scholarly journals The influence of biomimetic topographical features and the extracellular matrix peptide RGD on human corneal epithelial contact guidance

2013 ◽  
Vol 9 (2) ◽  
pp. 5040-5051 ◽  
Author(s):  
E.J. Tocce ◽  
S.J. Liliensiek ◽  
A.H. Broderick ◽  
Y. Jiang ◽  
K.C. Murphy ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Contact Guidance ◽  
Corneal Epithelial ◽  
Peptide Rgd

scholarly journals Stimulation of corneal differentiation by interaction between cell surface and extracellular matrix. I. Morphometric analysis of transfilter "induction".

1975 ◽  
Vol 66 (2) ◽  
pp. 275-291 ◽  
Author(s):  
L Meier ◽  
E D Hay
Keyword(s):  
Extracellular Matrix ◽  
Electron Microscope ◽  
Cell Surface ◽  
Pore Size ◽  
Collagen Synthesis ◽  
Lens Capsule ◽  
Physical Contact ◽  
Corneal Epithelial ◽  
Cell Processes

The present study was undertaken to determine whether or not physical contact with the substratum is essential for the stimulatory effect of extracellular matrix (ECM) on corneal epithelial collagen synthesis. Previous studies showed that collagenous substrata stimulate isolated epithelia to produce three times as much collagen as they produce on noncollagenous substrate; killed collagenous substrata (e.g., lens capsule) are just as effective as living substrata (e.g., living lens) in promoting the production of new corneal stroma in vitro. In the experiments to be reported here, corneal epithelia were placed on one side of Nucleopore filters of different pore sizes and killed lens capsule on the other, with the expectation that contact of the reacting cells with the lens ECM should be limited by the number and size of the cell processes that can tranverse the pores. Transfilter cultures were grown for 24 h in [3H]proline-containing median and incorporation of isotope into hot trichloroacetic acid-soluble protein was used to measure corneal epithelial collagen production. Epithelial collagen synthesis increases directly as the size of the pores in the interposed filter increases and decreases as the thickness of the filter layer increases. Cell processes within Nucleopore filters were identified with the transmission electron microscope with difficulty; with the scanning electron microscope, however, the processes could easily be seen emerging from the undersurface of even 0.1-mum pore size filters. Morphometric techniques were used to show that cell surface area thus exposed to the underlying ECM is linearly correlated with enhancement of collagen synthesis. Epithelial cell processes did not pass through ultrathin (25-mum thick) 0.45-mum pore size Millipore filters nor did "induction" occur across them. The results are discussed in relation to current theories of embryonic tissue interaction.

Inhibition of Human Corneal Epithelial Production of Fibrotic Mediator TGF-β2 by Basement Membrane–Like Extracellular Matrix

2007 ◽  
Vol 48 (3) ◽  
pp. 1061 ◽  
Author(s):  
Adriana J. LaGier ◽  
Sonia H. Yoo ◽  
Eduardo C. Alfonso ◽  
Sally Meiners ◽  
M. Elizabeth Fini
Keyword(s):  
Extracellular Matrix ◽  
Basement Membrane ◽  
Corneal Epithelial

scholarly journals Dynamics of 3D carcinoma cell invasion into aligned collagen

2018 ◽  
Vol 10 (2) ◽  
pp. 100-112 ◽  
Author(s):  
Arja Ray ◽  
Rachel K. Morford ◽  
Nima Ghaderi ◽  
David J. Odde ◽  
Paolo. P. Provenzano
Keyword(s):  
Extracellular Matrix ◽  
Cancer Cell ◽  
Cell Invasion ◽  
Carcinoma Cell ◽  
Spatiotemporal Dynamics ◽  
Contact Inhibition ◽  
Contact Guidance ◽  
Cell Behavior ◽  
Cancer Cell Invasion ◽  
Invasive Front

We present a novel platform to quantify spatiotemporal dynamics of cell behavior at and beyond the invasive front and demonstrate that contact inhibition and contact guidance orchestrate cancer cell invasion into anisotropic extracellular matrix.

scholarly journals Involvement of YAP, TAZ and HSP90 in Contact Guidance and Intercellular Junction Formation in Corneal Epithelial Cells

