Single identical cell toxicity assay on coordinately ordered patterns

2019 ◽  
Vol 1065 ◽  
pp. 56-63 ◽  
Author(s):  
Qingxuan Li ◽  
Liyuan Ma ◽  
Ming Su
Keyword(s):  
Cell Toxicity ◽  
Toxicity Assay ◽  
Identical Cell

Novel Primary Epithelial Cell Toxicity Assay Using Porcine Corneal Explants

Cornea ◽  
2015 ◽  
Vol 34 (5) ◽  
pp. 567-575 ◽  
Author(s):  
Hiroki Takahashi ◽  
Kazuki Tajima ◽  
Takaaki Hattori ◽  
Naoyuki Yamakawa ◽  
Norihiko Ito ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Toxicity ◽  
Toxicity Assay ◽  
Primary Epithelial Cell

scholarly journals Inhibition of the filamentation of Candida albicans by Borojoa patinoi silver nanoparticles

2021 ◽  
Vol 3 (2) ◽  
Author(s):  
Marcela Gómez-Garzón ◽  
Luz D. Gutiérrez-Castañeda ◽  
Camilo Gil ◽  
Carlos H. Escobar ◽  
Ana P. Rozo ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Candida Albicans ◽  
Green Synthesis ◽  
Cell Line ◽  
Opportunistic Pathogen ◽  
Inhibitory Effect ◽  
Cell Toxicity ◽  
Toxicity Assay ◽  
Filamentous Form ◽  
Shape And Size

AbstractCandida albicans is fungus capable of changing from yeast to filamentous form when it’s transformed from a normal commensal to an opportunistic pathogen. The development of alternatives that interfere with this transition could be an effective way to reduce candidiasis. In this regard, evaluate the inhibitory effect of two Borojoa patinoi silver nanoparticles (AgNPs) produced by green synthesis at 5 °C and 25 °C on the process of filamentation of Candida albicans. The percentage of inhibition of filamentous forms of C. albicans ATCC10231 and C. albicans SC5314 with AgNPs was determined. Results showed that temperature of synthesis affected both the shape and size of silver nanoparticles synthesized using Borojoa patinoi extracts. The inhibition percentage of filamentous forms of Candida albicans ATCC10231 when treated with silver nanoparticles synthesized at 5 °C was 85.9% and at 25 °C it was 40%. C. albicans SC5314 when treated with AgNP synthesized at 5 °C was 97.2% and at 25 °C it was 64%. Cell toxicity assay showed that at 100ng/ml, AgNPs synthesized at 25 °C were safe in MES-OV CRL-3272 cell line. Our results showed that the silver nanoparticles obtained from Borojoa patinoi are inhibitors of the filamentous process of C. albicans.

scholarly journals Development of a highly sensitive in vitro endothelial cell toxicity assay for evaluating ricin toxin A chain-based vaccines or therapeutics

Toxicon ◽  
2019 ◽  
Vol 167 ◽  
pp. 152-161 ◽  
Author(s):  
Nicholas J. Machesky ◽  
Janice M. Rusnak ◽  
Evan H. Moore ◽  
Christopher B. Dorsey ◽  
Lucy A. Ward
Keyword(s):  
Endothelial Cell ◽  
Cell Toxicity ◽  
Toxin A ◽  
Toxicity Assay ◽  
Ricin Toxin ◽  
Highly Sensitive ◽  
A Chain

Reproducibility and Robustness of a Real-Time Microfluidic Cell Toxicity Assay

2011 ◽  
Vol 83 (10) ◽  
pp. 3890-3896 ◽  
Author(s):  
Gregory A. Cooksey ◽  
John T. Elliott ◽  
Anne L. Plant
Keyword(s):  
Real Time ◽  
Cell Toxicity ◽  
Toxicity Assay

Two- or three-way observations of the identical cell elements within the same sections by LM, TEM and/or SEM

1978 ◽  
Vol 36 (2) ◽  
pp. 82-83 ◽  
Author(s):  
Nakazo Watari ◽  
Yasuaki Hotta ◽  
Yoshio Mabuchi
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Fine Structure ◽  
Light Microscopy ◽  
Thin Sections ◽  
Transmission Electron ◽  
Identical Cell ◽  
Electron Microscopes ◽  
Scanning Electron

It is very useful if we can observe the identical cell elements within the same sections by light microscopy (LM), transmission electron microscopy (TEM) and/or scanning electron microscopy (SEM) sequentially, because, the cell fine structure can not be indicated by LM, while the color is; on the other hand, the cell fine structure can be very easily observed by EM, although its color properties may not. However, there is one problem in that LM requires thick sections of over 1 μm, while EM needs very thin sections of under 100 nm. Recently, we have developed a new method to observe the same cell elements within the same plastic sections using both light and transmission (conventional or high-voltage) electron microscopes.In this paper, we have developed two new observation methods for the identical cell elements within the same sections, both plastic-embedded and paraffin-embedded, using light microscopy, transmission electron microscopy and/or scanning electron microscopy (Fig. 1).

