scholarly journals Methionine Adenosyltransferase 2B–GIT1 Complex Serves as a Scaffold to Regulate Ras/Raf/MEK1/2 Activity in Human Liver and Colon Cancer Cells

2015 ◽  
Vol 185 (4) ◽  
pp. 1135-1144 ◽  
Author(s):  
Hui Peng ◽  
Tony W.H. Li ◽  
Heping Yang ◽  
Mary P. Moyer ◽  
Jose M. Mato ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Methionine Adenosyltransferase ◽  
Colon Cancer Cells

scholarly journals Insulin-like growth factor 1 activates methionine adenosyltransferase 2A transcription by multiple pathways in human colon cancer cells

2011 ◽  
Vol 436 (2) ◽  
pp. 507-516 ◽  
Author(s):  
Heping Yang ◽  
Tony W. H. Li ◽  
Jian Peng ◽  
José M. Mato ◽  
Shelly C. Lu
Keyword(s):  
Colon Cancer ◽  
Growth Factor ◽  
Protein Binding ◽  
Cancer Cells ◽  
Promoter Activity ◽  
Human Colon ◽  
Methionine Adenosyltransferase ◽  
Insulin Like Growth Factor ◽  
Colon Cancer Cells ◽  
Human Colon Cancer

We have previously reported that the expression of MAT2A (methionine adenosyltransferase 2A) is increased in human colon cancer and in colon cancer cells treated with IGF-1 (insulin-like growth factor-1), which was required for its mitogenic effect. The aim of the present study was to elucidate the molecular mechanisms of IGF-1-mediated MAT2A induction. Nuclear run-on analysis confirmed that the increase in MAT2A expression lies at the transcriptional level. DNase I footprinting of the MAT2A promoter region revealed a similar protein-binding pattern in colon cancer and IGF-1-treated RKO cells. IGF-1 induced MAT2A promoter activity and increased nuclear protein binding to USF (upstream stimulatory factor)/c-Myb, YY1 (Yin and Yang 1), E2F, AP-1 (activator protein 1) and NF-κB (nuclear factor κB) consensus elements. IGF-1 increased the expression of c-Jun, FosB, MafG, p65, c-Myb, E2F-1 and YY1 at the pre-translational level. Knockdown of p65, MafG, c-Myb or E2F-1 lowered basal MAT2A expression and blunted the inductive effect of IGF-1 on MAT2A, whereas knockdown of YY1 increased basal MAT2A expression and had no effect on IGF-1-mediated MAT2A induction. Consistently, mutation of AP-1, NF-κB, E2F and USF/c-Myb elements individually blunted the IGF-1-mediated increase in MAT2A promoter activity, and combined mutations completely prevented the increase. In conclusion, IGF-1 activates MAT2A transcription by both known and novel pathways. YY1 represses MAT2A expression.

scholarly journals Oleocanthal exerts antitumor effects on human liver and colon cancer cells through ROS generation

2017 ◽  
Vol 51 (2) ◽  
pp. 533-544 ◽  
Author(s):  
Antonella Cusimano ◽  
Daniele Balasus ◽  
Antonina Azzolina ◽  
Giuseppa Augello ◽  
Maria R. Emma ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Ros Generation ◽  
Colon Cancer Cells ◽  
Antitumor Effects

scholarly journals Effect of 5-azacytidine (5-aza) on UCP2 expression in human liver and colon cancer cells

2021 ◽  
Vol 18 (10) ◽  
pp. 2176-2186
Author(s):  
Dae-Yeon Kim ◽  
Hee-Tae Cheong ◽  
Chang-Six Ra ◽  
Kazuhiro Kimura ◽  
Bae Dong Jung
Keyword(s):  
Colon Cancer ◽  
Cancer Cells ◽  
Human Liver ◽  
Colon Cancer Cells ◽  
Ucp2 Expression

Leptin is a growth factor for human colon cancer cells

2001 ◽  
Vol 120 (5) ◽  
pp. A493-A493
Author(s):  
J HARDWICK ◽  
G VANDENBRINK ◽  
S VANDEVENTER ◽  
M PEPPELENBOSCH
Keyword(s):  
Colon Cancer ◽  
Growth Factor ◽  
Cancer Cells ◽  
Human Colon ◽  
Colon Cancer Cells ◽  
Human Colon Cancer ◽  
Human Colon Cancer Cells
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