scholarly journals Novel silk fibroin/elastin wound dressings

2012 ◽  
Vol 8 (8) ◽  
pp. 3049-3060 ◽  
Author(s):  
Andreia Vasconcelos ◽  
Andreia C. Gomes ◽  
Artur Cavaco-Paulo
Keyword(s):  
Silk Fibroin ◽  
Wound Dressings

scholarly journals Silk derived formulations for accelerated wound healing in diabetic mice

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10232
Author(s):  
Muniba Tariq ◽  
Hafiz Muhammad Tahir ◽  
Samima Asad Butt ◽  
Shaukat Ali ◽  
Asma Bashir Ahmad ◽  
...  
Keyword(s):  
Wound Healing ◽  
Silk Fibroin ◽  
Chronic Wounds ◽  
Histological Analysis ◽  
Healing Process ◽  
Aloe Vera ◽  
Wound Contraction ◽  
Wound Dressings ◽  
Control Group ◽  
Diabetic Mice

Background The present study aimed to prepare effective silk derived formulations in combination with plant extract (Aloe vera gel) to speed up the wound healing process in diabetic mice. Methods Diabetes was induced in albino mice by using alloxan monohydrate. After successful induction of diabetes in mice, excision wounds were created via biopsy puncture (6 mm). Wound healing effect of silk sericin (5%) and silk fibroin (5%) individually and in combination with 5% Aloe vera gel was evaluated by determining the percent wound contraction, healing time and histological analysis. Results The results indicated that the best biocompatible silk combination was of 5% silk fibroin and 5% Aloe vera gel in which wounds were healed in 13 days with wound contraction: 98.33 ± 0.80%. In contrast, the wound of the control group (polyfax) healed in 19 day shaving 98.5 ± 0.67% contraction. Histological analysis revealed that the wounds which were treated with silk formulations exhibited an increased growth of blood vessels, collagen fibers, and much reduced inflammation. Conclusion It can be concluded that a combination of Bombyx mori silk and Aloe vera gel is a natural biomaterial that can be utilized in wound dressings and to prepare more innovative silk based formulations for speedy recovery of chronic wounds.

scholarly journals Photocrosslinking silver nanoparticles–aloe vera–silk fibroin composite hydrogel for treatment of full-thickness cutaneous wounds

2021 ◽  
Vol 8 (6) ◽  
Author(s):  
Yangkun Liu ◽  
JinChuan Fan ◽  
MingQi Lv ◽  
Kepeng She ◽  
Jiale Sun ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Silver Nanoparticles ◽  
Silk Fibroin ◽  
Aloe Vera ◽  
Antibacterial Properties ◽  
Composite Hydrogel ◽  
Wound Dressings ◽  
Full Thickness ◽  
Cutaneous Wounds

Abstract Damage to the skin causes physiological and functional issues. The most effective treatment approach is the use of wound dressings. Silk fibroin (SF) is a promising candidate biomaterial for regulating wound healing; however, its antibacterial properties and biological activity must be further improved. In this study, a photocrosslinking hydrogel was developed to treat full-thickness cutaneous wounds. The composite hydrogel (Ag–AV–SF hydrogel) was prepared by introducing the silver nanoparticles (AgNPs) and aloe vera (AV) as the modifiers. In vitro study exhibited great antibacterial ability, biocompatibility and cell-proliferation and -migration-promoting capacities. It also showed the pH-response releasing properties which release more AgNPs in a simulated chronic infection environment. The healing effect evaluation in vivo showed the healing-promoting ability of the Ag–AV–SF hydrogel was stronger than the single-modifiers groups, and the healing rate of it reached 97.02% on Day 21, higher than the commercial wound dressing, silver sulfadiazine (SS) cream on sale. Additionally, the histological and protein expression results showed that the Ag–AV–SF hydrogel has a greater effect on the pro-healing regenerative phenotype with M2 macrophages at the early stage, reconstructing the blood vessels networks and inhibiting the formation of scars. In summary, the Ag–AV–SF hydrogel developed in this study had good physical properties, overwhelming antibacterial properties, satisfactory biocompatibility and significantly promoting effect on cell proliferation, migration and wound healing. Overall, our results suggest that the Ag–AV–SF hydrogel we developed has great potential for improving the wound healing in clinical treatment.

