Homogeneous biorecognition reaction-induced assembly of DNA nanostructures for ultrasensitive electrochemical detection of kanamycin antibiotic

2021 ◽  
Vol 1154 ◽  
pp. 338317
Author(s):  
Wan Huang ◽  
Yue Zhou ◽  
Danyan Zhan ◽  
Guosong Lai
Keyword(s):  
Electrochemical Detection ◽  
Dna Nanostructures

scholarly journals Immobilization Techniques for Aptamers on Gold Electrodes for the Electrochemical Detection of Proteins: A Review

Biosensors ◽  
2020 ◽  
Vol 10 (5) ◽  
pp. 45
Author(s):  
Franziska V. Oberhaus ◽  
Dieter Frense ◽  
Dieter Beckmann
Keyword(s):  
Electrochemical Detection ◽  
Gold Electrodes ◽  
Dna Nanostructures ◽  
Profound Knowledge ◽  
Low Concentrations ◽  
Reduced Graphene ◽  
Highly Sensitive ◽  
The Subject ◽  
Immobilization Techniques ◽  
Direct Immobilization

The development of reliable biosensing platforms plays a key role in the detection of proteins in clinically and environmentally derived samples for diagnostics, as well as for process monitoring in biotechnological productions. For this purpose, the biosensor has to be stable and reproducible, and highly sensitive to detect potentially extremely low concentrations and prevent the nonspecific binding of interfering compounds. In this review, we present an overview of recently published (2017–2019) immobilization techniques for aptamers on gold electrodes for the electrochemical detection of proteins. These include the direct immobilization of thiolated aptamers and the utilization of short linkers, streptavidin/biotin interaction, as well as DNA nanostructures and reduced graphene oxide as immobilization platforms. Applied strategies for signal amplification and the prevention of biofouling are additionally discussed, as they play a crucial role in the design of biosensors. While a wide variety of amplification strategies are already available, future investigations should aim to establish suitable antifouling strategies that are compatible with electrochemical measurements. The focus of our review lies on the detailed discussion of the underlying principles and the presentation of utilized chemical protocols in order to provide the reader with promising ideas and profound knowledge of the subject, as well as an update on recent discoveries and achievements.

Label-free and dual-amplified electrochemical detection of Hg2+ based on self-assembled DNA nanostructures and target-triggered exonuclease cleavage activity

2016 ◽  
Vol 40 (8) ◽  
pp. 6686-6691 ◽  
Author(s):  
Ting Bao ◽  
Wei Wen ◽  
Lei Shu ◽  
Xiuhua Zhang ◽  
Shengfu Wang
Keyword(s):  
Electrochemical Detection ◽  
Signal Amplification ◽  
Electrochemical Biosensor ◽  
Dna Nanostructures ◽  
Label Free ◽  
Target Recycling ◽  
Cleavage Activity ◽  
Self Assembled ◽  
Exo Iii

An electrochemical biosensor for Hg2+ detection via HCR and Hg2+-triggered Exo III-assisted target recycling for signal amplification.

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