Novel Technique for Isolating Human Bone Marrow Stem Cells Using Hyaluronic Acid Hydrogel

2016 ◽  
Vol 22 (10) ◽  
pp. 941-951 ◽  
Author(s):  
Jung-Seok Lee ◽  
Seul-Ki Kim ◽  
Jae-Kook Cha ◽  
Byung-Joo Jung ◽  
Seong-Bok Choi ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Hyaluronic Acid ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Novel Technique ◽  
Hyaluronic Acid Hydrogel

scholarly journals Hyaluronic acid/poly-l-lysine bilayered silica nanoparticles enhance the osteogenic differentiation of human mesenchymal stem cells

2014 ◽  
Vol 2 (40) ◽  
pp. 6939-6946 ◽  
Author(s):  
Sara Amorim ◽  
Albino Martins ◽  
Nuno M. Neves ◽  
Rui L. Reis ◽  
Ricardo A. Pires
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Hyaluronic Acid ◽  
Osteogenic Differentiation ◽  
Silica Nanoparticles ◽  
Human Mesenchymal Stem Cells ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow

The coating of silica nanoparticles with a bilayer of poly-l-lysine and hyaluronic acid enhances the osteogenic differentiation of human bone marrow stem cells at low nanoparticle concentrations (25 μg mL−1 and 12.5 μg mL−1).

scholarly journals Human dental pulp stem cells exhibit enhanced properties in comparison to human bone marrow stem cells on neurites outgrowth

2020 ◽  
Vol 34 (4) ◽  
pp. 5499-5511 ◽  
Author(s):  
Pierfrancesco Pagella ◽  
Shayee Miran ◽  
Estrela Neto ◽  
Ivan Martin ◽  
Meriem Lamghari ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dental Pulp ◽  
Dental Pulp Stem Cells ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Dental Pulp ◽  
Enhanced Properties

scholarly journals The Role of Mitochondria in the Dual Effect of Low-Temperature Plasma on Human Bone Marrow Stem Cells: From Apoptosis to Activation of Cell Proliferation

2020 ◽  
Vol 10 (24) ◽  
pp. 8971
Author(s):  
Sergej V. Belov ◽  
Yakov P. Lobachevsky ◽  
Yurij K. Danilejko ◽  
Aleksej B. Egorov ◽  
Alexander V. Simakin ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Low Temperature ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Low Temperature Plasma ◽  
Dual Effect ◽  
Temperature Plasma

The potential use of low-temperature plasma (LTP) for therapeutic purposes has aroused the concern of many researchers. This paper examines the effect of LTP on the morphofunctional state of human bone marrow stem cells (BMSC). It has been established that LTP-induced oxidative stress has a dual effect on the state of stem cells. On the one hand, a cell culture exposed to LTP exhibits the progression of a destructive processes, which is manifested by the perturbation of the cell’s morphology, the initiation of lipid peroxidation and the accumulation of products of this process, like diene conjugates and malondialdehyde, as well as the induction of mitochondrial dysfunction, leading to cell death. On the other hand, the effect of LTP on BMSC located at a distance from the plasma is accompanied by the activation of proliferative processes, as evidenced by the tendency of the activity of mitochondrial biogenesis and fission/fusion processes to increase. The paper discusses the role of mitochondria and reactive oxygen species (ROS) in the cellular response to LTP.

scholarly journals Concentrated Growth Factors (CGF) Induce Osteogenic Differentiation in Human Bone Marrow Stem Cells

Biology ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 370
Author(s):  
Alessio Rochira ◽  
Luisa Siculella ◽  
Fabrizio Damiano ◽  
Andrea Palermo ◽  
Franco Ferrante ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Osteogenic Differentiation ◽  
Bone Marrow Stem Cells ◽  
Protein Quantification ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Osteogenic Medium ◽  
Alizarin Red ◽  
Matrix Mineralization

Bone regeneration is a complex process regulated by several factors that control overlapping biological processes, coordinating interactions among distinct cell populations. There is a great interest in identifying new strategies for inducing osteogenesis in a safe and efficient manner. Concentrated Growth Factor (CGF) is an autologous blood derived product obtained by centrifugation of venous blood following the procedure set on the Silfradent device. In this study the effects of CGF on osteogenic differentiation of human Bone Marrow Stem Cells (hBMSC) in vitro have been investigated; hBMSC were cultured with CGF or osteogenic medium, for 21 days. The osteogenic differentiation was evaluated measuring alkaline phosphatase (ALP) enzyme activity, matrix mineralization by alizarin red staining and through mRNA and protein quantification of osteogenic differentiation markers by Real-time PCR and Western blotting, respectively. The treatment with CGF stimulated ALP activity and promoted matrix mineralization compared to control and seems to be more effective than osteogenic medium. Also, hBMSC lost mesenchymal markers and showed other osteogenic features. Our study showed for the first time that CGF alone is able to induce osteogenic differentiation in hBMSC. The application of CGF on hBMSC osteoinduction might offer new clinical and biotechnological strategies in the tissue regeneration field.

Human bone marrow stem cells co-cultured with neonatal rat cardiomyocytes display limited cardiomyogenic plasticity

Cytotherapy ◽  
2009 ◽  
pp. 1-15 ◽  
Author(s):  
Remco Koninckx ◽  
Karen Hensen ◽  
Annick Daniëls ◽  
Marjan Moreels ◽  
Ivo Lambrichts ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Neonatal Rat ◽  
Human Bone Marrow ◽  
Rat Cardiomyocytes ◽  
Neonatal Rat Cardiomyocytes

scholarly journals The Effects of Extracellular pH on Proliferation and Differentiation of human Bone Marrow Stem Cells

2012 ◽  
Vol 19 (1) ◽  
pp. 35 ◽  
Author(s):  
Yea Hyun Leem ◽  
Tae Suk Nam ◽  
Jung Hwa Kim ◽  
Kang Sik Lee ◽  
Dong Ho Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Extracellular Ph ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Proliferation And Differentiation

Cellular methods in cartilage research: Primary human chondrocytes in culture and chondrogenesis in human bone marrow stem cells

Methods ◽  
2008 ◽  
Vol 45 (1) ◽  
pp. 2-9 ◽  
Author(s):  
Simon R. Tew ◽  
Alan D. Murdoch ◽  
Richard P. Rauchenberg ◽  
Timothy E. Hardingham
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Bone Marrow Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Human Chondrocytes
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