Time-resolved chemiluminescence: a novel tool for improved emission sequence in stopped-flow analysis

2004 ◽  
Vol 517 (1-2) ◽  
pp. 111-117 ◽  
Author(s):  
José A Murillo Pulgarı́n ◽  
Luisa F Garcı́a Bermejo ◽  
José A Rubio Aranda
Keyword(s):  
Flow Analysis ◽  
Stopped Flow ◽  
Time Resolved

scholarly journals Time-resolved detection of SDS-induced conformational changes in α-synuclein by a micro-stopped-flow system

RSC Advances ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 1086-1097
Author(s):  
Shunki Takaramoto ◽  
Yusuke Nakasone ◽  
Kei Sadakane ◽  
Shinsaku Maruta ◽  
Masahide Terazima
Keyword(s):  
Conformational Changes ◽  
Flow System ◽  
Stopped Flow ◽  
Time Resolved ◽  
Sds Micelles ◽  
Conformation Changes ◽  
System P

Dynamics of conformation changes of α-synuclein induced by the presence of SDS micelles are revealed using time-resolved diffusion, CD, and FRET measurements combined with a micro-stopped flow system.

scholarly journals Stopped-flow microscopy for time resolved shape change observation of floating erythrocyte

1996 ◽  
Vol 36 (6) ◽  
pp. 293-296
Author(s):  
Takayuki SANO ◽  
Kenji SATO ◽  
Katsumasa INOUE
Keyword(s):  
Shape Change ◽  
Stopped Flow ◽  
Time Resolved

Time-Resolved Binding of Carbon Monoxide to Nitrogenase Monitored by Stopped-Flow Infrared Spectroscopy

1997 ◽  
Vol 119 (27) ◽  
pp. 6450-6451 ◽  
Author(s):  
Simon J. George ◽  
Gillian A. Ashby ◽  
Christopher W. Wharton ◽  
Roger N. F. Thorneley
Keyword(s):  
Carbon Monoxide ◽  
Infrared Spectroscopy ◽  
Stopped Flow ◽  
Time Resolved

Stopped-flow analysis on the mechanism of perylene nanoparticle formation by the reprecipitation method

2009 ◽  
Vol 311 (3) ◽  
pp. 553-555 ◽  
Author(s):  
Junichi Mori ◽  
Yousuke Miyashita ◽  
Daniel Oliveira ◽  
Hitoshi Kasai ◽  
Hidetoshi Oikawa ◽  
...  
Keyword(s):  
Flow Analysis ◽  
Stopped Flow ◽  
Nanoparticle Formation ◽  
Reprecipitation Method

A Simulation of the Unsteady Interaction of a Centrifugal Impeller With its Vaned Diffuser: Flows Analysis

1994 ◽  
Author(s):  
W. N. Dawes
Keyword(s):  
Flow Analysis ◽  
Three Dimensional ◽  
Navier Stokes ◽  
Centrifugal Impeller ◽  
Vaned Diffuser ◽  
Time Resolved ◽  
High Loss ◽  
Swirl Angle ◽  
Unsteady Interaction ◽  
Unsteady Effects

The aim of this paper is to help advance our understanding of the complex, three-dimensional, unsteady flow associated with the interaction of a splattered centrifugal impeller and its vaned diffuser. A time-resolved simulation is presented of the Krain stage performed using a time-accurate, 3D, unstructured mesh, solution-adaptive Navier-Stokes solver. The predicted flowfield, compared with experiment where available, displays a complex, unsteady interaction especially in the neighbourhood of the diffuser entry zone which experiences large periodic flow unsteadiness. Downstream of the throat, although the magnitude of this unsteadiness diminishes rapidly, the flow has a highly distorted three-dimensional character. The loss levels in the diffuser are then investigated to try and determine how time-mean loss levels compare with the levels expected from “equivalent” steady flow analysis performed by using the circumferentially averaged exit flow from the impeller as inlet to the diffuser. It is concluded that little loss could be attributed directly to unsteady effects but rather that the principle cause of the rather high loss levels observed in the diffuser is the strong spanwise distortion in swirl angle at inlet which initiates a strong hub/comer stall.

Human RhAG ammonia channel is impaired by the Phe65Ser mutation in overhydrated stomatocytic red cells

2012 ◽  
Vol 302 (2) ◽  
pp. C419-C428 ◽  
Author(s):  
Sandrine Genetet ◽  
Pierre Ripoche ◽  
Julien Picot ◽  
Sylvain Bigot ◽  
Jean Delaunay ◽  
...  
Keyword(s):  
Intracellular Ph ◽  
Hemolytic Anemia ◽  
Rate Constant ◽  
Rate Constants ◽  
Flow Analysis ◽  
Red Cells ◽  
Stopped Flow ◽  
Loss Of Function ◽  
Dramatic Decrease ◽  
Time Courses

In red cells, Rh-associated glycoprotein (RhAG) acts as an ammonia channel, as demonstrated by stopped-flow analysis of ghost intracellular pH (pHi) changes. Recently, overhydrated hereditary stomatocytosis (OHSt), a rare dominantly inherited hemolytic anemia, was found to be associated with a mutation (Phe65Ser or Ile61Arg) in RHAG. Ghosts from the erythrocytes of four of the OHSt patients with a Phe65Ser mutation were resealed with a pH-sensitive probe and submitted to ammonium gradients. Alkalinization rate constants, reflecting NH3transport through the channel and NH3diffusion unmediated by RhAG, were deduced from time courses of fluorescence changes. After subtraction of the constant value found for Rhnulllacking RhAG, we observed that alkalinization rate constant values decreased ∼50% in OHSt compared with those of controls. Similar RhAG expression levels were found in control and OHSt. Since half of the expressed RhAG in OHSt most probably corresponds to the mutated form of RhAG, as expected from the OHSt heterozygous status, this dramatic decrease can be therefore related to the loss of function of the Phe65Ser-mutated RhAG monomer.

Stopped-Flow Analysis on Anion Binding to Blue-Form Halorhodopsin fromNatronobacterium pharaonis:  Comparison with the Anion-Uptake Process during the Photocycle†

Biochemistry ◽  
2002 ◽  
Vol 41 (7) ◽  
pp. 2452-2458 ◽  
Author(s):  
Maki Sato ◽  
Tatsuaki Kanamori ◽  
Naoki Kamo ◽  
Makoto Demura ◽  
Katsutoshi Nitta
Keyword(s):  
Flow Analysis ◽  
Anion Binding ◽  
Stopped Flow ◽  
Uptake Process ◽  
Anion Uptake
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