scholarly journals In vitro cardiac tissue models: Current status and future prospects

2016 ◽  
Vol 96 ◽  
pp. 203-213 ◽  
Author(s):  
Anurag Mathur ◽  
Zhen Ma ◽  
Peter Loskill ◽  
Shaheen Jeeawoody ◽  
Kevin E. Healy
Keyword(s):  
Cardiac Tissue ◽  
Current Status ◽  
Future Prospects ◽  
Tissue Models

Biophysical stimulation for in vitro engineering of functional cardiac tissues

2017 ◽  
Vol 131 (13) ◽  
pp. 1393-1404 ◽  
Author(s):  
Anastasia Korolj ◽  
Erika Yan Wang ◽  
Robert A. Civitarese ◽  
Milica Radisic
Keyword(s):  
Cardiac Tissue ◽  
Culture Media ◽  
Culture Conditions ◽  
Lineage Specification ◽  
Cardiac Tissues ◽  
Cardiac Lineage ◽  
And Function ◽  
Tissue Models

Engineering functional cardiac tissues remains an ongoing significant challenge due to the complexity of the native environment. However, our growing understanding of key parameters of the in vivo cardiac microenvironment and our ability to replicate those parameters in vitro are resulting in the development of increasingly sophisticated models of engineered cardiac tissues (ECT). This review examines some of the most relevant parameters that may be applied in culture leading to higher fidelity cardiac tissue models. These include the biochemical composition of culture media and cardiac lineage specification, co-culture conditions, electrical and mechanical stimulation, and the application of hydrogels, various biomaterials, and scaffolds. The review will also summarize some of the recent functional human tissue models that have been developed for in vivo and in vitro applications. Ultimately, the creation of sophisticated ECT that replicate native structure and function will be instrumental in advancing cell-based therapeutics and in providing advanced models for drug discovery and testing.

scholarly journals HPMC Capsules: Current Status and Future Prospects

2010 ◽  
Vol 13 (3) ◽  
pp. 428 ◽  
Author(s):  
Moawia M Al-Tabakha
Keyword(s):  
Hydroxypropyl Methylcellulose ◽  
In Vitro Dissolution ◽  
Current Status ◽  
Modified Release ◽  
Future Prospects ◽  
Gelatin Capsules ◽  
Hpmc Capsules ◽  
Hard Gelatin Capsules

Hydroxypropyl methylcellulose (HPMC) is employed for a wide variety of pharmaceutical and food preparations. Its applications as viscolizing agent (thickening agent), coating polymer, bioadhesive, in solid dispersion to enhance solubility, binder in the process of granulation and in modified release formulations have been well documented. One other notable use is in the production of capsule shells, replacing the animal derived gelatin in conventional two-piece capsules. The aim of this review is to systemically survey published literature on the HPMC use in capsule shells and resolve questions regarding their suitability as a replacement for hard gelatin capsules. Future refinements in the production and filling of HPMC capsule shells and improvement in their in vivo/in vitro dissolution would ensure their superiority over hard gelatin capsules.

scholarly journals Generation of functional human hepatocytes in vitro: current status and future prospects

2019 ◽  
Vol 39 (1) ◽  
Author(s):  
Tomoko Yamaguchi ◽  
Juntaro Matsuzaki ◽  
Takeshi Katsuda ◽  
Yoshimasa Saito ◽  
Hidetsugu Saito ◽  
...  
Keyword(s):  
Human Hepatocytes ◽  
Current Status ◽  
Future Prospects

scholarly journals Proteases and Their Inhibitors in Chronic Obstructive Pulmonary Disease

2018 ◽  
Vol 7 (9) ◽  
pp. 244 ◽  
Author(s):  
Tapan Dey ◽  
Jatin Kalita ◽  
Sinéad Weldon ◽  
Clifford Taggart
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Drug Development ◽  
Pulmonary Disease ◽  
Current Status ◽  
Chronic Obstructive ◽  
Future Prospects ◽  
Obstructive Pulmonary Disease ◽  
Drug Molecules

In the context of respiratory disease, chronic obstructive pulmonary disease (COPD) is the leading cause of mortality worldwide. Despite much development in the area of drug development, currently there are no effective medicines available for the treatment of this disease. An imbalance in the protease: Antiprotease ratio in the COPD lung remains an important aspect of COPD pathophysiology and several studies have shown the efficacy of antiprotease therapy in both in vitro and in vivo COPD models. However more in-depth studies will be required to validate the efficacy of lead drug molecules targeting these proteases. This review discusses the current status of protease-directed drugs used for treating COPD and explores the future prospects of utilizing the potential of antiprotease-based therapeutics as a treatment for this disease.

Engineered Microtissues for Real-Time Characterization of Cardiomyocyte Function

2013 ◽  
Author(s):  
Ariane C. C. van Spreeuwel ◽  
Noortje A. M. Bax ◽  
Jasper Foolen ◽  
M. A. Borochin ◽  
Daisy W. J. van der Schaft ◽  
...  
Keyword(s):  
Real Time ◽  
Cardiac Tissue ◽  
Mechanical Performance ◽  
Diagnostic Tools ◽  
Cardiac Physiology ◽  
Surrounding Matrix ◽  
Speed Up ◽  
Tissue Models

Engineered cardiac tissue models become increasingly important for understanding normal and disease cardiac physiology [1]. Where clinical diagnostic tools usually measure overall function of the heart, cardiac tissue models make it possible to focus on single CMs and their microenvironment. The use of in-vitro cardiac disease models can give more insight in the functionality changes of CMs during disease and thereby speed up the development of new therapies. Therefore, we aim to develop a model for healthy and diseased myocardium to study the effect of diseased microenvironments on the mechanical performance of CMs. The platform consists of 3D engineered microtissues with matrix, CMs and fibroblasts (FBs) on an array of polydimethylsiloxane (PDMS) microposts and allows for real-time characterization of CMs and their surrounding matrix. The design was adapted from Legant et. al. [2] and enables us to measure inhomogeneous tissue forces which may occur if not all cells contract equally. Here we focus on optimization and validation of the platform to measure contraction forces and gain insight in CM mechanical functioning.

scholarly journals Rapid 3D bioprinting of in vitro cardiac tissue models using human embryonic stem cell-derived cardiomyocytes

Bioprinting ◽  
2019 ◽  
Vol 13 ◽  
pp. e00040 ◽  
Author(s):  
Justin Liu ◽  
Jingjin He ◽  
Jingfeng Liu ◽  
Xuanyi Ma ◽  
Qu Chen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Embryonic Stem Cell ◽  
Human Embryonic Stem Cell ◽  
Cardiac Tissue ◽  
Embryonic Stem ◽  
3D Bioprinting ◽  
Tissue Models
Sign in / Sign up

Export Citation Format

Share Document