Abdellah Ahmed Ali ◽  
Jun Di ◽  
Wu Mei ◽  
Yu-Cheng Zhang ◽  
Yi Li ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Dendritic Cells ◽  
Antitumor Activity ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Interleukin 12

scholarly journals ZAK inhibits human lung cancer cell growth via ERK and JNK activation in an AP-1-dependent manner

2010 ◽  
Vol 101 (6) ◽  
pp. 1374-1381 ◽  
Author(s):  
Jaw-Ji Yang ◽  
Yi-Ju Lee ◽  
Hsin-Hung Hung ◽  
Wei-Pu Tseng ◽  
Chuan-Chou Tu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Human Lung ◽  
Human Lung Cancer ◽  
Dependent Manner ◽  
Lung Cancer Cell ◽  
Cancer Cell Growth ◽  
Human Lung Cancer Cell ◽  
Jnk Activation

Extracts from the Roots of Lindera strychifolia Induces Apoptosis in Lung Cancer Cells and Prolongs Survival of Tumor-bearing Mice

2003 ◽  
Vol 31 (06) ◽  
pp. 857-869 ◽  
Author(s):  
Yun-Mo Li ◽  
Yasushi Ohno ◽  
Shinya Minatoguchi ◽  
Kazunori Fukuda ◽  
Tetsuro Ikoma ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Oral Administration ◽  
Tumor Cell ◽  
Cancer Cells ◽  
Human Lung ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Dependent Manner ◽  
Dose Dependent

Lindera strychifolia, a scandent shrub Lauraceous medicinal plant, has been used in Chinese traditional medicine as a palliative and an anti-spasmodic. It also shows cytotoxic effects against several tumor cell lines and inhibits marcromolecule biosynthesis. This study investigated the anti-tumor effects of L. strychifolia extract against lung cancer cells using in vitro and in vivo models. Two human lung cancer cell lines A549 (adenocarcinoma) and SBC-3 (small cell carcinoma), and a non-tumor cell line 3T3-L1 (mice fibroblasts) were subjected to L. strychifolia extract treatment. On lung cancer cells, L. strychifolia induced cell growth inhibition in a dose-dependent manner. Conversely, the extract did not show any significant cytotoxic effect on 3T3-L1 cells. Therefore, the extract is specific for tumor cells. Tumor cells treated with L. strychifolia extract showed typical morphological appearance of apoptosis including nuclei fragmentation and cell condensation. The in vivo effects of L. strychifolia extract were investigated in C57BL/6 mice transplanted with Lewis lung cancer (LL-2) cells, and in BALB/c nude mice transplanted with A549 or SBC-3 human lung cancer cells. Oral administration of L. strychifolia extract prolonged survival time and inhibited tumor growth in a dose-dependent manner by inducing apoptosis in the LL-2 cell mice model. Furthermore, in A549 or SBC-3 cell nude mice models, oral administration of L. strychifolia extract also significantly inhibited tumor growth at the 5.0 mg/ml concentration. These findings suggested that the components of L. strychifolia have anticancer activity and may contribute to clinical applications in the prevention and treatment of lung cancer.

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