In vitro mineralization of dual grafted polytetrafluoroethylene membranes

2017 ◽  
Vol 12 (2) ◽  
pp. 02C413 ◽  
Author(s):  
Katarzyna Kępa ◽  
David J. T. Hill ◽  
Lisbeth Grøndahl
Keyword(s):  
In Vitro Mineralization

scholarly journals In Vitro Mineralization of Collagen

2021 ◽  
pp. 2004418
Author(s):  
Bernette Maria Oosterlaken ◽  
Maria Paula Vena ◽  
Gijsbertus With
Keyword(s):  
In Vitro Mineralization

In Vitro Mineralization and Cell Adhesion on Surface Modified Poly (2 - hydroxy ethyl methacrylate-co-methyl methacrylate

2006 ◽  
Vol 309-311 ◽  
pp. 493-496 ◽  
Author(s):  
G.S. Sailaja ◽  
T.V. Kumari ◽  
Yoshiyuki Yokogawa ◽  
H.K. Varma
Keyword(s):  
Cell Adhesion ◽  
Calcium Phosphate ◽  
Methyl Methacrylate ◽  
Radical Copolymerization ◽  
Sem Analysis ◽  
Free Radical Copolymerization ◽  
In Vitro Mineralization ◽  
Atr Spectroscopy ◽  
Surface Modified

Poly(2-hydroxyethyl methacrylate-co- methyl methacrylate) HM, was synthesized by free radical copolymerization, cross-linked with ethylene glycol dimethacrylate and phosphorylated. The phosphate coupling was ensured by ATR spectroscopy. The in vitro mineralization ability of the phosphorylated HM (designated as PHM) was investigated by studying the nucleation and growth of calcium phosphate on its surface by immersing in simulated body fluid (SBF) solution. The coating morphology was studied by SEM and the Ca/P ratio of the coating by EDX analysis. The cell adhesion behaviour of PHM was studied by seeding Human osteosarcoma (HOS) cells for one week followed by SEM analysis along with HM as control. It was observed that HOS cells exhibited biomineralization of calcium phosphate on the surface of HM as well as on PHM with a significantly higher amount on the surface of PHM as observed by von kossa staining method. The results show that PHM is capable of in vitro mineralization under simulated physiological condition, promotes cell adhesion by providing an excellent cell friendly surface and it exhibits biomineralization of calcium phosphate in presence of HOS cells.

Quantitative Monitoring of In Vitro Mineralization Process using Fluorescent Dyes

2003 ◽  
Vol 240-242 ◽  
pp. 715-718 ◽  
Author(s):  
Motohiro Hirose ◽  
Noriko Kotobuki ◽  
Hiroko Machida ◽  
E. Uchimura ◽  
Hajime Ohgushi
Keyword(s):  
Fluorescent Dyes ◽  
Mineralization Process ◽  
Quantitative Monitoring ◽  
In Vitro Mineralization

In vitro Mineralization by Mesenchymal Stem Cells Cultured on Titanium Scaffolds

2007 ◽  
Vol 141 (5) ◽  
pp. 729-736 ◽  
Author(s):  
Masahiko Maeda ◽  
Motohiro Hirose ◽  
Hajime Ohgushi ◽  
Tadaaki Kirita
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
In Vitro Mineralization ◽  
Cells Cultured

In Vitro Mineralization Study of Chitosan/Carbon Nanotubes Scaffolds: Effect of Mineralization Cycles

2018 ◽  
Vol 378 (1) ◽  
pp. 1600148
Author(s):  
Marilia M. Horn ◽  
Virginia C. Amaro Martins ◽  
Ana M. De Guzzi Plepis
Keyword(s):  
Carbon Nanotubes ◽  
In Vitro Mineralization

Cyclic tensile force stimulates BMP9 synthesis and in vitro mineralization by human periodontal ligament cells

2018 ◽  
Vol 234 (4) ◽  
pp. 4528-4539 ◽  
Author(s):  
Yanee Tantilertanant ◽  
Jitti Niyompanich ◽  
Vincent Everts ◽  
Pitt Supaphol ◽  
Prasit Pavasant ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Tensile Force ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
In Vitro Mineralization

scholarly journals Hedgehog Signaling Inhibition by Smoothened Antagonist BMS-833923 Reduces Osteoblast Differentiation and Ectopic Bone Formation of Human Skeletal (Mesenchymal) Stem Cells

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Nihal AlMuraikhi ◽  
Nuha Almasoud ◽  
Sarah Binhamdan ◽  
Ghaydaa Younis ◽  
Dalia Ali ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bone Formation ◽  
Osteoblast Differentiation ◽  
Global Gene Expression ◽  
Ectopic Bone Formation ◽  
Ectopic Bone ◽  
In Vitro Mineralization ◽  
Global Gene Expression Profiling

Background. Hedgehog (Hh) signaling is essential for osteoblast differentiation of mesenchymal progenitors during endochondral bone formation. However, the critical role of Hh signaling during adult bone remodeling remains to be elucidated. Methods. A Smoothened (SMO) antagonist/Hedgehog inhibitor, BMS-833923, identified during a functional screening of a stem cell signaling small molecule library, was investigated for its effects on the osteoblast differentiation of human skeletal (mesenchymal) stem cells (hMSC). Alkaline phosphatase (ALP) activity and Alizarin red staining were employed as markers for osteoblast differentiation and in vitro mineralization capacity, respectively. Global gene expression profiling was performed using the Agilent® microarray platform. Effects on in vivo ectopic bone formation were assessed by implanting hMSC mixed with hydroxyapatite-tricalcium phosphate granules subcutaneously in 8-week-old female nude mice, and the amount of bone formed was assessed using quantitative histology. Results. BMS-833923, a SMO antagonist/Hedgehog inhibitor, exhibited significant inhibitory effects on osteoblast differentiation of hMSCs reflected by decreased ALP activity, in vitro mineralization, and downregulation of osteoblast-related gene expression. Similarly, we observed decreased in vivo ectopic bone formation. Global gene expression profiling of BMS-833923-treated compared to vehicle-treated control cells, identified 348 upregulated and 540 downregulated genes with significant effects on multiple signaling pathways, including GPCR, endochondral ossification, RANK-RANKL, insulin, TNF alpha, IL6, and inflammatory response. Further bioinformatic analysis employing Ingenuity Pathway Analysis revealed significant enrichment in BMS-833923-treated cells for a number of functional categories and networks involved in connective and skeletal tissue development and disorders, e.g., NFκB and STAT signaling. Conclusions. We identified SMO/Hedgehog antagonist (BMS-833923) as a powerful inhibitor of osteoblastic differentiation of hMSC that may be useful as a therapeutic option for treating conditions associated with high heterotopic bone formation and mineralization.

In vitro mineralization in bovine tooth germ cell cultured with sintered hydroxyapatite

1993 ◽  
Vol 4 (6) ◽  
pp. 569-574 ◽  
Author(s):  
M. Akao ◽  
M. Sakatsume ◽  
H. Aoki ◽  
T. Takagi ◽  
S. Sasaki
Keyword(s):  
Germ Cell ◽  
Tooth Germ ◽  
In Vitro Mineralization
Sign in / Sign up

Export Citation Format

Share Document