scholarly journals Stopped-flow microscopy for time resolved shape change observation of floating erythrocyte

1996 ◽  
Vol 36 (6) ◽  
pp. 293-296
Author(s):  
Takayuki SANO ◽  
Kenji SATO ◽  
Katsumasa INOUE
Keyword(s):  
Shape Change ◽  
Stopped Flow ◽  
Time Resolved

scholarly journals Time-resolved detection of SDS-induced conformational changes in α-synuclein by a micro-stopped-flow system

RSC Advances ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 1086-1097
Author(s):  
Shunki Takaramoto ◽  
Yusuke Nakasone ◽  
Kei Sadakane ◽  
Shinsaku Maruta ◽  
Masahide Terazima
Keyword(s):  
Conformational Changes ◽  
Flow System ◽  
Stopped Flow ◽  
Time Resolved ◽  
Sds Micelles ◽  
Conformation Changes ◽  
System P

Dynamics of conformation changes of α-synuclein induced by the presence of SDS micelles are revealed using time-resolved diffusion, CD, and FRET measurements combined with a micro-stopped flow system.

Time-Resolved Binding of Carbon Monoxide to Nitrogenase Monitored by Stopped-Flow Infrared Spectroscopy

1997 ◽  
Vol 119 (27) ◽  
pp. 6450-6451 ◽  
Author(s):  
Simon J. George ◽  
Gillian A. Ashby ◽  
Christopher W. Wharton ◽  
Roger N. F. Thorneley
Keyword(s):  
Carbon Monoxide ◽  
Infrared Spectroscopy ◽  
Stopped Flow ◽  
Time Resolved

Salt-induced cluster formation of gold nanoparticles followed by stopped-flow SAXS, DLS and extinction spectroscopy

2017 ◽  
Vol 19 (25) ◽  
pp. 16348-16357 ◽  
Author(s):  
Nico Carl ◽  
Sylvain Prévost ◽  
Joseph P. S. Fitzgerald ◽  
Matthias Karg
Keyword(s):  
Gold Nanoparticles ◽  
Cluster Formation ◽  
Stopped Flow ◽  
Time Resolved

The salt-induced cluster formation of negatively charged gold nanoparticles was studied by time-resolved DLS, stopped-flow SAXS and in-situ extinction spectroscopy.

scholarly journals Time-resolved universal temperature measurements using NaYF4:Er3+,Yb3+ upconverting nanoparticles in an electrospray jet

2018 ◽  
Vol 9 ◽  
pp. 2916-2924 ◽  
Author(s):  
Kristina Shrestha ◽  
Arwa A Alaulamie ◽  
Ali Rafiei Miandashti ◽  
Hugh H Richardson
Keyword(s):  
Temperature Measurement ◽  
Environmental Changes ◽  
Shape Change ◽  
Fluid Velocity ◽  
Taylor Cone ◽  
Temperature Measurements ◽  
Acquisition Time ◽  
Peak Shape ◽  
Time Resolved ◽  
Universal Calibration

Hexagonal upconverting nanoparticles (UCNPs) of NaYF4:Er3+,Yb3+ (ca. 300 nm) have been widely used to measure the temperature at the nanoscale using luminescence ratio thermometry. However, several factors limit their applications. For example, changes in the peak shape, mainly is the S-band emission, hinders their ability to be used as a universal temperature sensor. Herein, we introduce a universal calibration protocol for NaYF4:Er3+,Yb3+ upconverting nanoparticles that is robust to environmental changes and gives a precise temperature measurement. We used this new procedure to calculate the temperature profile inside a Taylor cone generated with an electrospray jet. Inside the Taylor cone the fluid velocity increases toward the tip of the cone. A constant acquisition length leads to a decrease in excitation and acquisition time. This decrease in excitation time causes a peak shape change that corrupts the temperature measurement if the entire peak shape is integrated in the calibration. Our universal calibration circumvents this problem and can be used for time-resolved applications. The temperature at the end of the Taylor cone increases due to the creation of a whispering gallery mode cavity with 980 nm excitation. We use time-resolved energy balance equations to support our optical temperature measurements inside the Taylor cone. We believe that the findings of this paper provide a foundation for time-resolved temperature measurements using NaYF4:Er3+,Yb3+ upconverting nanoparticles and can be used to understand temperature-dependent reactions such as protein unfolding inside microjet/microdroplets and microfluidic systems.

scholarly journals Structural Kinetics of MsbA Investigated by Stopped-Flow Time-Resolved Small-Angle X-Ray Scattering

Structure ◽  
2020 ◽  
Vol 28 (3) ◽  
pp. 348-354.e3 ◽  
Author(s):  
Inokentijs Josts ◽  
Yunyun Gao ◽  
Diana C.F. Monteiro ◽  
Stephan Niebling ◽  
Julius Nitsche ◽  
...  
Keyword(s):  
Small Angle ◽  
Flow Time ◽  
Stopped Flow ◽  
Time Resolved ◽  
X Ray ◽  
X Ray Scattering ◽  
Kinetics Of ◽  
Ray Scattering
Sign in / Sign up

Export Citation Format

Share Document