scholarly journals A cocktail of growth factors released from a heparin hyaluronic-acid hydrogel promotes the myogenic potential of human urine-derived stem cells in vivo

2020 ◽  
Vol 107 ◽  
pp. 50-64 ◽  
Author(s):  
Guihua Liu ◽  
Rongpei Wu ◽  
Bin Yang ◽  
Yingai Shi ◽  
Chunhua Deng ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Growth Factors ◽  
Human Urine ◽  
Myogenic Potential ◽  
Hyaluronic Acid Hydrogel

scholarly journals mRNA-Driven Generation of Transgene-Free Neural Stem Cells from Human Urine-Derived Cells

Cells ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 1043 ◽  
Author(s):  
Phil Jun Kang ◽  
Daryeon Son ◽  
Tae Hee Ko ◽  
Wonjun Hong ◽  
Wonjin Yun ◽  
...  
Keyword(s):  
Stem Cells ◽  
Neural Stem Cells ◽  
Human Urine ◽  
Neurological Disorders ◽  
Therapeutic Potential ◽  
Embryonic Stem ◽  
Global Gene Expression ◽  
Patient Specific ◽  
Human Neural Stem Cells

Human neural stem cells (NSCs) hold enormous promise for neurological disorders, typically requiring their expandable and differentiable properties for regeneration of damaged neural tissues. Despite the therapeutic potential of induced NSCs (iNSCs), a major challenge for clinical feasibility is the presence of integrated transgenes in the host genome, contributing to the risk for undesired genotoxicity and tumorigenesis. Here, we describe the advanced transgene-free generation of iNSCs from human urine-derived cells (HUCs) by combining a cocktail of defined small molecules with self-replicable mRNA delivery. The established iNSCs were completely transgene-free in their cytosol and genome and further resembled human embryonic stem cell-derived NSCs in the morphology, biological characteristics, global gene expression, and potential to differentiate into functional neurons, astrocytes, and oligodendrocytes. Moreover, iNSC colonies were observed within eight days under optimized conditions, and no teratomas formed in vivo, implying the absence of pluripotent cells. This study proposes an approach to generate transplantable iNSCs that can be broadly applied for neurological disorders in a safe, efficient, and patient-specific manner.

scholarly journals Biotechnology Applied to Cosmetics and Aesthetic Medicines

Cosmetics ◽  
2020 ◽  
Vol 7 (2) ◽  
pp. 33
Author(s):  
Cátia Gomes ◽  
Ana Catarina Silva ◽  
Ana Camila Marques ◽  
José Sousa Lobo ◽  
Maria Helena Amaral
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Growth Factors ◽  
Genetic Engineering ◽  
Kojic Acid ◽  
Active Ingredients ◽  
High Quality ◽  
Vast Number ◽  
Biotechnological Processes

Biotechnology uses microorganisms and/or enzymes to obtain specific products through fermentative processes and/or genetic engineering techniques. Examples of these products are active ingredients, such as hyaluronic acid, kojic acid, resveratrol, and some enzymes, which are used in skin anti-aging products. In addition, certain growth factors, algae, stem cells, and peptides have been included in cosmetics and aesthetic medicines. Thus, biotechnology, cosmetics and aesthetic medicines are now closely linked, through the production of high-quality active ingredients, which are more effective and safer. This work describes the most used active ingredients that are produced from biotechnological processes. Although there are a vast number of active ingredients, the number of biotechnological active ingredients reported in the literature is not significantly high.

TRENDS AND CHALLENGES OF CARTILAGE TISSUE ENGINEERING

2009 ◽  
Vol 21 (03) ◽  
pp. 149-155 ◽  
Author(s):  
Hsu-Wei Fang
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Growth Factors ◽  
Bone Cells ◽  
Cartilage Tissue Engineering ◽  
Bioactive Molecules ◽  
In Vivo Studies ◽  
Cartilage Tissue

Cartilage injuries may be caused by trauma, biomechanical imbalance, or degenerative changes of joint. Unfortunately, cartilage has limited capability to spontaneous repair once damaged and may lead to progressive damage and degeneration. Cartilage tissue-engineering techniques have emerged as the potential clinical strategies. An ideal tissue-engineering approach to cartilage repair should offer good integration into both the host cartilage and the subchondral bone. Cells, scaffolds, and growth factors make up the tissue engineering triad. One of the major challenges for cartilage tissue engineering is cell source and cell numbers. Due to the limitations of proliferation for mature chondrocytes, current studies have alternated to use stem cells as a potential source. In the recent years, a lot of novel biomaterials has been continuously developed and investigated in various in vitro and in vivo studies for cartilage tissue engineering. Moreover, stimulatory factors such as bioactive molecules have been explored to induce or enhance cartilage formation. Growth factors and other additives could be added into culture media in vitro, transferred into cells, or incorporated into scaffolds for in vivo delivery to promote cellular differentiation and tissue regeneration.Based on the current development of cartilage tissue engineering, there exist challenges to overcome. How to manipulate the interactions between cells, scaffold, and signals to achieve the moderation of implanted composite differentiate into moderate stem cells to differentiate into hyaline cartilage to perform the optimum physiological and biomechanical functions without negative side effects remains the target to pursue.

