scholarly journals In vitro mineralization of dense collagen substrates: A biomimetic approach toward the development of bone-graft materials

2011 ◽  
Vol 7 (8) ◽  
pp. 3158-3169 ◽  
Author(s):  
Taili T. Thula ◽  
Douglas E. Rodriguez ◽  
Myong Hwa Lee ◽  
Laura Pendi ◽  
Jacob Podschun ◽  
...  
Keyword(s):  
Bone Graft ◽  
In Vitro Mineralization ◽  
Biomimetic Approach

scholarly journals Development and physicochemical characterization of novel porous phosphate glass bone graft substitute and in vitro comparison with xenograft

2021 ◽  
Vol 32 (6) ◽  
Author(s):  
Niketa Chauhan ◽  
Nilay Lakhkar ◽  
Amol Chaudhari
Keyword(s):  
Cell Proliferation ◽  
Physicochemical Properties ◽  
Bone Graft ◽  
Phosphate Glass ◽  
Physicochemical Characterization ◽  
Bone Graft Substitute ◽  
Organic Content ◽  
In Vitro Cytotoxicity ◽  
Porous Phosphate

AbstractThe process of bone regeneration in bone grafting procedures is greatly influenced by the physicochemical properties of the bone graft substitute. In this study, porous phosphate glass (PPG) morsels were developed and their physicochemical properties such as degradation, crystallinity, organic content, surface topography, particle size and porosity were evaluated using various analytical methods. The in vitro cytotoxicity of the PPG morsels was assessed and the interaction of the PPG morsels with Dental Pulp Stem Cells (DPSCs) was studied by measuring cell proliferation and cell penetration depth. The cell-material interactions between PPG morsels and a commercially available xenograft (XG) were compared. The PPG morsels were observed to be amorphous, biocompatible and highly porous (porosity = 58.45%). From in vitro experiments, PPG morsels were observed to be non-cytotoxic and showed better cell proliferation. The internal surface of PPG was easily accessible to the cells compared to XG.

scholarly journals In Vitro Mineralization of Collagen

2021 ◽  
pp. 2004418
Author(s):  
Bernette Maria Oosterlaken ◽  
Maria Paula Vena ◽  
Gijsbertus With
Keyword(s):  
In Vitro Mineralization

In Vitro Mineralization and Cell Adhesion on Surface Modified Poly (2 - hydroxy ethyl methacrylate-co-methyl methacrylate

2006 ◽  
Vol 309-311 ◽  
pp. 493-496 ◽  
Author(s):  
G.S. Sailaja ◽  
T.V. Kumari ◽  
Yoshiyuki Yokogawa ◽  
H.K. Varma
Keyword(s):  
Cell Adhesion ◽  
Calcium Phosphate ◽  
Methyl Methacrylate ◽  
Radical Copolymerization ◽  
Sem Analysis ◽  
Free Radical Copolymerization ◽  
In Vitro Mineralization ◽  
Atr Spectroscopy ◽  
Surface Modified

Poly(2-hydroxyethyl methacrylate-co- methyl methacrylate) HM, was synthesized by free radical copolymerization, cross-linked with ethylene glycol dimethacrylate and phosphorylated. The phosphate coupling was ensured by ATR spectroscopy. The in vitro mineralization ability of the phosphorylated HM (designated as PHM) was investigated by studying the nucleation and growth of calcium phosphate on its surface by immersing in simulated body fluid (SBF) solution. The coating morphology was studied by SEM and the Ca/P ratio of the coating by EDX analysis. The cell adhesion behaviour of PHM was studied by seeding Human osteosarcoma (HOS) cells for one week followed by SEM analysis along with HM as control. It was observed that HOS cells exhibited biomineralization of calcium phosphate on the surface of HM as well as on PHM with a significantly higher amount on the surface of PHM as observed by von kossa staining method. The results show that PHM is capable of in vitro mineralization under simulated physiological condition, promotes cell adhesion by providing an excellent cell friendly surface and it exhibits biomineralization of calcium phosphate in presence of HOS cells.

In Vitro Bio-Mineralization Process

2007 ◽  
Vol 361-363 ◽  
pp. 543-546
Author(s):  
Monica Sandri ◽  
Anna Tampieri ◽  
Luca Bertinetti ◽  
Adele Boskey
Keyword(s):  
Bone Tissue ◽  
Mineralization Process ◽  
Mineral Phase ◽  
Composites Materials ◽  
Physico Chemical ◽  
Biomimetic Approach

The present work describes the development of biomimetic composites materials for bone tissue substitution and repair. At this purpose a biomimetic approach was used and apatitic phases were nucleated on macromolecular matrices like natural collagen, which act as template and induce peculiar physico-chemical features in the mineral phase.

Quantitative Monitoring of In Vitro Mineralization Process using Fluorescent Dyes

2003 ◽  
Vol 240-242 ◽  
pp. 715-718 ◽  
Author(s):  
Motohiro Hirose ◽  
Noriko Kotobuki ◽  
Hiroko Machida ◽  
E. Uchimura ◽  
Hajime Ohgushi
Keyword(s):  
Fluorescent Dyes ◽  
Mineralization Process ◽  
Quantitative Monitoring ◽  
In Vitro Mineralization

In vitro Mineralization by Mesenchymal Stem Cells Cultured on Titanium Scaffolds

2007 ◽  
Vol 141 (5) ◽  
pp. 729-736 ◽  
Author(s):  
Masahiko Maeda ◽  
Motohiro Hirose ◽  
Hajime Ohgushi ◽  
Tadaaki Kirita
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Mineralization ◽  
Cells Cultured

In vitro mineralization of dual grafted polytetrafluoroethylene membranes

2017 ◽  
Vol 12 (2) ◽  
pp. 02C413 ◽  
Author(s):  
Katarzyna Kępa ◽  
David J. T. Hill ◽  
Lisbeth Grøndahl
Keyword(s):  
In Vitro Mineralization
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