Fabrication of Turmeric Powder-Loaded Silk Fibroin Film for Wound Healing Application

2020 ◽  
Vol 44 ◽  
pp. 63-72
Author(s):  
Vu Nguyen Doan ◽  
Tung Thanh Truong ◽  
Ha Le Bao Tran
Keyword(s):  
Tensile Strength ◽  
Silk Fibroin ◽  
Wound Dressing ◽  
Chemical Structure ◽  
Research Direction ◽  
Wound Dressings ◽  
Cytotoxicity Test ◽  
Dehydration Rate ◽  
Silk Fibroin Film ◽  
Anti Fungal

Background: Currently silk fibroin is used more and more in the biomedical researches, including a potential research direction in creating wound dressing. Turmeric powder is a natural drug with many properties suitable for treatment of burns such as anti-inflammatory, anti-bacterial, anti-fungal, especially reducing formation of scars. Methods: In this study, sericin is removed from the silk to obtain fibroin fiber. Fibroin fiber and turmeric powder are dissolved by formic acid adding calcium chloride (CaCl2). Created fibroin films (FF) are then evaluated in some characteristics such as surface structure, chemical structure, tensile strength, absorbency, dehydration rate, biodegradation ability, pH determination, preventing bacteria ability and cytotoxicity test. Results: All results indicated that created FF is fulfilled with all the required properties of wound dressings. Conclusions: This study is the first step to creating foundation and orientation for the development of commercial wound dressings.

Silk fibroin-assisted exfoliation and functionalization of transition metal dichalcogenide nanosheets for antibacterial wound dressings

Nanoscale ◽  
2017 ◽  
Vol 9 (44) ◽  
pp. 17193-17198 ◽  
Author(s):  
Xiao-Wei Huang ◽  
Jing-Jing Wei ◽  
Tong Liu ◽  
Xue-Liang Zhang ◽  
Shu-Meng Bai ◽  
...  
Keyword(s):  
Transition Metal ◽  
Aqueous Medium ◽  
Silk Fibroin ◽  
Environmentally Friendly ◽  
Wound Dressings ◽  
Transition Metal Dichalcogenide ◽  
Environmentally Friendly Method

A facile and environmentally friendly method was developed for the efficient exfoliation of TMDs in an aqueous medium by using silk fibroin.

Silver Nanoparticles Incorporated Electrospun Silk Fibers

2007 ◽  
Vol 7 (11) ◽  
pp. 3888-3891 ◽  
Author(s):  
Minsung Kang ◽  
Rira Jung ◽  
Hun-Sik Kim ◽  
Ji Ho Youk ◽  
Hyoung-Joon Jin
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Silver Nanoparticles ◽  
Silk Fibroin ◽  
Wound Dressings ◽  
Silk Fibers ◽  
Transmission Electron ◽  
Electrospun Membranes ◽  
Silk Fibroin Nanofibers ◽  
Fibroin Solution

We present a simple and mass-producible method of incorporating silver nanoparticles on the surface of electrospun silk non-woven membranes for the fabrication of antimicrobial wound dressings. Nanofibrous silk membranes with fiber diameters of 460±40 nm were electrospun from an aqueous Bombyx mori fibroin solution. The electrospun membranes incorporating silver nanoparticles were prepared by dipping the membranes in aqueous silver nitrate (AgNO3) solution (0.5 or 1.0 wt%) followed by photoreduction. Field emission scanning and transmission electron microscopy showed that silver nanoparticles were generated on the electrospun silk fibroin nanofibers as well as inside them. The interaction between the silver nanoparticles and amide groups in the silk fibroin molecules was characterized using X-rayphotoelectron spectroscopy.

scholarly journals Zinc Oxide Nanoparticles Functionalized on Hydrogel Grafted Silk Fibroin Fabrics as Efficient Composite Dressing

Biomolecules ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 710 ◽  
Author(s):  
Sudip Majumder ◽  
Ujjwal Ranjan Dahiya ◽  
Sunny Yadav ◽  
Pratibha Sharma ◽  
Debashree Ghosh ◽  
...  
Keyword(s):  
Zinc Oxide ◽  
Silk Fibroin ◽  
Zinc Oxide Nanoparticles ◽  
Transmission Rate ◽  
Antimicrobial Properties ◽  
Wound Dressings ◽  
Oxide Nanoparticles ◽  
Fibroblast Cells ◽  
L929 Fibroblast