PLoS ONE ◽  
2014 ◽  
Vol 9 (10) ◽  
pp. e109811 ◽  
Author(s):  
Vijay Krishna Raghunathan ◽  
Britta Dreier ◽  
Joshua T. Morgan ◽  
Binh C. Tuyen ◽  
Brad W. Rose ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Intercellular Junction ◽  
Contact Guidance ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Junction Formation

scholarly journals Influence of Extracellular Matrix Proteins and Substratum Topography on Corneal Epithelial Cell Alignment and Migration

2013 ◽  
Vol 19 (15-16) ◽  
pp. 1713-1722 ◽  
Author(s):  
VijayKrishna Raghunathan ◽  
Clayton McKee ◽  
Wai Cheung ◽  
Rachel Naik ◽  
Paul F. Nealey ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cell ◽  
Extracellular Matrix Proteins ◽  
Matrix Proteins ◽  
Corneal Epithelial Cell ◽  
Cell Alignment ◽  
Corneal Epithelial ◽  
And Migration

Role of glycosaminoglycans and collagen in the development of a fibronectin-rich extracellular matrix in cultured embryonic corneal epithelial cells

1984 ◽  
Vol 67 (1) ◽  
pp. 189-202
Author(s):  
D.L. Mattey ◽  
D.R. Garrod
Keyword(s):  
Extracellular Matrix ◽  
Epithelial Cells ◽  
Immunofluorescent Staining ◽  
Corneal Epithelial Cells ◽  
Corneal Epithelial ◽  
Collagen Secretion ◽  
The Matrix ◽  
Coated Surface ◽  
Marginal Cells ◽  
Cells Cultured

Corneal epithelial cells from 15-day chick embryos produce a fibronectin-rich extracellular matrix when cultured on glass, plastic and fibronectin-coated substrata. Cell culture in the presence of Streptomyces hyaluronidase or chondroitinase ABC resulted in considerable reduction of the matrix; collagenase had a lesser effect but nevertheless also reduced the matrix. In all enzyme treatments the cells attached and spread to form characteristic epithelial cell islands, but the marginal cells of these islands showed a marked reduction in the number of lamellipodia and focal contacts. Also, the immunofluorescent staining pattern for fibronectin was considerably reduced. Control cells cultured on a fibronectin-coated surface were able to reorganize the fibronectin into fibrils, whereas cells cultured in enzymes showed little or no ability to do so. The cellular reorganization of fibronectin could also be inhibited by the addition of L-azetidine-2-carboxylic acid (LACA), an inhibitor of collagen secretion. Cells plated out in the presence of LACA spread much better on collagen substrata than on plastic, glass or fibronectin. However, in all cases very little fibronectin matrix was detectable in the epithelial islands. The results suggest that components of the extracellular matrix (ECM) such as collagen, hyaluronic acid and chondroitin sulphates are not essential for the initial attachment and spreading of corneal epithelial cells in culture, but are important in the development of the ECM, and in maintaining a flattened morphology and spreading behaviour. It is suggested that fibronectin plays an important role in these interactions and that the ability of cells to organize fibronectin into fibrils is dependent on the presence of other ECM components such as glycosaminoglycans and collagen.

Contact guidance induced organization of extracellular matrix

Biomaterials ◽  
2004 ◽  
Vol 25 (17) ◽  
pp. 3631-3638 ◽  
Author(s):  
Michael E. Manwaring ◽  
Jennifer F. Walsh ◽  
Patrick A. Tresco
Keyword(s):  
Extracellular Matrix ◽  
Contact Guidance

Neural tissue engineering: the influence of scaffold surface topography and extracellular matrix microenvironment

2021 ◽  
Author(s):  
Chun-Yi Yang ◽  
Wei-Yuan Huang ◽  
Liang-Hsin Chen ◽  
Nai-Wen Liang ◽  
Huan-Chih Wang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Extracellular Matrix ◽  
Surface Topography ◽  
Neural Tissue ◽  
Contact Guidance ◽  
Neural Tissue Engineering

Strategies using surface topography, contact guidance and biomechanical cues in the design of scaffolds as an ECM support for neural tissue engineering.

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