Molecular and functional characterization of choline transporter in human colon carcinoma HT-29 cells

2009 ◽  
Vol 483 (1) ◽  
pp. 90-98 ◽  
Author(s):  
Hironobu Kouji ◽  
Masato Inazu ◽  
Tomoko Yamada ◽  
Hirohisa Tajima ◽  
Tatsuya Aoki ◽  
...  
1990 ◽  
Vol 10 (3) ◽  
pp. 293-299 ◽  
Author(s):  
Ewa Rydell ◽  
Karl-Eric Magnusson ◽  
Anita Sjö ◽  
Krister Axelsson

Protein kinase C (PK-C) and casein kinase II (CK-II) activities were studied in two human colon carcinoma cell lines (HT-29 and CaCO-2) undergoing differentiation in vitro resulting, in small-intestine-like cells. CaCo-2 cells, when grown under standard conditions, appear to undergo spontaneous differentiation. In these cells PK-C and CK-II activities were determined on day 5, 10 and 15. No significant differences in activities were seen either in PK-C or CK-II activity. HT-29 cells, when grown in glucose-free medium can be stimulated to undergo differentiation which is completed within 20 days. PK-C and CK-II activities were determined after 5, 10, 15, 20 and 25 days, respectively. PK-C activity rose from 7.9±3.5 pmole32P/mg protein/min at day 5 to 37.5±14.8 pmole32P/mg protein/min at day 20. After 25 days the activity was reduced to 20.0±7.8 pmole32P/mg protein/min. CK-II activity did not change significantly during day 5 to 20, but on day 25 there was a significant decrease in CK-II activity from 94.9±6.4 pmole32P/mg protein/min (day 20) to 62.6±3.9 pmole32P/mg protein/min (day 25) p=0.003. The results in this study indicate a role for PK-C and CK-II in cell growth and differentiation.


1983 ◽  
Vol 47 (3) ◽  
pp. 373-381 ◽  
Author(s):  
M G Brattain ◽  
M E Marks ◽  
J McCombs ◽  
W Finely ◽  
D E Brattain

Life Sciences ◽  
2000 ◽  
Vol 68 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Kamran Gharehbaghi ◽  
Thomas Szekeres ◽  
Joel A. Yalowitz ◽  
Monika Fritzer-Szekeres ◽  
Yves G. Pommier ◽  
...  

1990 ◽  
Vol 40 (8) ◽  
pp. 1779-1783 ◽  
Author(s):  
Matsumoto Masahiko ◽  
John G. Fox ◽  
Wang Pou-Hsiung ◽  
Phanesh B. Koneru ◽  
Eric J. Lien ◽  
...  

2017 ◽  
Vol 141 (5) ◽  
pp. 3492-3492
Author(s):  
Amy Longstreth ◽  
Judene Thomas ◽  
Yaa Kwakwa ◽  
Janae Davis ◽  
Maria-Teresa Herd

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