Therapeutic efficacy of an antibiotic-loaded nanosheet in a murine burn-wound infection model

2012 ◽  
Vol 8 (8) ◽  
pp. 2932-2940 ◽  
Author(s):  
Akihiro Saito ◽  
Hiromi Miyazaki ◽  
Toshinori Fujie ◽  
Shinya Ohtsubo ◽  
Manabu Kinoshita ◽  
...  
Keyword(s):  
Wound Infection ◽  
Therapeutic Efficacy ◽  
Infection Model ◽  
Burn Wound

scholarly journals Therapeutic Efficacy of “Nubiotics” against Burn Wound Infection by Pseudomonas aeruginosa

2004 ◽  
Vol 48 (8) ◽  
pp. 2918-2923 ◽  
Author(s):  
Roderic M. K. Dale ◽  
Glen Schnell ◽  
Jonathan P. Wong
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Wound Infection ◽  
Therapeutic Efficacy ◽  
Survival Rates ◽  
Subcutaneous Administration ◽  
Antibacterial Activities ◽  
Infection Model ◽  
Burn Wound

ABSTRACT “Nubiotics” are a novel class of proprietary protonated nucleic acid-based drugs shown to have potent in vitro antibacterial activities against a number of gram-positive and gram-negative bacteria. These nubiotics are evaluated here for their in vivo therapeutic efficacy for the treatment of burn wound infection caused by Pseudomonas aeruginosa. To achieve this, a burn wound infection model was established in mice by using a highly pathogenic burn wound clinical isolate of P. aeruginosa. Lethal doses of the bacteria were determined for two routes of infection (subcutaneous and topical), representing systemic and local forms of infection, respectively. Using this infection model, treatment with nubiotics by various routes of drug administration was evaluated and optimized. A total of 12 nubiotics and their analogues were tested and of these, Nu-2, -3, -4, and -5 were found to be extremely efficacious in the postexposure treatment of burn wound infection (60 to 100% survival rates versus 0% for untreated control [P < 0.05]). These nubiotics were effective when given either systemically by intravenous and/or subcutaneous administration or given locally to the affected site in the skin by topical application. Treatment by these two routes resulted in almost 100% survival rates and complete eradication of the bacteria from infection sites in the livers, spleens, and blood. These nubiotics were found to be as effective as intravenously administered ciprofloxacin, a potent and broad-spectrum fluoroquinolone. These results suggest that nubiotics may be a promising and effective approach for the treatment of burn wound infection caused by P. aeruginosa.

scholarly journals Impact of Frequent Administration of Bacteriophage on Therapeutic Efficacy in an A. baumannii Mouse Wound Infection Model

2020 ◽  
Vol 11 ◽  
Author(s):  
Michael D. Rouse ◽  
Joshua Stanbro ◽  
Jessica A. Roman ◽  
Michelle A. Lipinski ◽  
Anna Jacobs ◽  
...  
Keyword(s):  
Wound Infection ◽  
Therapeutic Efficacy ◽  
Infection Model ◽  
Frequent Administration

INSULIN ATTENUATES THE CYTOKINE RESPONSE IN A BURN WOUND INFECTION MODEL

Shock ◽  
2007 ◽  
Vol 27 (2) ◽  
pp. 205-208 ◽  
Author(s):  
Rene Przkora ◽  
David N. Herndon ◽  
Celeste C. Finnerty ◽  
Marc G. Jeschke
Keyword(s):  
Wound Infection ◽  
Cytokine Response ◽  
Infection Model ◽  
Burn Wound

scholarly journals Antibacterial Efficacy of Silver-Impregnated Polyelectrolyte Multilayers Immobilized on a Biological Dressing in a Murine Wound Infection Model

2012 ◽  
Vol 256 (2) ◽  
pp. 371-377 ◽  
Author(s):  
Kathleen M. Guthrie ◽  
Ankit Agarwal ◽  
Dana S. Tackes ◽  
Kevin W. Johnson ◽  
Nicholas L. Abbott ◽  
...  
Keyword(s):  
Wound Infection ◽  
Polyelectrolyte Multilayers ◽  
Infection Model ◽  
Antibacterial Efficacy ◽  
Biological Dressing

scholarly journals The Microbial Profile and Antibiotic Susceptibility in Burn Wound Infection: A Hospital Based Study

2020 ◽  
pp. 76-86
Author(s):  
P. Denen Akaa ◽  
C. N. Ahachi ◽  
E. Ojo ◽  
G. T. Jombo ◽  
S. Osuji ◽  
...  
Keyword(s):  
Wound Infection ◽  
Antibiotic Susceptibility ◽  
Continuous Process ◽  
Total Body Surface Area ◽  
Antibiotics Resistance ◽  
Burn Wound ◽  
Wound Swab ◽  
Microbial Profile ◽  
Sensitivity Pattern ◽  
Culture And Sensitivity

Aim: To determine spectrum of bacteria infecting burn wound and its antibiotic susceptibility at Benue State University Teaching Hospital (BSUTH). Study Design: A one year prospective study of the microbial profile in burn wound infection at BSUTH, Makurdi. Methodology: All patients admitted from August 2018 to July 2019 with burn wound who had wound swab microscopy culture and sensitivity (MCS) after thorough cleaning of the wound were included in the study. Data collected included the Age, Sex, Occupation, Total Body Surface Area (TBSA) of burn, injuring agent, type of burn, bacteria cultured from the wound, antibiotic susceptibility, number of days of admission and outcome of treatment. Results: Out of the 63 patients admitted, flame was the predominant injuring agent seen in 54 (85.7%) patients. The age group 21 – 30 years was the most affected, 27 constituting 42.9%. Burn wound infection occurred in 25(39.7%) patients from whom 33 bacterial isolates were recovered. Gram negative organisms Pseudomonas species and Proteus species were the commonest bacteria isolated constituting 21.21% each. Other isolates were Staphylococcus aurous 36.36%, Escherichia coli 15.15%, Klebsiella species and Staphylococcus saprophyticus (each 3.03%,). There was multiple bacteria colonization of wounds and antibiotics resistance. There was no gender difference in the burn wound infection, P =0.7819. Conclusion: The sensitivity pattern showed many of the bacteria to be resistant to commonly administered antibiotics but sensitivity patterns got are important for empirical antibiotics prescription when patients come with glaring sepsis and are waiting for wound swab microscopy culture and sensitivity. Studying the microbial profile with antibiotic resistance pattern in burn’s wound infection should be a continuous process in all burn units/departments. This will help to formulate and modify at regular intervals, a hospital/departmental antibiotic policy according to the present microbial pattern in the respective burns unit.    

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