Recent advances in woundcare is targeted towards developing active-dressings, where multiple components are combined to provide a suitable environment for rapid healing. The aim of the present research is to study the preparation of biomimic composite wound dressings by the grafting of hydrogel on silk fibroin fabric. The swelling ability of hydrogel grafted silk fibroin fabric was optimized by changing the initiator concentration. In order to impart antimicrobial properties, these dressing are further coated sono-chemically with zinc oxide nanoparticles. The water vapor transmission rate of the prepared samples was measured. The conformation of silk fibroin proteins after grafting with hydrogel was also confirmed using Fourier Transform Infrared Spectroscopy (FTIR). The morphology of the zinc oxide-coated silk fibroin fabric and hydrogel-coated silk fibroin was studied using Scanning Electron Microscope (SEM). The antimicrobial activity of the zinc oxide-coated samples was studied against E coli. The cytocompatibility of the prepared dressing materials were evaluated using L929 fibroblast cells. MTT assay and phase contrast microscopic studies showed that the adherence, growth, and proliferation of the L929 fibroblast cells that were seeded on zinc oxide nanoparticles on the functionalized hydrogel-coated silk fibroin dressing was significantly higher than that of pure silk fibroin due to the highly porous, bio-mimic structure that allowed ease of passage of nutrients, growth factors, metabolites, and the exchange of gases which is beneficial for successful regeneration of damaged tissues. The expression of TNF-α and IL-2 were not significantly higher than that of control. The proposed composite dressing would be a promising material for wound dressing and regenerative medicine but in order to prove the efficacy of these materials, more in vivo experiments and clinical tests are required to be conducted in future.

Polydopamine modification of silk fibroin membranes significantly promotes their wound healing effect

2019 ◽  
Vol 7 (12) ◽  
pp. 5232-5237 ◽  
Author(s):  
Ying Zhang ◽  
Leihao Lu ◽  
Yuping Chen ◽  
Jie Wang ◽  
Yuyin Chen ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Wound Healing ◽  
Silk Fibroin ◽  
Wound Dressings ◽  
Skin Tissue ◽  
Natural Polymer ◽  
Skin Tissue Engineering ◽  
Healing Effect ◽  
Wound Healing Effect

Natural polymer-based wound dressings have gained great attention in skin tissue engineering.

scholarly journals Fabrication and characterization of an antibacterial chitosan/silk fibroin electrospun nanofiber loaded with a cationic peptide for wound-dressing application

2021 ◽  
Vol 32 (9) ◽  
Author(s):  
Sadjad Khosravimelal ◽  
Milad Chizari ◽  
Behrouz Farhadihosseinabadi ◽  
Mehrdad Moosazadeh Moghaddam ◽  
Mazaher Gholipourmalekabadi
Keyword(s):  
Antibacterial Activity ◽  
Silk Fibroin ◽  
Wound Dressing ◽  
Treatment Strategies ◽  
High Water ◽  
Wound Dressings ◽  
Electrospun Nanofiber ◽  
Cationic Antimicrobial Peptide

AbstractWound infections are still problematic in many cases and demand new alternatives for current treatment strategies. In recent years, biomaterials-based wound dressings have received much attention due to their potentials and many studies have been performed based on them. Accordingly, in this study, we fabricated and optimized an antibacterial chitosan/silk fibroin (CS/SF) electrospun nanofiber bilayer containing different concentrations of a cationic antimicrobial peptide (AMP) for wound dressing applications. The fabricated CS/SF nanofiber was fully characterized and compared to the electrospun silk fibroin and electrospun chitosan alone in vitro. Then, the release rate of different concentrations of peptide (16, 32, and 64 µg/ml) from peptide-loaded CS/SF nanofiber was investigated. Finally, based on cytotoxic activity, the antibacterial activity of scaffolds containing 16 and 32 µg/ml of the peptide was evaluated against standard and multi-drug resistant strains of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa isolated from burn patients. The peptide-loaded CS/SF nanofiber displayed appropriate mechanical properties, high water uptake, suitable biodegradation rate, a controlled release without cytotoxicity on Hu02 human foreskin fibroblast cells at the 16 and 32 µg/ml concentrations of peptide. The optimized CS/SF containing 32 μg/ml peptide showed strong antibacterial activity against all experimental strains from standard to resistance. The results showed that the fabricated antimicrobial nanofiber has the potential to be applied as a wound dressing for infected wound healing, although further studies are needed in vivo.

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