scholarly journals Histone-targeted gene transfer of bone morphogenetic protein-2 enhances mesenchymal stem cell chondrogenic differentiation

2018 ◽  
Vol 71 ◽  
pp. 156-167 ◽  
Author(s):  
Erik V. Munsell ◽  
Deepa S. Kurpad ◽  
Theresa A. Freeman ◽  
Millicent O. Sullivan
Keyword(s):  
Stem Cell ◽  
Gene Transfer ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Chondrogenic Differentiation ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein

scholarly journals Mesenchymal Stem Cell-mediated Gene Delivery of Bone Morphogenetic Protein-2 in an Articular Fracture Model

2007 ◽  
Vol 15 (8) ◽  
pp. 1543-1550 ◽  
Author(s):  
Terri Zachos ◽  
Alisha Diggs ◽  
Steven Weisbrode ◽  
Jeffrey Bartlett ◽  
Alicia Bertone
Keyword(s):  
Stem Cell ◽  
Gene Delivery ◽  
Mesenchymal Stem Cell ◽  
Bone Morphogenetic Protein ◽  
Fracture Model ◽  
Bone Morphogenetic Protein 2 ◽  
Articular Fracture ◽  
Morphogenetic Protein

scholarly journals Umbilical Cord Mesenchymal Stem Cell-Derived Nanovesicles Potentiate the Bone-Formation Efficacy of Bone Morphogenetic Protein 2

2020 ◽  
Vol 21 (17) ◽  
pp. 6425
Author(s):  
Songhyun Lim ◽  
Hao-Zhen Lyu ◽  
Ju-Ro Lee ◽  
Shi Huan Han ◽  
Jae Hyup Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Bone Formation ◽  
Bone Morphogenetic Protein ◽  
Umbilical Cord ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Calvarial Defects ◽  
In Vivo Bone Formation

Recombinant human bone morphogenetic protein 2 (rhBMP-2) is one of the most potent osteogenic factors used to treat bone loss. However, at higher doses, rhBMP-2 does not necessarily increase bone formation but rather increases the incidence of adverse side effects. Here, we investigated whether umbilical cord mesenchymal stem cell (UCMSC)-derived nanovesicles (NVs) further increase the in vivo bone formation at high doses of rhBMP-2. In the presence of UCMSC-derived NVs, proliferation, migration, and tube formation of human umbilical vein endothelial cells were stimulated in vitro. Furthermore, migration and osteogenesis of human bone marrow-derived mesenchymal stem cells were stimulated. To examine the efficacy of UCMSC-derived NVs on in vivo bone formation, collagen sponges soaked with rhBMP-2 and UCMSC-derived NVs were used in athymic nude mice with calvarial defects. At a high rhBMP-2 dosage (500 ng/mL), UCMSC-derived NVs significantly promoted bone formation in calvarial defects; however, the UCMSC-derived NVs alone did not induce in vivo bone formation. Our results indicate that UCMSC-derived NVs can potentiate the bone formation efficacy of rhBMP-2 at a high dosage.

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