scholarly journals Design and structural characterisation of monomeric water-soluble α-helix and β-hairpin peptides: State-of-the-art

2019 ◽  
Vol 661 ◽  
pp. 149-167 ◽  
Author(s):  
Paula Morales ◽  
M. Angeles Jiménez
Keyword(s):  
State Of The Art ◽  
Water Soluble ◽  
Structural Characterisation ◽  
Hairpin Peptides ◽  
Α Helix

Expedient route to functionalized and water soluble 5-6-5 imidazole-phenyl-thiazole based α-helix mimetics

Tetrahedron ◽  
2013 ◽  
Vol 69 (5) ◽  
pp. 1663-1668 ◽  
Author(s):  
Christopher G. Cummings ◽  
Andrew D. Hamilton
Keyword(s):  
Water Soluble ◽  
Helix Mimetics ◽  
Α Helix

EDC-Crosslinked Electrospun Silk-Fibroin Fiber Mats

2011 ◽  
Vol 175-176 ◽  
pp. 170-175 ◽  
Author(s):  
Rong Liu ◽  
Feng Zhang ◽  
Bao Qi Zuo ◽  
Huan Xiang Zhang
Keyword(s):  
Silk Fibroin ◽  
Rupture Strength ◽  
Mechanical Test ◽  
Crosslinking Agent ◽  
Water Soluble ◽  
Random Coil ◽  
Crosslinking Reaction ◽  
Elongation Ratio ◽  
Silk Fibroin Fiber ◽  
Α Helix

Electrospun Silk-Fibroin (SF) mats were fabricated by electrospinning with regenerated Bombyx mori silk-fibroin/formic acid solutions. After spinning, the water soluble and mechanical properties of pure fibroin nanofibers were poor. So electrospun SF mats were crosslinked with N-(3-dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC), a low cell cytotoxicity crosslinking agent, and N-hydroxysuccinimide (NHS), which can increase the reaction rate. The scanning electron microscope images indicated that the diameter of fibers increased with crosslinking reaction. When EDC/NHS reached to 7.5wt.%, the diameter of fibers achieved the maximum. The mechanical test showed that tensile strength enhanced after crosslinking with EDC/ NHS. While EDC/NHS reached to 7.5wt %, the rupture strength reached to (38.31±5.30) Mpa, and the breaking elongation ratio reached to (182.00±31.27) %. FTIR results showed the the proportion of β-sheet increased while random coil and α-helix decreased after treatment.

scholarly journals Constructing synthetic-protein assemblies from de novo designed 310 helices

2021 ◽  
Author(s):  
Prasun Kumar ◽  
Neil G. Paterson ◽  
Jonathan Clayden ◽  
Derek N. Woolfson
Keyword(s):  
Rational Design ◽  
De Novo ◽  
Protein Structures ◽  
Water Soluble ◽  
Design Solution ◽  
Protein Assemblies ◽  
Synthetic Protein ◽  
Hydrophobic Cores ◽  
Α Helix ◽  
Helical Folding

Compared with the iconic α helix, 310 helices occur much less frequently in protein structures. The different 310-helical parameters lead to energetically less favourable internal energies, and a reduced tendency to pack into defined higher-order structures. Consequently, in natural proteins, 310 helices rarely extend past 6 residues, and do not form regular supersecondary, tertiary, or quaternary interactions. Here, we show that despite their absence in nature, synthetic protein-like assemblies can be built from 310 helices. We report the rational design, solution-phase characterisation, and an X-ray crystal structure for water-soluble bundles of 310 helices with consolidated hydrophobic cores. The design uses 6-residue repeats informed by analysing natural 310 helices, and incorporates aminoisobutyric acid residues. Design iterations reveal a tipping point between α-helical and 310-helical folding, and identify features required for stabilising assemblies in this unexplored region of protein-structure space.

scholarly journals Anthocyanins: A Comprehensive Review of Their Chemical Properties and Health Effects on Cardiovascular and Neurodegenerative Diseases

Molecules ◽  
2020 ◽  
Vol 25 (17) ◽  
pp. 3809 ◽  
Author(s):  
Roberto Mattioli ◽  
Antonio Francioso ◽  
Luciana Mosca ◽  
Paula Silva
Keyword(s):  
Neurodegenerative Diseases ◽  
Nutritional Value ◽  
Fruits And Vegetables ◽  
State Of The Art ◽  
Chemical Properties ◽  
Water Soluble ◽  
Phase Ii Metabolism ◽  
Comprehensive Review ◽  
Natural Colorants ◽  
Health Promoting

Anthocyanins are a class of water-soluble flavonoids widely present in fruits and vegetables. Dietary sources of anthocyanins include red and purple berries, grapes, apples, plums, cabbage, or foods containing high levels of natural colorants. Cyanidin, delphinidin, malvidin, peonidin, petunidin, and pelargonidin are the six common anthocyanidins. Following consumption, anthocyanin, absorption occurs along the gastrointestinal tract, the distal lower bowel being the place where most of the absorption and metabolism occurs. In the intestine, anthocyanins first undergo extensive microbial catabolism followed by absorption and human phase II metabolism. This produces hybrid microbial–human metabolites which are absorbed and subsequently increase the bioavailability of anthocyanins. Health benefits of anthocyanins have been widely described, especially in the prevention of diseases associated with oxidative stress, such as cardiovascular and neurodegenerative diseases. Furthermore, recent evidence suggests that health-promoting effects attributed to anthocyanins may also be related to modulation of gut microbiota. In this paper we attempt to provide a comprehensive view of the state-of-the-art literature on anthocyanins, summarizing recent findings on their chemistry, biosynthesis, nutritional value and on their effects on human health.