Combination of Hyaluronic Acid Hydrogel Scaffold and PLGA Microspheres for Supporting Survival of Neural Stem Cells

2011 ◽  
Vol 28 (6) ◽  
pp. 1406-1414 ◽  
Author(s):  
Ying Wang ◽  
Yue Teng Wei ◽  
Zhao Hui Zu ◽  
Rong Kai Ju ◽  
Mu Yao Guo ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Neural Stem Cells ◽  
Plga Microspheres ◽  
Hydrogel Scaffold ◽  
Hyaluronic Acid Hydrogel

Hyaluronic Acid, PRP/Growth Factors, and Stem Cells in the Treatment of Osteochondral Lesions

2017 ◽  
pp. 659-677
Author(s):  
Diana Ribeiro Pereira ◽  
Joana Silva-Correia ◽  
Elisaveta Kon ◽  
Hélder Pereira ◽  
Piero Volpi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Hyaluronic Acid ◽  
Growth Factors ◽  
Osteochondral Lesions

G.P.16.10 In vivo myogenic potential of human AC133 muscle-derived stem cells

2007 ◽  
Vol 17 (9-10) ◽  
pp. 877
Author(s):  
E. Negroni ◽  
I. Riederer ◽  
J. Di Santo ◽  
Y. Torrente ◽  
V. Mouly ◽  
...  
Keyword(s):  
Stem Cells ◽  
Myogenic Potential

Fibroblast Growth Factors Regulate Stem Cell Functioning In Vitro and in Vivo.

Blood ◽  
2004 ◽  
Vol 104 (11) ◽  
pp. 1705-1705
Author(s):  
Joyce S.G Yeoh ◽  
Ronald van Os ◽  
Ellen Weersing ◽  
Bert Dontje ◽  
Edo Vellenga ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Growth Factors ◽  
Cultured Cells ◽  
Cell Activity ◽  
Fibroblast Growth Factors ◽  
Fibroblast Growth ◽  
Stem Cell Activity

Abstract Fibroblast Growth Factors (FGF) are a large family of signaling molecules widely involved in tissue development, maintenance and repair. Little is known about the role of FGF/FGF-receptor signaling in the regulation of adult hematopoietic stem cells (HSC). In this study, we assessed the potential of exogenously added FGF-1/2, or retrovirally overexpressed FGF-1 to preserve HSC function in vitro and in vivo. First, we demonstrate that in vitro culture of unfractionated mouse bone marrow cells, in serum-free medium, supplemented with FGF-1 or FGF-2 or FGF-1 + 2 resulted in the robust generation of long-term repopulating (LTR) HSCs. Cultures were maintained for 12 weeks and during that time in vivo competitive reconstitution assays were performed. Stem cell activity was detectable at 3, 5, and 8 weeks after initiation of culture, but lost after 12 weeks. However, whereas 3 and 5 week cultured cells provided radioprotection in non-competitive assays, animals transplanted with 8 or 12 week cultured cells succumbed due to bone marrow failure. So far, we have been unable to expand single, highly purified Lin−Sca-1+c-Kit+ using FGF-1 + 2. Consequently, we speculated that essential intermediate cell populations or signals are required for FGF-induced stem cell conservation. To test this we cultured highly purified CD45.1 Lin−Sca-1+c-Kit+ cells in a co-culture with CD45.2 unfractionated BM. Co-cultured cells were transplanted after 5 weeks in lethally irradiated recipients, and CD45.1 chimerism levels were assessed. High levels of CD45.1 chimerism confirmed that Lin−Sca-1+c-Kit+ cells require an accessory signal in addition to FGF to induced stem cell activity in vitro. We subsequently tested stem cell potential of cells cultured in FGF-1 + 2 for 5 weeks, with the addition of SCF + IL-11 + Flt3L for the last 2, 4 or 7 days. Cell numbers increased with increasing time of growth factor presence. However, only when growth factors were present for 2 days engraftment of cultured cells in a competitive repopulation assay was increased 3.5-fold. Finally, we show by immunohistochemistry that ~10% of freshly isolated Lin−Sca-1+c-Kit+ expresses high levels of FGF-1. Retroviral overexpression of FGF-1 in stem cells resulted in increased growth potential and sustained clonogenic activity in vitro. Upon transplantation of transduced stem cells, FGF-1 overexpression resulted in increased white blood cell counts 4 weeks post-transplant compared to control animals. Most notable was a marked granulocytosis in FGF-1 overexpressing recipients Our results reveal FGF as an important regulator of HSC signaling and homeostasis. Importantly, in the presence of FGF stem cells can be maintained in vitro for 2 months. These findings open novel avenues for in vitro manipulation of stem cells for future clinical therapies.

Novel Technique for Isolating Human Bone Marrow Stem Cells Using Hyaluronic Acid Hydrogel

2016 ◽  
Vol 22 (10) ◽  
pp. 941-951 ◽  
Author(s):  
Jung-Seok Lee ◽  
Seul-Ki Kim ◽  
Jae-Kook Cha ◽  
Byung-Joo Jung ◽  
Seong-Bok Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hyaluronic Acid ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Novel Technique ◽  
Hyaluronic Acid Hydrogel

Mechanical Stimulation by Ultrasound Enhances Chondrogenic Differentiation of Mesenchymal Stem Cells in a Fibrin-Hyaluronic Acid Hydrogel

2013 ◽  
Vol 37 (7) ◽  
pp. 648-655 ◽  
Author(s):  
Jae Won Choi ◽  
Byung Hyune Choi ◽  
Sang-Hyug Park ◽  
Ki Soo Pai ◽  
Tian Zhu Li ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Mechanical Stimulation ◽  
Chondrogenic Differentiation ◽  
Hyaluronic Acid Hydrogel
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