Design of Dermaseptin S3 Analogues having Bacterial Cell Selectivity without Mammalian Cell Toxicity

2001 ◽  
Vol 8 (4) ◽  
pp. 281-288 ◽  
Author(s):  
Song Shin ◽  
Sung Yang ◽  
Soo Eom ◽  
Woo Song ◽  
Yangmee Kim ◽  
...  
Keyword(s):  
Mammalian Cell ◽  
Bacterial Cell ◽  
Cell Toxicity ◽  
Cell Selectivity ◽  
Mammalian Cell Toxicity

DNA Binding Mode of Transition Metal Complexes, A Relationship to Tumor Cell Toxicity

2014 ◽  
Vol 21 (26) ◽  
pp. 3081-3094 ◽  
Author(s):  
M. Ashfaq ◽  
T. Najam ◽  
S.S.A. Shah ◽  
M.M. Ahmad ◽  
S. Shaheen ◽  
...  
Keyword(s):  
Dna Binding ◽  
Transition Metal ◽  
Tumor Cell ◽  
Metal Complexes ◽  
Transition Metal Complexes ◽  
Binding Mode ◽  
Cell Toxicity

Targeted Nanostructured Lipid Carriers for Delivery of Paclitaxel to Cancer Cells: Preparation, Characterization, and Cell Toxicity

2017 ◽  
Vol 14 (8) ◽  
Author(s):  
Mahboubeh Rezazadeh ◽  
Jaber Emami ◽  
Farshid Hassanzadeh ◽  
Hojjat Sadeghi ◽  
Mahboubeh Rostami ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Nanostructured Lipid Carriers ◽  
Cell Toxicity

scholarly journals The synergistic interference effect of silica nanoparticles concentration and the wavelength of ELISA on the colorimetric assay of cell toxicity

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fariba Abbasi ◽  
Hassan Hashemi ◽  
Mohammad Reza Samaei ◽  
Amir SavarDashtaki ◽  
Abooalfazl Azhdarpoor ◽  
...  
Keyword(s):  
Mtt Assay ◽  
Interference Effect ◽  
Polynomial Regression ◽  
Colorimetric Assay ◽  
Synergistic Effects ◽  
Cell Toxicity ◽  
Diphenyltetrazolium Bromide ◽  
Nanoparticles Concentration ◽  
Artificial Neural Network Ann ◽  
First Time

AbstractThe 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay is the most common method for the determination of cell toxicity, but some factors limit the sensitivity of this method, such as pH. Less attention had been paid to the interference effect of optical and plasmonic properties of SiO2 nanoparticles (NPs) in the wavelength range assigned to MTT. This study investigated the synergistic interference effect of SiO2 NPs and wavelength on MTT assay for the first time. The examined variables included the type of SiO2 NPs concentrations (1, 10, and 100 mM) and different wavelengths (470, 490, 520, and 570 nm). The results showed that optical density (OD) increased (p < 0.05) when wavelength and the concentration of crystalline SiO2 NPs increased. So, the maximum OD at 10 and 100 mM were attributed to crystalline SiO2 NPs (p < 0.05) due to the functional group, whereas it was related to amorphous at 1 mM (p > 0.05). According to polynomial regression modeling (PRM), the maximum interference effect was predicted at crystalline SiO2 NPs and wavelength > 550 nm. Besides, the synergistic effects of SiO2 NPs, wavelength, and concentration of NPs had been a good fitting with first-order PRM. Thus, the concentration of SiO2 NPs had a confounder factor in colorimetric for MTT assay. The best artificial neural network (ANN) structure was related to the 3:7:1 network (Rall = 0.936, MSE = 0.0006, MAPE = 0.063). The correlation between the actual and predicted data was 0.88. As SiO2 NPs presence is an interfering factor in MTT assay concerning wavelength, it is suggested wavelength use with minimum confounding effect for MTT assay.

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