scholarly journals Structure Water-Solubility Relationship in α-Helix-Rich Films Cast from Aqueous and 1,1,1,3,3,3-Hexafluoro-2-Propanol Solutions of S. c. ricini Silk Fibroin

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3945
Author(s):  
Moseti ◽  
Yoshioka ◽  
Kameda ◽  
Nakazawa
Keyword(s):  
Silk Fibroin ◽  
Water Solubility ◽  
Silk Gland ◽  
Post Treatment ◽  
Water Soluble ◽  
Cast Film ◽  
Silk Glands ◽  
Α Helix ◽  
Β Sheet ◽  
Structure Water

Silk fibroin (SF) produced by the domesticated wild silkworm, Samia cynthia ricini (S. c. ricini) is attracting increasing interest owing to its unique mechanical properties, biocompatibility, and abundance in nature. However, its utilization is limited, largely due to lack of appropriate processing strategies. Various strategies have been assessed to regenerate cocoon SF, as well as the use of aqueous liquid fibroin (LFaq) prepared by dissolution of silk dope obtained from the silk glands of mature silkworm larvae in water. However, films cast from these fibroin solutions in water or organic solvents are often water-soluble and require post-treatment to render them water-stable. Here, we present a strategy for fabrication of water-stable films from S. c. ricini silk gland fibroin (SGF) without post-treatment. Aqueous ethanol induced gelation of fibroin in the posterior silk glands (PSG), enabling its separation from the rest of the silk gland. When dissolved in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP), the SGF-gel gave a solution from which a transparent, flexible, and water-insoluble film (SGFHFIP) was cast. Detailed structural characterization of the SGFHFIP as-cast film was carried out and compared to a conventional, water-soluble film cast from LFaq. FTIR and 13C solid-state NMR analyses revealed both cast films to be α-helix-rich. However, gelation of SGF induced by the 40%-EtOH-treatment resulted in an imperfect β-sheet structure. As a result, the SGF-gel was soluble in HFIP, but some β-sheet structural memory remains, and the SGFHFIP as-cast film obtained has some β-sheet content which renders it water-resistant. These results reveal a structure water-solubility relationship in S. c. ricini SF films that may offer useful insights towards tunable fabrication of novel biomaterials. A plausible model of the mechanism that leads to the difference in water resistance of the two kinds of α-helix-rich films is proposed.

Synthesis, structural characterisation, and cytotoxicity studies of Bi, W, and Mo containing homo- and hetero-bimetallic polyoxometalates

2019 ◽  
Vol 48 (42) ◽  
pp. 15962-15969 ◽  
Author(s):  
Dimuthu C. Senevirathna ◽  
Melissa V. Werrett ◽  
Manja Kubeil ◽  
Holger Stephan ◽  
Philip C. Andrews
Keyword(s):  
Water Soluble ◽  
Embryonic Cells ◽  
Human Carcinoma ◽  
Structural Characterisation ◽  
Cytotoxicity Studies ◽  
Low Toxicity

High nuclearity, water soluble polyoxometalates composed of high Z number metals W, Mo and Bi demonstrate low toxicity to human carcinoma and healthy embryonic cells and their potential as radiosensitisers.

Conformational Exchange on the Microsecond Time Scale in α-Helix and β-Hairpin Peptides Measured by13C NMR Transverse Relaxation†

Biochemistry ◽  
2001 ◽  
Vol 40 (9) ◽  
pp. 2844-2853 ◽  
Author(s):  
Irina Nesmelova ◽  
Alexei Krushelnitsky ◽  
Djaudat Idiyatullin ◽  
Francesco Blanco ◽  
Marina Ramirez-Alvarado ◽  
...  
Keyword(s):  
Time Scale ◽  
Conformational Exchange ◽  
Transverse Relaxation ◽  
Hairpin Peptides ◽  
Α Helix

Alpha and beta conformations of water soluble sequential iso polypeptides

1988 ◽  
Vol 53 (11) ◽  
pp. 2825-2832 ◽  
Author(s):  
Bernard Barbier ◽  
Margarita Perello ◽  
André Brack
Keyword(s):  
Circular Dichroism ◽  
Water Soluble ◽  
Helical Conformation ◽  
Molecular Weights ◽  
Helix Formation ◽  
Α Helix ◽  
Circular Dichroïsm ◽  
Β Sheet ◽  
Protected Peptides

Alternating poly(Leu-Lys) and its isopolypeptide poly(Leu-Lys-Lys-Leu) were synthesized via polycondensation of p-nitrophenyl esters of the corresponding protected peptides. Addition of one equivalent of 1-hydroxybenzotriazole and varying amounts of a tertiary base allowed to control the molecular weights of the samples. The conformation of the water soluble polypeptides was investigated by circular dichroism. Poly(Leu-Lys) adopts a β-sheet conformation in the presence of salt while poly(Leu-Lys-Lys-Leu) adopts an α-helical conformation. For polypeptides based on a 1 : 1 composition of hydrophobic (A) and hydrophilic (B) residues, the shortest repeat for the formation of a β-sheet is -AB- whereas -AABB- represents the shortest repeat for an α-helix formation.

Structural characterisation of a water-soluble polysaccharide from tissue-cultured Dendrobium huoshanense C.Z. Tang et S.J. Cheng

2017 ◽  
Vol 32 (3) ◽  
pp. 252-260 ◽  
Author(s):  
Hua-Yang Si ◽  
Nai-Fu Chen ◽  
Nai-Dong Chen ◽  
Cheng Huang ◽  
Jun Li ◽  
...  
Keyword(s):  
Water Soluble ◽  
Structural Characterisation ◽  
Soluble Polysaccharide ◽  
Dendrobium Huoshanense
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